Home Primary Antibodies ATPIF1 Antibody [P8F22]

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ATPIF1 Antibody [P8F22]

Catalog No.: F4093

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:100
    1:1600
    1:3200
    Application
    WB, IP, IHC, IF
    Reactivity
    Human, Monkey
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    12 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    ATPIF1 Antibody [P8F22] detects endogenous levels of total ATPIF1 protein.
    Clone
    P8F22
    Synonym(s)
    ATPase inhibitor, mitochondrial; ATP synthase F1 subunit epsilon; Inhibitor of F(1)F(o)-ATPase (IF(1); IF1); ATP5IF1; ATPI; ATPIF1
    Background
    ATPIF1 (ATPase Inhibitory Factor 1, also IF1) is a small mitochondrial regulatory protein that physiologically inhibits the H+-ATP synthase (Complex V) to prevent ATP hydrolysis under conditions of impaired respiration or acidosis. ATPIF1 exists as an intrinsically disordered monomer in solution that undergoes disorder-to-order transition upon binding, forming two α-helices: an N-terminal helix inserting into the catalytic interface between αDP/βDP subunits of the F1 domain to sterically block rotary catalysis, and a C-terminal anti-parallel coiled-coil stabilizing IF1 homodimers. ATPIF1 binds pH-dependently to the F1 sector during ATP hydrolysis (not synthesis), locking the enzyme in the DP:IF1 conformation to preserve cellular ATP while promoting ATP synthase oligomerization (dimers/tetramers) that shapes cristae architecture and inhibits permeability transition pore (PTP) opening via OSCP subunit interaction. It triggers retrograde signaling, inducing aerobic glycolysis (Warburg effect) in cancer cells and modulates mitohormesis/apoptosis resistance. Overexpression in carcinomas drives oncogenesis, metabolic reprogramming, and tumor survival; deficiency disrupts cristae/mitochondrial structure, linking to pathologies like heart failure.
    References
    • https://pubmed.ncbi.nlm.nih.gov/35620611/
    • https://pubmed.ncbi.nlm.nih.gov/38280428/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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