CD163L1 Antibody [C10D1] | Primary Antibodies

Application: Reactivity: Human

Usage Information

Dilution
IHC1:2000

Application
IHC

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Positive Control	Tonsil macrophages tissue; Human spleen tissue; Human stomach tissue; Lung macrophages tissue; Human uterus tissue; Stomach macrophages tissue; Liver macrophages tissue
Negative Control	Placenta tissue

Datasheet & SDS

Biological Description

Specificity
CD163L1 Antibody [C10D1] detects endogenous levels of total CD163L1 protein.

Clone
C10D1

Synonym(s)
CD163b; CD163B; M160; UNQ6434/PRO23202; CD163L1; Scavenger receptor cysteine-rich type 1 protein M160; CD163 antigen-like 1

Background
CD163L1, also known as CD163 molecule-like 1 or M160, is a type I membrane protein of the group B scavenger receptor cysteine-rich (SRCR) superfamily that features multiple SRCR domains (typically 9–12), a single transmembrane segment, and a cytoplasmic tail that mediates endocytosis via a clathrin-dependent pathway independent of ligand cross-linking. Arising from a late evolutionary duplication of the CD163 gene, CD163L1 is highly expressed on specific tissue macrophage subsets (often colocalizing with CD163), but is minimally present or absent in monocytes, alveolar macrophages, glia, and Kupffer cells; two cytoplasmic splice variants further influence its subcellular localization. CD163L1 serves as an endocytic scavenger receptor likely binding as-yet unidentified ligands to mediate clearance and promote resolution of inflammation through anti-inflammatory signaling and maintenance of tissue homeostasis, differentiating it from CD163, which specifically scavenges hemoglobin–haptoglobin complexes. CD163L1 shows no affinity for hemoglobin–haptoglobin or tested bacteria, and does not form known protein complexes. Its regulated expression during monocyte-to-macrophage differentiation supports innate immune modulation, with disease relevance in inflammatory conditions such as atherosclerosis, potentially through oxidant clearance akin to CD163, and in autoimmune contexts by dampening excessive immune responses.

Background

CD163L1, also known as CD163 molecule-like 1 or M160, is a type I membrane protein of the group B scavenger receptor cysteine-rich (SRCR) superfamily that features multiple SRCR domains (typically 9–12), a single transmembrane segment, and a cytoplasmic tail that mediates endocytosis via a clathrin-dependent pathway independent of ligand cross-linking. Arising from a late evolutionary duplication of the CD163 gene, CD163L1 is highly expressed on specific tissue macrophage subsets (often colocalizing with CD163), but is minimally present or absent in monocytes, alveolar macrophages, glia, and Kupffer cells; two cytoplasmic splice variants further influence its subcellular localization. CD163L1 serves as an endocytic scavenger receptor likely binding as-yet unidentified ligands to mediate clearance and promote resolution of inflammation through anti-inflammatory signaling and maintenance of tissue homeostasis, differentiating it from CD163, which specifically scavenges hemoglobin–haptoglobin complexes. CD163L1 shows no affinity for hemoglobin–haptoglobin or tested bacteria, and does not form known protein complexes. Its regulated expression during monocyte-to-macrophage differentiation supports innate immune modulation, with disease relevance in inflammatory conditions such as atherosclerosis, potentially through oxidant clearance akin to CD163, and in autoimmune contexts by dampening excessive immune responses.

References
https://pubmed.ncbi.nlm.nih.gov/22900885/ https://pubmed.ncbi.nlm.nih.gov/22279103/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%