Home Primary Antibodies Myc tag Antibody [A14H15]

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Myc tag Antibody [A14H15]

Catalog No.: F4919

    Application: Reactivity:

    Usage Information

    Dilution
    1:500 - 1:1000
    1:400
    1:2000
    Application
    WB, IP. IHC, IF, FCM
    Reactivity
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    48 kDa,49 kDa 48 kDa, 13 kDa, 45 kDa, 57 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    Myc tag Antibody [A14H15] detects recombinant proteins containing the Myc epitope tag.
    Clone
    A14H15
    Synonym(s)
    BHLHE39; MYC; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; Transcription factor p64; bHLHe39
    Background
    The Myc tag is a widely used small epitope tag consisting of an 11-amino-acid peptide sequence (MEQKLISEEDL) derived from the c-Myc protein, belonging to the family of epitope tags used for labeling and detecting recombinant proteins across various expression systems, including bacteria, yeast, insect, and mammalian cells. The Myc tag is compact and hydrophilic, which minimizes interference with the native structure and function of the fused protein. It is genetically fused to target proteins via recombinant DNA technology, allowing easy detection with highly specific monoclonal antibodies. It enables diverse applications such as immunoblotting (Western blotting) to detect protein expression and size, immunoprecipitation for isolating protein complexes, immunostaining for visualizing localization in cells, and flow cytometry for quantification. Its small size (<2 kDa) ensures minimal perturbation of the target protein. The Myc tag can also be combined with other tags, enhancing purification and interaction studies, thus simplifying protein characterization and accelerating research in cellular and molecular biology.
    References
    • https://pubmed.ncbi.nlm.nih.gov/40537033/
    • https://pubmed.ncbi.nlm.nih.gov/31992583/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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