Catalog No.S7379

E-64 is an irreversible and selective cysteine protease inhibitor with IC50 of 9 nM for papain.

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E-64 Chemical Structure

E-64 Chemical Structure
Molecular Weight: 357.41

Validation & Quality Control

Quality Control & MSDS

Product Information

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Product Description

Biological Activity

Description E-64 is an irreversible and selective cysteine protease inhibitor with IC50 of 9 nM for papain.
Targets Cysteine protease [1]
IC50 9 nM
In vitro E-64 rapidly inhibits the activities of the cysteine proteinases cathepsins B, H and L and papain, while has no effect on the serine proteinases or the metalloproteinases. [2] E-64, as a specific inhibitor os cysteine proteases, effectively blocks in vitro ovarian cancer cell invasion. [3] In addition, E-64 also shows antiparasitic activity against Giardia lamblia excystation in vitro. [4] E-64 improves the preimplantation development of bovine somatic cell nuclear transfer embryos, which indicates another important implication of E-64. [5]
In vivo E-64 induces a marked decrease in the number of spontaneous metastasis in M5076 tumor bearing mice, while has no effect on the formation of experimental metastasis. [6] E-64 also shows antiparasitic activity against Giardia lamblia excystation in infected mice. [4]

Protocol(Only for Reference)

Animal Study: [5]

Animal Models M5076 tumor bearing mice
Administration i.v.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)
Body Surface Area (m2)0.0070.0250.
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)


[1] Matsumoto K, et al. Biopolymers. 1999, 51(1), 99-107.

[2] Barrett AJ, et al. Biochem J. 1982, 201(1), 189-198.

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Chemical Information

Download E-64 SDF
Molecular Weight (MW) 357.41


CAS No. 66701-25-5
Storage 3 years -20℃powder
2 years -80℃in solvent
Synonyms N/A
Solubility (25°C) * In vitro DMSO 71 mg/mL warming (198.65 mM)
Water 11 mg/mL warming (30.77 mM)
Ethanol <1 mg/mL (<1 mM)
In vivo
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name 3-​[[[(1S)-​1-​[[[4-​[(aminoiminomethyl)amino]butyl]amino]carbonyl]-​3-​methylbutyl]amino]carbonyl]-​(2S,​3S)-​oxiranecarboxylic acid

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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