Home Primary Antibodies GPX1/2 Antibody [L20K22]

research use only

GPX1/2 Antibody [L20K22]

Catalog No.: F4438

    Application: Reactivity:

    Usage Information

    Dilution
    1:100-1:1000
    1:50-1:500
    1:50-1:500
    Application
    WB, IP, IHC, IF, ELISA
    Reactivity
    Human, Mouse, Rat
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    23 kDa, 92 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    GPX1/2 Antibody [L20K22] detects endogenous levels of total GPX1/2 protein.
    Clone
    L20K22
    Synonym(s)
    Glutathione peroxidase 1; GPx-1; GSHPx-1; Cellular glutathione peroxidase; Phospholipid-hydroperoxide glutathione peroxidase GPX1; GPX1; Glutathione peroxidase 2; GPx-2; GSHPx-2; Gastrointestinal glutathione peroxidase; GPX2
    Background
    Glutathione peroxidases GPx1 and GPx2 are essential antioxidant enzymes that protect cells from oxidative damage by catalyzing the reduction of hydrogen peroxide and lipid hydroperoxides to water and corresponding alcohols, thereby preventing lipid peroxidation of cell membranes. Both enzymes contain a selenocysteine residue at their active site, encoded by the UGA stop codon, which is critical for their catalytic activity. GPx1 is ubiquitously expressed and is particularly important for protecting vascular and neural cells from oxidative stress, while GPx2 is mainly found in the gastrointestinal tract and lungs, with its expression regulated by the Nrf2 transcription factor in response to oxidative stress. GPx1 and GPx2 function as homodimers composed of alpha-helices and beta-sheets. Their catalytic mechanism involves the oxidation of the selenol group of selenocysteine by peroxides, followed by reduction by glutathione, which restores the active enzyme. Dysfunction of GPx1 or GPx2 is associated with increased risk of cardiovascular diseases, neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease, cancer, and heavy metal toxicity.
    References
    • https://pubmed.ncbi.nlm.nih.gov/21087145/
    • https://pubmed.ncbi.nlm.nih.gov/35691509/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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