Molecular Weight(MW): 413.49
NU7441 (KU-57788) is a highly potent and selective DNA-PK inhibitor with IC50 of 14 nM and also inhibits PI3K with IC50 of 5 μM in cell-free assays.
Cited by 16 Publications
5 Customer Reviews
(C) Microirradiation live-cell microscopy of U2OS cells stably expressing mEGFP-MacroD2 full-length construct and treated with KU55933 10 μM, VE-821 1 μM, NU7441 1 μM or DMSO. Scale bar, 10 μm.
Nucleic Acids Res, 2017, 45(1):244-254. NU7441 (KU-57788) purchased from Selleck.
Cells were treated with cisplatin under the following conditions: pretreatment with Nu7026 (Nu7026, 26) or Nu7441 (Nu7441, 41). Before DNA damage, wortmannin was applied at 100 nM for 30 min, Nu7026 at 10 uM for 1 h, Nu7441 at 1 uM for 1 h. Accompanying quantifications of the adjusted nucleolar EU fluorescence are shown. Each condition was normalized to cells treated with DMSO or indicated inhibitors without DNA damaging treatment. Two-way ANOVA was followed by Holm-Sidak's test for the comparison of cells with and without inhibitors. *, **, *** represent P ≤ 0.05, 0.01, 0.001, respectively.
Nucleic Acids Res 2013 41(15), 7378-86. NU7441 (KU-57788) purchased from Selleck.
Confocal immunostaining of S2056 phosphorylation of DNA-PKcs following radiation in HEK 293T and p18CycE-expressing cells.
Nucleic Acids Res 2013 41, 10157-69. NU7441 (KU-57788) purchased from Selleck.
Validation of activity and specificity of chemical inhibitors of; ATM, ATR, and DNAPK. H460 cells were treated with 1 uM camptothecin (CPT) or 20 ug/ml bleomycin for 1 h in the presence of the indicated inhibitors: DNAPK-i1—NU7026, DNAPK-i2—NU7441. MSH6, fibrillarin, and tubulin were used as loading controls.Effects of DNAPK inhibitors on its autophosphorylation in bleomycin-treated cells.
Toxicol Sci 2014 10.1093/toxsci/kfu207. NU7441 (KU-57788) purchased from Selleck.
Purity & Quality Control
Choose Selective DNA-PK Inhibitors
|Description||NU7441 (KU-57788) is a highly potent and selective DNA-PK inhibitor with IC50 of 14 nM and also inhibits PI3K with IC50 of 5 μM in cell-free assays.|
NU7441 increases the persistence of γH2AX foci after ionizing radiation–induced or etoposide-induced DNA damage. NU7441 (0.5 μM or 1 μM) appreciably increases G2-M accumulation induced by ionizing radiation, etoposide, and doxorubicin in both SW620 and LoVo cells.  NU7441 causes persistence of doxorubicin- and ionising radiation-induced DNA double-strand break and also slightly decreases homologous recombination activity DNA-PK-proficient M059-Fus-1 and DNA-PK-deficient M059 J human tumour cells.  NU7441 inhibits UV-induced RPA p34 hyperphosphorylation in a dose-dependent manner both in cells lacking and cells expressing polymerase η.  NU7441 increases levels of fludarabine-induced γH2AX foci and correspondingly decreased fludarabine-induced cell death in chronic lymphocytic leukemia cells.  NU7441 also inhibits mitoxantrone-induced DNA-PKcs autophosphorylation and repair in chronic lymphocytic leukemia cells. 
|In vivo||NU7441 intraperitoneally administrated at dose of 10 mg/kg maintains for at least 4 hours shows nontoxic and increases etoposide-induced tumor growth delay 2-fold in mice bearing SW620 xenografts. |
-  Leahy JJ, et al. Bioorg Med Chem Lett, 2004, 14(24), 6083-6087.
-  Zhao Y, et al. Cancer Res, 2006 , 66(10), 5354-5362.
-  Tavecchio M, et al. Cancer Chemother Pharmacol, 2012, 69(1), 155-164.
|In vitro||DMSO||4 mg/mL warmed (9.67 mM)|
|In vivo||Add solvents individually and in order:
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
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Frequently Asked Questions
Previous aliquots were clear and colorless while this time, it becomes yellow when the powder was dissolved in DMSO? Could you advice on this?
Slight color variation due to different processing is normal and usually doesn't interfere with the function.