CNBP | Primary Antibodies

Application: Reactivity: Mouse, Rat, Human

Lane 1: LNCAP, Lane 2: Hela, Lane 3: Jurkat, Lane 4: NIH/3T3

Usage Information

Dilution
WB1:5000-1:50000

Application
WB, IP

Reactivity
Mouse, Rat, Human

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
19 kDa 19-25 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	LNCaP cells; HeLa cells; HepG2 cells; Jurkat cells; K-562 cells; HSC-T6 cells; PC-12 cells; NIH/3T3 cells; Neuro-2a cells; HEK-293 cells; HT-29 cells; 4T1 cells
Negative Control

Datasheet & SDS

Biological Description

Specificity
CNBP Antibody [E10D2] detects endogenous levels of total CNBP protein.

Clone
E10D2

Synonym(s)
CNBP; CCHC-type zinc finger nucleic acid binding protein; Cellular nucleic acid-binding protein (CNBP); Zinc finger protein 9; RNF163; ZNF9

Background
CNBP (ZNF9) is a highly conserved CCHC-type zinc finger nucleic acid-binding protein critical for embryonic development and post-transcriptional gene regulation, characterized by seven tandem zinc knuckle motifs that coordinate zinc ions to form compact ββα folds, enabling high-affinity binding to G-rich single-stranded DNA and RNA sequences. The presence of an RGG box between the first and second zinc fingers facilitates the formation of phase-separated biomolecular droplets and enhances cooperative nucleic acid remodeling. CNBP functions as a G-quadruplex (G4) chaperone, actively unfolding stable G4 structures within the c-Myc promoter to promote RNA polymerase II transcription, and within the 5'UTR of ODC mRNA to facilitate cap-independent translation. CNBP can transactivate genes such as β-catenin and IL-6 through direct, G4-dependent promoter binding, with its activity and localization dynamically regulated by phosphorylation-triggered dimerization and nuclear translocation in response to cellular stress and cytokine signaling. Expansion of (CCTG)n repeats in CNBP intron 1 underlies myotonic dystrophy type 2 (DM2), where mutant RNA transcripts sequester functional CNBP, resulting in muscleblind-like protein toxicity, impaired myogenesis due to myf5 G4 stabilization, and progressive multisystem atrophy

Background

CNBP (ZNF9) is a highly conserved CCHC-type zinc finger nucleic acid-binding protein critical for embryonic development and post-transcriptional gene regulation, characterized by seven tandem zinc knuckle motifs that coordinate zinc ions to form compact ββα folds, enabling high-affinity binding to G-rich single-stranded DNA and RNA sequences. The presence of an RGG box between the first and second zinc fingers facilitates the formation of phase-separated biomolecular droplets and enhances cooperative nucleic acid remodeling. CNBP functions as a G-quadruplex (G4) chaperone, actively unfolding stable G4 structures within the c-Myc promoter to promote RNA polymerase II transcription, and within the 5'UTR of ODC mRNA to facilitate cap-independent translation. CNBP can transactivate genes such as β-catenin and IL-6 through direct, G4-dependent promoter binding, with its activity and localization dynamically regulated by phosphorylation-triggered dimerization and nuclear translocation in response to cellular stress and cytokine signaling. Expansion of (CCTG)n repeats in CNBP intron 1 underlies myotonic dystrophy type 2 (DM2), where mutant RNA transcripts sequester functional CNBP, resulting in muscleblind-like protein toxicity, impaired myogenesis due to myf5 G4 stabilization, and progressive multisystem atrophy

References
https://pubmed.ncbi.nlm.nih.gov/31219592/ https://pubmed.ncbi.nlm.nih.gov/34474118/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%

CNBP Antibody [E10D2]

Usage Information

Datasheet & SDS

Biological Description

Tech Support