Home Stem Cells & Wnt Notch inhibitor FLI-06

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FLI-06 Notch inhibitor

Cat.No.S7399

FLI-06 is a novel inhibitor of Notch signaling with EC50 of 2.3 μM.
FLI-06 Notch inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 438.52

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: S739901 DMSO]88 mg/mL]false]Ethanol]8 mg/mL]false]Water]Insoluble]false Purity: 99.9%
99.9

Cell Culture, Treatment & Working Concentration

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-231 cells Cytotoxicity assay Cytotoxicity against human MDA-MB-231 cells, IC50=4 μM
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight 438.52 Formula

C25H30N2O5

 Storage (From the date of receipt)
CAS No. 313967-18-9 Download SDF Storage of Stock Solutions

Synonyms N/A Smiles CC1=C(C(C2=C(N1)CC(CC2=O)(C)C)C3=CC=C(C=C3)[N+](=O)[O-])C(=O)OC4CCCCC4

Solubility

In vitro
Batch:

DMSO : 88 mg/mL (200.67 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 8 mg/mL

Water : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
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In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Mechanism of Action

Targets/IC50/Ki
Notch [1]
(Cell-based assay)
2.3 μM(EC50)
In vitro
In HeLa NotchΔE-eGFP cells, FLI-06 blocks Notch trafficking and processing. This compound changes the maturation pattern of APP and abolishes the shedding of APPs in HEK293 cells stably expressing a mutated APP that yields robust amounts of amyloid β. It disrupts the Golgi apparatus, and inhibits general secretion at a step before exit from the endoplasmic reticulum (ER), which is accompanied by a tubule-to-sheet morphological transition of the ER. [1]
In vivo
FLI-06 (50 μM) inhibits endogenous Notch signaling in zebrafish embryos. [1]
References

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