E.coli LPS Antibody [M15H2] | Primary Antibodies

Application: Reactivity: Escherichia coli

Usage Information

Dilution
IF1:200

Application
IF, ELISA

Reactivity
Escherichia coli

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Positive Control	Mouse hepatocytes
Negative Control

Datasheet & SDS

Biological Description

Specificity
E.coli LPS Antibody [M15H2] specifically detects E.coli LPS.

Clone
M15H2

Synonym(s)
Escherichia coli LPS

Background
E. coli LPS (Lipopolysaccharide) constitutes the primary structural component and potent endotoxin of the Gram-negative bacterial outer membrane, comprising Lipid A, core oligosaccharide, and O-antigen that collectively maintain membrane integrity while serving as the most powerful immunostimulatory molecule known. Lipid A anchors as a bis-phosphorylated β-1',6-glucosamine disaccharide acylated with six fatty acid chains forming the toxic moiety recognized by host TLR4-MD-2-CD14 receptor complex, while the core region links via Kdo-heptose sugars bearing charged phosphate/ethanolamine groups for membrane stabilization, and the highly variable O-antigen polysaccharide chain of repeating epitopes confers serotype specificity and immune evasion through phagocytosis/complement resistance creating "smooth" bacterial morphology. LPS forms an essential permeability barrier protecting against bile salts, antibiotics, and cationic peptides while triggering catastrophic innate immune activation through MyD88/TRIF-dependent NF-κB and IRF3 pathways driving massive TNF-α, IL-1β, IL-6 cytokine storms that precipitate septic shock, disseminated intravascular coagulation, and multi-organ failure during Gram-negative bacteremia; chronic low-grade endotoxemia from gut translocation contributes to atherosclerosis, neurodegenerative diseases, and metabolic syndrome via sustained vascular inflammation, positioning LPS as both indispensable bacterial structural element and humanity's most dangerous microbial toxin.

Background

E. coli LPS (Lipopolysaccharide) constitutes the primary structural component and potent endotoxin of the Gram-negative bacterial outer membrane, comprising Lipid A, core oligosaccharide, and O-antigen that collectively maintain membrane integrity while serving as the most powerful immunostimulatory molecule known. Lipid A anchors as a bis-phosphorylated β-1',6-glucosamine disaccharide acylated with six fatty acid chains forming the toxic moiety recognized by host TLR4-MD-2-CD14 receptor complex, while the core region links via Kdo-heptose sugars bearing charged phosphate/ethanolamine groups for membrane stabilization, and the highly variable O-antigen polysaccharide chain of repeating epitopes confers serotype specificity and immune evasion through phagocytosis/complement resistance creating "smooth" bacterial morphology. LPS forms an essential permeability barrier protecting against bile salts, antibiotics, and cationic peptides while triggering catastrophic innate immune activation through MyD88/TRIF-dependent NF-κB and IRF3 pathways driving massive TNF-α, IL-1β, IL-6 cytokine storms that precipitate septic shock, disseminated intravascular coagulation, and multi-organ failure during Gram-negative bacteremia; chronic low-grade endotoxemia from gut translocation contributes to atherosclerosis, neurodegenerative diseases, and metabolic syndrome via sustained vascular inflammation, positioning LPS as both indispensable bacterial structural element and humanity's most dangerous microbial toxin.

References
https://pubmed.ncbi.nlm.nih.gov/23372159/ https://pubmed.ncbi.nlm.nih.gov/30066669/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%