Home Primary Antibodies Acetyl-CoA Carboxylase 2 Antibody [E20M20]

research use only

Acetyl-CoA Carboxylase 2 Antibody [E20M20]

Catalog No.: F3039

    Application: Reactivity:
    • F3039-wb
      Lane 1: Human adipocytes

    Usage Information

    Dilution
    1:1000
    1:50
    Application
    WB, IP
    Reactivity
    Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    280 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    Acetyl-CoA Carboxylase 2 Antibody [E20M20] detects endogenous levels of total Acetyl-CoA Carboxylase 2 protein.
    Clone
    E20M20
    Synonym(s)
    Acetyl-CoA carboxylase 2; ACC-beta; ACACB; ACC2; ACCB
    Background
    Acetyl-CoA Carboxylase 2 (ACC2) is a biotin-dependent carboxylase enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, primarily regulating fatty acid oxidation in oxidative tissues like heart and skeletal muscle. ACC2 features a multi-domain architecture including a biotin carboxylase (BC) domain that carboxylates biotin using ATP and bicarbonate, a biotin carboxyl carrier protein (BCCP) domain that shuttles the carboxyl group, and a carboxyltransferase (CT) domain that transfers it to acetyl-CoA. A unique N-terminal hydrophobic sequence targets and anchors ACC2 to the outer mitochondrial membrane, positioning it near carnitine palmitoyltransferase 1 (CPT1). ACC2 operates as a dimer or filament in its active form, with activity modulated by phosphorylation. Mitochondrially localized malonyl-CoA produced by ACC2 allosterically inhibits CPT1, preventing long-chain fatty acid entry into mitochondria for beta-oxidation during energy-replete states. This mechanism maintains metabolic balance by suppressing fat breakdown when synthesis predominates, with ACC2 knockout enhancing oxidation and insulin sensitivity. ACC2 dysregulation contributes to metabolic disorders, including obesity, type 2 diabetes, and cardiovascular disease.
    References
    • https://pubmed.ncbi.nlm.nih.gov/10677481/
    • https://pubmed.ncbi.nlm.nih.gov/35359351/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

    * Indicates a Required Field

    Please enter your name.
    Please enter your email. Please enter a valid email address.
    Please write something to us.