Home Primary Antibodies Acetyl-α-Tubulin (Lys40) Antibody [F22D16]

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Acetyl-α-Tubulin (Lys40) Antibody [F22D16]

Catalog No.: F2686

    Application: Reactivity:

    Usage Information

    Dilution
    1:15000-1:30000
    Application
    WB, IHC
    Reactivity
    bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years

    Datasheet & SDS

    Biological Description

    Specificity
    Acetyl-α-Tubulin (Lys40) Antibody [F22D16] detects endogenous levels of total α-Tubulin protein only when it is Acetylated at Lys40.
    Clone
    F22D16
    Synonym(s)
    Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain; TUBA4A; TUBA1
    Background
    Acetyl-α-tubulin (Lys40) represents the lysine 40-acetylated form of α-tubulin, a core subunit of the tubulin heterodimer family that polymerizes into microtubules, essential cytoskeletal elements in eukaryotic cells. Acetylation targets the ε-amino group of the highly conserved Lys40 residue within a flexible 20-amino-acid luminal loop (residues 37-56) that protrudes into the microtubule interior, where it folds into an irregular turn packing against the acetyltransferase TAT active site funnel formed by conserved residues like Gln58, Arg158, Ile64, and Asp157 for substrate recognition and deprotonation. This internal modification, unique among microtubule PTMs, occurs post-polymerization via TAT accessing the lumen through lattice openings. Lys40 acetylation enhances microtubule flexibility and resilience to mechanical stress by altering protofilament rotational angles and lattice stability, promoting long-lived microtubules critical for dendritic arborization, axon growth, intracellular transport, cell motility, and neuronal branching. It is dynamically reversed by HDAC6 deacetylation, integrating into mechanosensitive pathways where stable acetylated MTs support ciliary/flagellar beating and polarity establishment. Dysregulation links to neurodegeneration, as reduced acetylation impairs dendritic stability and locomotion in models like Drosophila atat mutants. .
    References
    • https://pubmed.ncbi.nlm.nih.gov/36827311/
    • https://pubmed.ncbi.nlm.nih.gov/24906155/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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