Home Microbiology β-lactamase inhibitor Nafcillin Sodium

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Nafcillin Sodium β-lactamase inhibitor

Cat.No.S4042

Nafcillin sodium (CL 8491) reversibly inhibits β-lactamase with Kd of 33 mM.
Nafcillin Sodium β-lactamase inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 454.47

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Quality Control

Batch: S404201 DMSO]91 mg/mL]false]Water]91 mg/mL]false]Ethanol]20 mg/mL]false Purity: 99.10%
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99.10

Solubility

In vitro
Batch:

DMSO : 91 mg/mL (200.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 91 mg/mL

Ethanol : 20 mg/mL

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In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Chemical Information, Storage & Stability

Molecular Weight 454.47 Formula

C21H21N2O5S.H2O.Na

 Storage (From the date of receipt)
CAS No. 7177-50-6 Download SDF Storage of Stock Solutions

Synonyms CL 8491 Smiles CCOC1=C(C2=CC=CC=C2C=C1)C(=O)NC3C4N(C3=O)C(C(S4)(C)C)C(=O)[O-].O.[Na+]

Mechanism of Action

Targets/IC50/Ki
β-lactamase
33 mM(Kd)
In vitro
Nafcillin sodium inactivates β-lactamase through forming a covalent acyl-enzyme intermediate with β-lactamase via Ser-70. At concentrations above 80 mM, Nafcillin displays complete inhibition of β-lactamase within 200 s. Upon removal of the excess Nafcillin, the inhibited enzyme can be re-activated completely, with a rate constant of 2×10-3/s (25 ℃). Nafcillin sodium is activity against penicillin-resistant staphylococci. The minimal concentration necessary for antistaphylococcic activity in vitro ranges from 0.2 μg/mL to 2 μg/mL.
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