BI 2536

Catalog No.S1109

BI2536 is a potent Plk1 inhibitor with IC50 of 0.83 nM in a cell-free assay. It shows 4- and 11-fold greater selectivity against Plk2 and Plk3. Phase 2.

Price Stock Quantity  
USD 240 In stock
USD 120 In stock
USD 210 In stock
USD 670 In stock
USD 997 In stock
Bulk Inquiry

Massive Discount Available

Free Overnight Delivery on all orders over $ 500.

BI 2536 Chemical Structure

BI 2536 Chemical Structure
Molecular Weight: 521.66

Validation & Quality Control

Cited by 52 publications:

10 customer reviews :

Quality Control & MSDS

Related Compound Libraries

PLK Inhibitors with Unique Features

  • Selective Plk Inhibitors

    GSK461364 Plk1-selective, Ki=2.2 nM.

  • Plk Inhibitor in Clinical Trial

    Rigosertib (ON-01910) Phase III for Myelo Dysplastic Syndrome (MDS).

  • Newest Plk Inhibitor

    Ro3280 Potent, highly selective inhibitor of Polo-like kinase 1 (PLK1) with IC50 of 3 nM.

  • Calssic Plk Inhibitor

    Volasertib (BI 6727) Highly potent Plk1 inhibitor with IC50 of 0.87 nM and in phase II trials.

Product Information

  • Compare PLK Inhibitors
    Compare PLK Products
  • Research Area
  • Inhibition Profile
  • BI 2536 Mechanism

Product Description

Biological Activity

Description BI2536 is a potent Plk1 inhibitor with IC50 of 0.83 nM in a cell-free assay. It shows 4- and 11-fold greater selectivity against Plk2 and Plk3. Phase 2.
Targets PLK1 [1]
(Cell-free assay)
PLK2 [1]
(Cell-free assay)
PLK3 [1]
(Cell-free assay)
PI3Kα [1]
(Cell-free assay)

 View  More

IC50 0.83 nM 3.5 nM 9.0 nM 2.407 μM
In vitro BI 2536 blocks the activities of Plk2 and Plk3 to a slightly lesser extent with IC50 of 3.5 nM and 9.0 nM, respectively. In HeLa cells, BI 2536 treatment ranging from 10-100 nM leads to the blocking of the recruitment of γ-tubulin and phosphorylation of Apc6 at mitotic centrosomes, inhibition of cohesin release from chromosome arms, induction of monopolar spindles, as well as a range of other mitotic processes that are known to depend on Plk1. BI 2536 treatment leads to the HeLa cells arrested in G2/M, subsequently a sub-G1 DNA peak indicative of DNA breakdown and apoptosis, and accumulated cleaved PARP p85 fragments in a concentration-dependent manner. BI 2536 inhibits the growth of a panel of 32 human cancer cell lines with EC50 of 2-25 nM, while blocking the proliferation of exponentially growing hTERT-RPE1, human umbilical vein endothelial cells (HUVECs), and normal rat kidney (NRK) cells with EC50 of 12-31 nM. [1] Plk1 inhibition by BI 2536 reduces the growth and viability of anaplastic thyroid carcinoma (ATC) cells such as CAL62, OCUT-1, SW1736, 8505C, and ACT-1 with EC50 values of 1.4-5.6 nM. [2]
Cell Data
Cell LinesAssay TypeConcentrationIncubation TimeFormulationActivity DescriptionPMID
PSN1NH7qZoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M3fqcmlEPTB;MD6wNFc4PiEQvF2=MX;TRW5ITVJ?
ACNMYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MlTMTWM2OD1yLkCwPFQ{KM7:TR?=MlHXV2FPT0WU
TE-8NILmPZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NVXnSIUxUUN3ME2wMlAxQTRzIN88US=>MYnTRW5ITVJ?
LC-2-adM3r2[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NXGyNohJUUN3ME2wMlAyODl|IN88US=>NHG4XXdUSU6JRWK=
ALL-PONVnsRmhDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NUTQXlU2UUN3ME2wMlAyOyEQvF2=NGjEd4VUSU6JRWK=
EW-16M1ruVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NGC4OY9KSzVyPUCuNFE{OzZizszNMWnTRW5ITVJ?
KM12NV3kdIg3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NFLadWdKSzVyPUCuNFE{PDhizszNNHLVfXZUSU6JRWK=
GI-1M3z0OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MUfJR|UxRTBwMEGzPFMh|ryPNVLKXVRCW0GQR1XS
LS-411NMVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NXS2SFBWUUN3ME2wMlAyPDB5IN88US=>NWmzUJZCW0GQR1XS
ES8M4HCWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7Mk\NTWM2OD1yLkCxOFk1KM7:TR?=NUDYV2xmW0GQR1XS
SNU-C2BM{O0bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NXzKXm9pUUN3ME2wMlAyPTJ4IN88US=>MUfTRW5ITVJ?
CESSM3;WUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MX\JR|UxRTBwMEG2NFIh|ryPMn;UV2FPT0WU
697M2fNNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M32wcGlEPTB;MD6wNVY3QCEQvF2=M2j4Z3NCVkeHUh?=
NCI-H1963NIOzPXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=Mlr0TWM2OD1yLkCxOlc{KM7:TR?=MUTTRW5ITVJ?
IA-LMNFjVe3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NVHBXGtPUUN3ME2wMlAyPjh6IN88US=>NV31PVJEW0GQR1XS
SW954MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MVfJR|UxRTBwMEG3NFUh|ryPMn3HV2FPT0WU
RL95-2NXL0fYZ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MmfrTWM2OD1yLkCxO|M5KM7:TR?=M{\V[HNCVkeHUh?=
LXF-289MmS0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M4jZdWlEPTB;MD6wNVc4KM7:TR?=NF3SUZJUSU6JRWK=
OCUB-MM{DLcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NYP0SlZNUUN3ME2wMlAyQDJ4IN88US=>NWDZcWNKW0GQR1XS
RKONEHv[JpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MUjJR|UxRTBwMEG5PFch|ryPM{LtOnNCVkeHUh?=
LOUCYMWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NGHQR21KSzVyPUCuNFIxPSEQvF2=NIfkZXJUSU6JRWK=
NOS-1NHf1WIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MXXJR|UxRTBwMEKwPVMh|ryPM{LwenNCVkeHUh?=
A4-FukMornS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MXHJR|UxRTBwMEKyNVgh|ryPMo\3V2FPT0WU
A388M3W3S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MmPETWM2OD1yLkCyNlI4KM7:TR?=MV;TRW5ITVJ?
TE-9MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NEL4UIlKSzVyPUCuNFIzPjFizszNM1zPOHNCVkeHUh?=
KURAMOCHINIe3[YlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NGrWRYhKSzVyPUCuNFIzQDVizszNM2rocXNCVkeHUh?=
KALS-1MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NVrmTXN3UUN3ME2wMlAzOzN6IN88US=>MX7TRW5ITVJ?
HOP-62MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M2TKTmlEPTB;MD6wNlQzKM7:TR?=M3P2RnNCVkeHUh?=
RPMI-8402MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NFvCc4RKSzVyPUCuNFI2ODhizszNMo\RV2FPT0WU
TE-1NGXBPGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NXn1eJZJUUN3ME2wMlAzPTF{IN88US=>NHe2eoZUSU6JRWK=
CCRF-CEMMlPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M4GxV2lEPTB;MD6wNlU1OyEQvF2=M3P6NXNCVkeHUh?=
HUTU-80NXXmTGF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MVfJR|UxRTBwMEK2NlQh|ryPM2XBWXNCVkeHUh?=
NCI-H1648MkftS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M4DNcGlEPTB;MD6wNlY1PyEQvF2=NIDHSoxUSU6JRWK=
HCE-4MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M{flTGlEPTB;MD6wNlY5OiEQvF2=NXT4dGUxW0GQR1XS
ETK-1NFzzZY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M2K1cWlEPTB;MD6wNlc{PiEQvF2=NE\iNlZUSU6JRWK=
TE-15NVXKUpRxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NGDNUXVKSzVyPUCuNFI4OzhizszNNIfBfndUSU6JRWK=
BE-13MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NXLXcIVbUUN3ME2wMlAzPzRizszNM13E[3NCVkeHUh?=
KE-37NFvu[lBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MlfwTWM2OD1yLkCyO|QyKM7:TR?=MVrTRW5ITVJ?
NCI-H1355NV;UZWtZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NFrmSHBKSzVyPUCuNFI4QDNizszNM3O1ZXNCVkeHUh?=
TE-11MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MWXJR|UxRTBwMEK4NFkh|ryPM3myTHNCVkeHUh?=
BL-70MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M2PrVGlEPTB;MD6wNlg2PyEQvF2=NFvSe3NUSU6JRWK=
ES6MnjTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MWnJR|UxRTBwMEK4PFEh|ryPNFHrNYFUSU6JRWK=
GT3TKBMlGwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MYfJR|UxRTBwMEK5OVMh|ryPMV7TRW5ITVJ?
LB647-SCLCNEXEd5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MYPJR|UxRTBwMEOxPVUh|ryPM2DJfnNCVkeHUh?=
NB13NH:0[ZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MVPJR|UxRTBwMEOzNVYh|ryPNUXSO|FFW0GQR1XS
ST486MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MljMTWM2OD1yLkCzN|M4KM7:TR?=M4W5V3NCVkeHUh?=
NCI-H82MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NXn5ZotlUUN3ME2wMlA{OzR|IN88US=>NX\zSIMzW0GQR1XS
NTERA-S-cl-D1MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NEXhWZZKSzVyPUCuNFM{QTlizszNMWfTRW5ITVJ?
ES4NV32T4VtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M1r2SGlEPTB;MD6wN|Q3OyEQvF2=M37Ed3NCVkeHUh?=
SK-NEP-1M1LuT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7NIXhdoxKSzVyPUCuNFM1QTlizszNNU\mSoI1W0GQR1XS
BeckerMWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MUfJR|UxRTBwMEO1OVUh|ryPMU\TRW5ITVJ?
A101DMkLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M3q0[mlEPTB;MD6wN|c1QCEQvF2=M1P6SnNCVkeHUh?=
SK-UT-1NVK4cZV7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NVfQNFlNUUN3ME2wMlA{Pzd2IN88US=>NF3VZ4NUSU6JRWK=
SW982MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NXjDNFZyUUN3ME2wMlA{PzlizszNMlvQV2FPT0WU
TE-10MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NVPJOJR5UUN3ME2wMlA1ODR4IN88US=>M{K0eHNCVkeHUh?=
OVCAR-4MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M3nrWmlEPTB;MD6wOFA2QSEQvF2=NIfDenRUSU6JRWK=
KP-N-YSNF63bJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NEHUR2tKSzVyPUCuNFQxQDVizszNM2DvRXNCVkeHUh?=
BL-41NUHPRll1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MU\JR|UxRTBwMESxNlUh|ryPMX;TRW5ITVJ?
MZ1-PCMWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NUXMXmd1UUN3ME2wMlA1OTJ5IN88US=>NHTjcnpUSU6JRWK=
PF-382M3jkT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MVfJR|UxRTBwMESxPVgh|ryPM1jzVXNCVkeHUh?=
MOLT-13NHzhd29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NH3rNY1KSzVyPUCuNFQzOzhizszNMY\TRW5ITVJ?
HCC1599NGH2TYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=Mo[yTWM2OD1yLkC0NlU5KM7:TR?=Mmq1V2FPT0WU
IST-MEL1M320eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NG\6RmdKSzVyPUCuNFQ1PDJizszNMmHzV2FPT0WU
LU-139NFvHc4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MWHJR|UxRTBwMES0OFIh|ryPM2TJSnNCVkeHUh?=
MS-1MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MVHJR|UxRTBwMES3NFEh|ryPNV2wcpNYW0GQR1XS
MFH-inoNUH1dIJqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=Mm[0TWM2OD1yLkC1NFA{KM7:TR?=Mmr6V2FPT0WU
IMR-5NUHVUVZ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MlO1TWM2OD1yLkC1NFIyKM7:TR?=Mme2V2FPT0WU
TE-5NVvId|VKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NG\lepZKSzVyPUCuNFUxPjNizszNM1PkSnNCVkeHUh?=
DJM-1MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NHq4XXVKSzVyPUCuNFUyPDJizszNM2jwPXNCVkeHUh?=
NB17MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NELtNoNKSzVyPUCuNFUzODdizszNMlXIV2FPT0WU
KARPAS-45NXTqcnZJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NUnOVJJzUUN3ME2wMlA2OzV6IN88US=>NGOz[opUSU6JRWK=
TGBC1TKBNV6yW3NkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M2fL[GlEPTB;MD6wOVQxPSEQvF2=MY\TRW5ITVJ?
ML-2NYLrRZp7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=Mli1TWM2OD1yLkC1OFE1KM7:TR?=MoDHV2FPT0WU
no-10M4HIb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7M{HxVmlEPTB;MD6wOVQ4PCEQvF2=NWjsT5lIW0GQR1XS
CTV-1NHHCWmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NWPQ[4dFUUN3ME2wMlA2PTB{IN88US=>M1rwenNCVkeHUh?=
SJSA-1NXLqWlM{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NH;hWnRKSzVyPUCuNFU2OiEQvF2=NV7pN2V[W0GQR1XS
HC-1M1PYdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MYXJR|UxRTBwMEW1PVch|ryPMnjKV2FPT0WU
TE-12M3TI[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MlHmTWM2OD1yLkC1OlE{KM7:TR?=MWDTRW5ITVJ?
KY821NWm1[3VpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MWrJR|UxRTBwMEW2N|Ih|ryPMYnTRW5ITVJ?
TALL-1NU\Me2pxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M3ryWGlEPTB;MD6wOVcxQCEQvF2=NVzLNpFvW0GQR1XS
DELMo\XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NUPhbFU2UUN3ME2wMlA2PzB7IN88US=>NUHoRnhzW0GQR1XS
TE-6M4[1Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MlrWTWM2OD1yLkC1O|IzKM7:TR?=M{TJeHNCVkeHUh?=
SK-LMS-1MmjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M3;ve2lEPTB;MD6wOVgxPSEQvF2=M1rpU3NCVkeHUh?=
EW-1MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MkC4TWM2OD1yLkC1PFM4KM7:TR?=Mn;vV2FPT0WU
8-MG-BANIDNV25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MV;JR|UxRTBwMEW4OVEh|ryPMWDTRW5ITVJ?
NALM-6M3\J[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NVfwWVVQUUN3ME2wMlA2QTd|IN88US=>M2XBSXNCVkeHUh?=
LB771-HNCM{LhSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NWTNR2hIUUN3ME2wMlA2QTd7IN88US=>NHHhToFUSU6JRWK=
HT-144MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NXXabYNFUUN3ME2wMlA3OTNizszNMX3TRW5ITVJ?
KM-H2NX:0dW5QT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M3TsSWlEPTB;MD6wOlIzOSEQvF2=M17jUHNCVkeHUh?=
SF268NXPhTo5yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NGDMdnZKSzVyPUCuNFYzQDZizszNM4jjdnNCVkeHUh?=
RS4-11MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MXjJR|UxRTBwME[zNFQh|ryPMnqwV2FPT0WU
MHH-PREB-1MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M3LNO2lEPTB;MD6wOlMyQSEQvF2=MY\TRW5ITVJ?
ATN-1M3roVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NYXycItOUUN3ME2wMlA3OzR3IN88US=>NYqx[HFHW0GQR1XS
BB30-HNCMVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M1mwRWlEPTB;MD6wOlQ{PiEQvF2=MnjDV2FPT0WU
CPC-NMkTQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NI\QS4hKSzVyPUCuNFY2OjRizszNNVG5e4FsW0GQR1XS
NB1Mlm3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MnTQTWM2OD1yLkC2OlE{KM7:TR?=M{L5W3NCVkeHUh?=
KS-1NUjOfIp7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NITmcFRKSzVyPUCuNFY4PDhizszNMn76V2FPT0WU
RXF393M4nmcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M2rUdmlEPTB;MD6wOlc3PCEQvF2=NHzuVlRUSU6JRWK=
IST-SL2NEO3Uo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NVPaU2JiUUN3ME2wMlA4ODB2IN88US=>M1\6PXNCVkeHUh?=
GI-ME-NNIjlSVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MlTCTWM2OD1yLkC3Nlg3KM7:TR?=MWPTRW5ITVJ?
VA-ES-BJMWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MmPsTWM2OD1yLkC3OFc2KM7:TR?=NHv0NIZUSU6JRWK=
NCI-H1581M{TvW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHPh[VhKSzVyPUCuNFc1PzhizszNNGnYOYJUSU6JRWK=
U-266NVTMdZc5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MWDJR|UxRTBwMEe0PVch|ryPM1fxeXNCVkeHUh?=
DMS-114NVrvZ3pJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MUfJR|UxRTBwMEe1Nlgh|ryPM37Se3NCVkeHUh?=
NCI-H187MljrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NYP6cHFPUUN3ME2wMlA4PzJ4IN88US=>NG[yV2ZUSU6JRWK=
SW962MofYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NG\KSG5KSzVyPUCuNFc5OzlizszNMULTRW5ITVJ?
MONO-MAC-6MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M4rX[WlEPTB;MD6wO|k5PiEQvF2=NGfxWJdUSU6JRWK=
MOLT-16NUjSTVNFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MV;JR|UxRTBwMEixNlEh|ryPMor5V2FPT0WU
KNS-42M2Tpd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MUnJR|UxRTBwMEixOlEh|ryPNIjTd|FUSU6JRWK=
CMKNHfjSFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NIrNNnhKSzVyPUCuNFgzOjVizszNM2X2ZXNCVkeHUh?=
Ramos-2G6-4C10MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MmrnTWM2OD1yLkC4NlI2KM7:TR?=M1nhR3NCVkeHUh?=
DaudiM4PCSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M2rBXWlEPTB;MD6wPFI2PiEQvF2=NULHXmdSW0GQR1XS
NCI-H510ANIHQ[IFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NXfxPWlEUUN3ME2wMlA5OzN4IN88US=>MUjTRW5ITVJ?
LB831-BLCMX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MnToTWM2OD1yLkC4OVYyKM7:TR?=MV7TRW5ITVJ?
HELMnThS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M3vFTGlEPTB;MD6wPFY3QCEQvF2=NWK1b2V7W0GQR1XS
LU-134-AM17tV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7NGnOOHhKSzVyPUCuNFg4ODhizszNMXnTRW5ITVJ?
NCI-H1694MmHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?Mn3OTWM2OD1yLkC4O|Q1KM7:TR?=NUPmWXhVW0GQR1XS
KGNNGD0cHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M{ni[GlEPTB;MD6wPFc2QSEQvF2=NESw[pdUSU6JRWK=
A253MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NHXEcpBKSzVyPUCuNFg5OjJizszNNYrlXZBCW0GQR1XS
DG-75NG[wSYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NHPwWINKSzVyPUCuNFg6OjJizszNNE\KV|BUSU6JRWK=
MOLT-4MlKyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M4nQRWlEPTB;MD6wPFk5OyEQvF2=MWHTRW5ITVJ?
NCI-SNU-5MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MmfUTWM2OD1yLkC5NlU5KM7:TR?=NEHwUYNUSU6JRWK=
ONS-76M1[2R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHL6eY1KSzVyPUCuNFkzPzZizszNM1vDSHNCVkeHUh?=
NCI-H446M3LHWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M{\kb2lEPTB;MD6wPVQ3OiEQvF2=NF\1R2JUSU6JRWK=
COR-L279MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MWHJR|UxRTBwMEm1OVYh|ryPMWLTRW5ITVJ?
Calu-6MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MYLJR|UxRTBwMEm1PVgh|ryPNYnEeHBDW0GQR1XS
BC-3MnXpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NYqydmNVUUN3ME2wMlA6PjZ{IN88US=>NVTyU|dNW0GQR1XS
NCI-H2126NUfOfFBzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NHjSO|VKSzVyPUCuNFk4QTFizszNM4D5ZnNCVkeHUh?=
NCI-H1882NXLte2xJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NYHlSoQ2UUN3ME2wMlA6QDB7IN88US=>NFzVRlRUSU6JRWK=
BB65-RCCNFzISJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MmK0TWM2OD1yLkGwNlk1KM7:TR?=Mn;sV2FPT0WU
DU-4475M{\jOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MnrLTWM2OD1yLkGwOFA4KM7:TR?=MYTTRW5ITVJ?
NCI-H526NEj4NJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NXf6RYVuUUN3ME2wMlExPDRizszNM1zXNnNCVkeHUh?=
NEC8MmTiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M4HNV2lEPTB;MD6xNFUzPSEQvF2=MUDTRW5ITVJ?
NCI-H1304M13HVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NGK5cmxKSzVyPUCuNVA2QTRizszNNIfid3VUSU6JRWK=
L-540M2Xzbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NIGwbZFKSzVyPUCuNVA4ODhizszNM2TlPHNCVkeHUh?=
NCI-SNU-16NF;LXY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M2[4N2lEPTB;MD6xNFc1OiEQvF2=Mn[xV2FPT0WU
HD-MY-ZNUXibFBGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MnjRTWM2OD1yLkGwPFU3KM7:TR?=NX\KTmhyW0GQR1XS
K-562MnXJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NX7XU5pTUUN3ME2wMlExQDV6IN88US=>NWi1O2JDW0GQR1XS
JARMXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M2jxN2lEPTB;MD6xNFkzPSEQvF2=NG\JS49USU6JRWK=
MZ2-MELMkf4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NXi0Uml1UUN3ME2wMlEyODVizszNNXe1Rm1kW0GQR1XS
L-363M1\ZbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NUDISYg1UUN3ME2wMlEyODd7IN88US=>NXrjc|dDW0GQR1XS
BV-173M2rGeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MonETWM2OD1yLkGxNlQ{KM7:TR?=MnzCV2FPT0WU
NCI-H524NWTm[YE4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NX;CXoo3UUN3ME2wMlEyOjd2IN88US=>M3WwbXNCVkeHUh?=
EoL-1-cellNX;EXZF2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MUTJR|UxRTBwMUG2O|Ih|ryPNWLaU2V2W0GQR1XS
K052MmXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M{HKZ2lEPTB;MD6xNVcyOiEQvF2=NHP3NINUSU6JRWK=
TK10NFvFWlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NET1TpdKSzVyPUCuNVE4OjhizszNMn72V2FPT0WU
KU812MknkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NX7Xb5psUUN3ME2wMlEzODR2IN88US=>M2LzNXNCVkeHUh?=
RPMI-8226MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NEjXfWNKSzVyPUCuNVIxPTJizszNNEG4fI5USU6JRWK=
MZ7-melMnSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M{T5eWlEPTB;MD6xNlI{PyEQvF2=NXu1[3RxW0GQR1XS
SK-PN-DWMUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M{n4bGlEPTB;MD6xNlI5PyEQvF2=M{jKO3NCVkeHUh?=
SRNVvzdJNiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M1zPRWlEPTB;MD6xNlQ1OSEQvF2=NHrtbGFUSU6JRWK=
RPMI-6666M2XwPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M1i5dWlEPTB;MD6xNlc1OyEQvF2=NI\NcHRUSU6JRWK=
no-11MoezS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NGrpV4JKSzVyPUCuNVI5PjlizszNNGf6VmpUSU6JRWK=
EW-3MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MmHsTWM2OD1yLkGyPFk{KM7:TR?=NFjNe2ZUSU6JRWK=
LAMA-84Mo\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MmOzTWM2OD1yLkGzNVg4KM7:TR?=NHTrcZpUSU6JRWK=
HTNGPkbXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NFPqeIdKSzVyPUCuNVM2PzRizszNNYXE[JRWW0GQR1XS
KP-N-RT-BM-1M4fqfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M2PKN2lEPTB;MD6xN|YyOiEQvF2=M1:zN3NCVkeHUh?=
NCI-H747MlLYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NFLiT2RKSzVyPUCuNVM4KM7:TR?=NYOzR2I3W0GQR1XS
NCCITNG\2OHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M2jHfWlEPTB;MD6xN|k{QSEQvF2=M{jwRXNCVkeHUh?=
MLMANV2y[VJkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NEHKcWRKSzVyPUCuNVQyPjdizszNMorEV2FPT0WU
LP-1NWTkeW1ET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NFz6O49KSzVyPUCuNVQzPCEQvF2=M3n0NHNCVkeHUh?=
CAL-148NU\uUppzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NUHXWFJkUUN3ME2wMlE1PDh|IN88US=>MoPqV2FPT0WU
GB-1M{jHRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MUDJR|UxRTBwMUS1NlYh|ryPMXfTRW5ITVJ?
COR-L88M4PtT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7Mlr0TWM2OD1yLkG0PFAzKM7:TR?=NXX5SnpIW0GQR1XS
NKM-1MnewS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NXiwRYJOUUN3ME2wMlE1QTB4IN88US=>M2\ieXNCVkeHUh?=
NB5MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NHPiNnBKSzVyPUCuNVU1PjJizszNMoHCV2FPT0WU
SH-4NVTTdY4xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NXTiN|NvUUN3ME2wMlE2PDZ4IN88US=>Mle3V2FPT0WU
EM-2MmLyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NIXYZ5FKSzVyPUCuNVU1PjdizszNMYDTRW5ITVJ?
TGBC24TKBMXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M4HIUWlEPTB;MD6xOVY2QSEQvF2=MX;TRW5ITVJ?
SCLC-21HM{\2OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7Mle3TWM2OD1yLkG1O|AzKM7:TR?=MYnTRW5ITVJ?
COLO-684M1HTW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MmX2TWM2OD1yLkG1O|IyKM7:TR?=NFflbmJUSU6JRWK=
KINGS-1Mme1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MorzTWM2OD1yLkG1PVEh|ryPMnfWV2FPT0WU
LS-123MnPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MVPJR|UxRTBwMUW5NVch|ryPM3rrT3NCVkeHUh?=
MC116MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MnnuTWM2OD1yLkG2NVU5KM7:TR?=Ml;mV2FPT0WU
SU-DHL-1NVfvOJpIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MWXJR|UxRTBwMU[yNFYh|ryPNI\KWmdUSU6JRWK=
NCI-H64M2nHPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NV3sUI1MUUN3ME2wMlE3OzR5IN88US=>M{POXXNCVkeHUh?=
HCE-TMnLDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NWjtZYNUUUN3ME2wMlE3PTB2IN88US=>NV\qT4E1W0GQR1XS
LOXIMVIMXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NVf2OVJFUUN3ME2wMlE3QTR7IN88US=>NXzhTVRMW0GQR1XS
ARH-77MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MW\JR|UxRTBwMUezO|kh|ryPNGOySGpUSU6JRWK=
DOHH-2MlLuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NFS1XItKSzVyPUCuNVc3PzJizszNNYDFfHZCW0GQR1XS
COLO-320-HSRM4jOOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M3vNTmlEPTB;MD6xPFE2PCEQvF2=MliyV2FPT0WU
NB6NGG3[2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MojaTWM2OD1yLkG4OVY3KM7:TR?=M3nxWHNCVkeHUh?=
L-428MkXjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NYK3NZFsUUN3ME2wMlE5QDF|IN88US=>NEjBS5BUSU6JRWK=
SNB75NWrxUZZDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NWXFbJhyUUN3ME2wMlE5QDJ2IN88US=>NEPXb3pUSU6JRWK=
NCI-H2141NHzoW29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=Mn63TWM2OD1yLkG5NFEyKM7:TR?=MUDTRW5ITVJ?
KARPAS-299NEjFcJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NI\LVGdKSzVyPUCuNVkzPDFizszNM16zS3NCVkeHUh?=
LC4-1NV3iepc5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NYHMN|Z{UUN3ME2wMlE6PTB{IN88US=>Mmi3V2FPT0WU
UACC-257NGDYNWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MljrTWM2OD1yLkG5PVQ4KM7:TR?=NYH1OGUzW0GQR1XS
EVSA-TM{PXc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7M4\1XGlEPTB;MD6yNFMyOiEQvF2=NU\vOo1lW0GQR1XS
JiyoyeP-2003NGC5N4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MXrJR|UxRTBwMkC0O|ch|ryPMYXTRW5ITVJ?
NCI-H720M4S5Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MW\JR|UxRTBwMkC1NlUh|ryPM1PLfHNCVkeHUh?=
GAKNYTjVJpQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MX\JR|UxRTBwMkGxPFkh|ryPNF7kUGZUSU6JRWK=
HHM3fiOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NWP4SZF3UUN3ME2wMlIyPDB3IN88US=>MmfTV2FPT0WU
NCI-H1436NWDw[o96T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NH7LXHVKSzVyPUCuNlE1PjRizszNNX3kZpg{W0GQR1XS
GR-STMmXUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NV\hRZRHUUN3ME2wMlIyPDh6IN88US=>M{fkUnNCVkeHUh?=
BC-1MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NVq2PYdJUUN3ME2wMlIzPjF4IN88US=>NEnYbY9USU6JRWK=
SIG-M5MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M4\kVGlEPTB;MD6yNlczPyEQvF2=M13nU3NCVkeHUh?=
NCI-H345MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MlnrTWM2OD1yLkKzPFA2KM7:TR?=NU\q[|JbW0GQR1XS
OPM-2M3rD[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7Mn;GTWM2OD1yLkKzPFgzKM7:TR?=MofqV2FPT0WU
LB1047-RCCNYDUOWxKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MnzTTWM2OD1yLkK0OFEh|ryPMoCwV2FPT0WU
CTB-1NVS3[4JvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MoLTTWM2OD1yLkK0OFk{KM7:TR?=M{W4dnNCVkeHUh?=
A3-KAWMkLNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NGrzcVdKSzVyPUCuNlU{PDFizszNNHnwTHBUSU6JRWK=
SF126MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M2j3dGlEPTB;MD6yOVM5OyEQvF2=M3[0[nNCVkeHUh?=
KARPAS-422NGrON5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NHTqRlhKSzVyPUCuNlU1OzVizszNM{PqU3NCVkeHUh?=
MEG-01M3PieGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M1rETmlEPTB;MD6yOVY4OyEQvF2=NUHzV2xEW0GQR1XS
NCI-H209NV;KNIh{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M4TV[WlEPTB;MD6yOlM4PCEQvF2=NVPhXYdrW0GQR1XS
SBC-1M{jRfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHG2bWJKSzVyPUCuNlY2KM7:TR?=M1rUXnNCVkeHUh?=
K5MnXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MUHJR|UxRTBwMk[5PFQh|ryPMVzTRW5ITVJ?
QIMR-WILMXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M1LEZmlEPTB;MD6yO|E2PSEQvF2=MmHuV2FPT0WU
NMC-G1NH\RZ29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NUnzTY41UUN3ME2wMlI5OjF5IN88US=>MmrCV2FPT0WU
IST-MES1NX\kVlBCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M2\sWmlEPTB;MD6yPVI{QCEQvF2=NH24U|FUSU6JRWK=
SW684M37QZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MWLJR|UxRTBwM{CwOFch|ryPNFK2fGZUSU6JRWK=
MV-4-11NFnjc3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NXXuZWlvUUN3ME2wMlMxPzh6IN88US=>NGrXfY1USU6JRWK=
LAN-6NGG5WmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NV;1bnlwUUN3ME2wMlMyPTd{IN88US=>MlriV2FPT0WU
RH-1M1TreGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M{fuW2lEPTB;MD6zNVg4OSEQvF2=MlX1V2FPT0WU
CP66-MELNHexUlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MnvrTWM2OD1yLkOxPFc5KM7:TR?=NETUbHVUSU6JRWK=
EC-GI-10NE\COXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MUjJR|UxRTBwM{K5PFch|ryPMlfjV2FPT0WU
GDM-1NGLDUGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NIn0eplKSzVyPUCuN|MxPTlizszNNYLCOXBLW0GQR1XS
IST-SL1M3KyTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHi5c3FKSzVyPUCuN|Q6OTRizszNMVTTRW5ITVJ?
KMOE-2M13yNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MkDwTWM2OD1yLkO1NVc2KM7:TR?=MYjTRW5ITVJ?
OS-RC-2NGXQeIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MkLWTWM2OD1yLkO1OVQ1KM7:TR?=MVrTRW5ITVJ?
HDLM-2MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M{LPZmlEPTB;MD6zO|gyPCEQvF2=M1LoOnNCVkeHUh?=
DSH1NUXXZoVVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NHjRN|dKSzVyPUCuN|g3OzFizszNNIXF[|RUSU6JRWK=
LS-1034M1L2bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MmfiTWM2OD1yLkO4PVU1KM7:TR?=Mmj6V2FPT0WU
LB373-MEL-DMmrtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MnS3TWM2OD1yLkO5NVk4KM7:TR?=MYLTRW5ITVJ?
GOTOMn7tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M1\U[WlEPTB;MD60NlEyQCEQvF2=MYjTRW5ITVJ?
MRK-nu-1M4Ljbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M3y2eWlEPTB;MD60NlIxPSEQvF2=NVfkWHhQW0GQR1XS
SK-MM-2MnHDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NGK0fWZKSzVyPUCuOFM1PDJizszNM4nV[XNCVkeHUh?=
BOKUM{DVTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MkfNTWM2OD1yLkSzO|kzKM7:TR?=MV3TRW5ITVJ?
NOMO-1MmD1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MUDJR|UxRTBwNEO4OkDPxE1?M4rpW3NCVkeHUh?=
J-RT3-T3-5NHPkZ|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MnLETWM2OD1yLkS0PFc5KM7:TR?=NFjyTo1USU6JRWK=
COLO-668MoT0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MlPOTWM2OD1yLkS1OlY5KM7:TR?=M{jHcHNCVkeHUh?=
SF539M3vre2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7M{e1cWlEPTB;MD60PFY5PCEQvF2=MUTTRW5ITVJ?
LB2518-MELNGDDS2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=Mlj5TWM2OD1yLkS5O|A{KM7:TR?=NVzxXZF[W0GQR1XS
TE-441-TMX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NUmwdZFRUUN3ME2wMlUxOTJizszNMoD5V2FPT0WU
MDA-MB-134-VINIrOPHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MUTJR|UxRTBwNUC5NlEh|ryPMnHkV2FPT0WU
SCC-3MkC5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M4nmdmlEPTB;MD61NVg4OyEQvF2=MkTYV2FPT0WU
KLENWDrR3VxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M33zRmlEPTB;MD61NlE4OSEQvF2=NITLdJhUSU6JRWK=
LB2241-RCCMVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?Mn\1TWM2OD1yLkWyOVI2KM7:TR?=NFzFRWlUSU6JRWK=
NCI-H1417NWG1d29UT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MUfJR|UxRTBwNUO0N|Uh|ryPNH\GUVlUSU6JRWK=
NCI-H2081MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M2XDfmlEPTB;MD61PFYyOSEQvF2=NGjMfm1USU6JRWK=
NCI-H1522NXXk[nFoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MVnJR|UxRTBwNUmzPFMh|ryPMWjTRW5ITVJ?
NCI-H889MkLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NIW5[YlKSzVyPUCuOVk5PDRizszNM1zxW3NCVkeHUh?=
CGTH-W-1Mm\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MUDJR|UxRTBwNkC3OVUh|ryPMXzTRW5ITVJ?
EB-3NIXwd4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MXvJR|UxRTBwNkGxOVMh|ryPMm\zV2FPT0WU
D-336MGNGX3TJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M2Dhc2lEPTB;MD62NVc6OSEQvF2=MUDTRW5ITVJ?
LC-1FMXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NXzQdIJmUUN3ME2wMlYzPTl{IN88US=>M{\0XXNCVkeHUh?=
MHH-NB-11NF;O[HpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M4jZemlEPTB;MD62N|gxOSEQvF2=NVSzN45SW0GQR1XS
SK-N-FINEDxd3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MXnJR|UxRTBwNkO5NUDPxE1?NGrCfZlUSU6JRWK=
NCI-H69NVPqeoZqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NXPGOpRNUUN3ME2wMlY1QDd7IN88US=>NGLYWJFUSU6JRWK=
SCHMWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NYfDZVJxUUN3ME2wMlY3ODJ|IN88US=>NYXoUWVnW0GQR1XS
LS-513NYmxR|FMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NFe3UlhKSzVyPUCuOlczPTlizszNNUXVXFZ3W0GQR1XS
CP67-MELMVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MnfFTWM2OD1yLk[3OVk3KM7:TR?=MYTTRW5ITVJ?
GCIYMVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MlnJTWM2OD1yLkewN|Y{KM7:TR?=NGrCSnFUSU6JRWK=
SHP-77M{LKO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7M4fVT2lEPTB;MD63NFc{PCEQvF2=M1q5[3NCVkeHUh?=
EHEBNEjBRmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M3Hve2lEPTB;MD63NVg2PCEQvF2=M3nucXNCVkeHUh?=
D-247MGMWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NF3BPFZKSzVyPUCuO|MzOzJizszNMoOxV2FPT0WU
RajiNXq1OnRNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MkTqTWM2OD1yLke0PVg6KM7:TR?=NYS5e2xsW0GQR1XS
MPP-89M4\TbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NYXOTW1DUUN3ME2wMlc2QTd6IN88US=>MlyyV2FPT0WU
EKVXM4TCR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MoCzTWM2OD1yLke5NFc{KM7:TR?=MoLvV2FPT0WU
HCC2218NWDUc2RtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MnPzTWM2OD1yLke5NVMyKM7:TR?=NIXrdXZUSU6JRWK=
KNS-81-FDMoH1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M1\PZWlEPTB;MD64NVAyOSEQvF2=MYfTRW5ITVJ?
HCC2157MljwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NIPJNnZKSzVyPUCuPFQ6PTFizszNMYrTRW5ITVJ?
BT-474M1Pv[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MV7JR|UxRTBwOEi4OVQh|ryPMonuV2FPT0WU
NCI-H748MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MnXpTWM2OD1yLkmxNkDPxE1?Mkn0V2FPT0WU
EW-24NHyzT3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MYTJR|UxRTBwOUKwNFIh|ryPM2fKZ3NCVkeHUh?=
D-263MGMkfiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NUn1dWlEUUN3ME2wMlk1PTF{IN88US=>NF7RNIVUSU6JRWK=
OMC-1NUTETYpIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NYnFVJNEUUN3ME2wMlk3Ozd|IN88US=>NWm2TIpiW0GQR1XS
NCI-H2196MmHHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NXjLd5Z{UUN3ME2wMlk4Pzd5IN88US=>MlT0V2FPT0WU
HAL-01MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MVnJR|UxRTBwOUm4O|Ih|ryPM2nZWHNCVkeHUh?=
NCI-H2107NHjHRpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NF;K[phKSzVyPUGuNFE5PTRizszNM{XRTXNCVkeHUh?=
LB996-RCCNVHtU2lKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M1jIV2lEPTB;MT6wPFA2OSEQvF2=MV3TRW5ITVJ?
NCI-H1395Ml7ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MULJR|UxRTFwMUC2O{DPxE1?NWr0eXg{W0GQR1XS
CHP-126NH;Y[5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M1ywTGlEPTB;MT6xOlE6QSEQvF2=M3HM[XNCVkeHUh?=
NB69M2LVT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7NF;OXFJKSzVyPUGuNlYyPDhizszNMkjsV2FPT0WU
CAS-1M1GzZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7NYq0c5BwUUN3ME2xMlM1OjNzIN88US=>NXfSWWx4W0GQR1XS
JVM-3M3HqPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MX;JR|UxRTFwNEe4OVUh|ryPMmG0V2FPT0WU
DMS-153MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MX7JR|UxRTFwNUe2O|Qh|ryPNEPqeoxUSU6JRWK=
SK-MEL-2MkTkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MUfJR|UxRTFwN{G3PFkh|ryPNVPzNItpW0GQR1XS
NCI-H1155NX\yVIxiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MnjMTWM2OD1zLkezOlc3KM7:TR?=NWLkeFN1W0GQR1XS
NCI-H1092NYfzdnN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M{\MRmlEPTB;MT63OlE5OiEQvF2=MlLRV2FPT0WU
LU-165M2jEbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MYHJR|UxRTFwOE[0O|Yh|ryPNVLNfJA1W0GQR1XS
HL-60NWWxbJpwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NWfuVYExUUN3ME2xMlk6QTB6IN88US=>M{XtUXNCVkeHUh?=
REHNHnzO2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MVzJR|UxRTJwMEC1PFIh|ryPM1jJe3NCVkeHUh?=
JVM-2M3jnbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M4P5PGlEPTB;Mj6wOlE{PSEQvF2=NYC3eZE6W0GQR1XS
MN-60MkTrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NEOxbYNKSzVyPUKuNVc{ODhizszNMmLLV2FPT0WU
SCC-15M{Tkemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NVzIWIZtUUN3ME2yMlIxOjdizszNM{XleHNCVkeHUh?=
SNU-C1NFXmc5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M2f3bWlEPTB;Mj6yNVM1OyEQvF2=MXvTRW5ITVJ?
SK-MEL-1NYWzTG9PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NF\Ec|RKSzVyPUKuN|IxPDdizszNNWC0XJNQW0GQR1XS
TGWNEjYUlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NUnCcYZKUUN3ME2yMlM2ODJ5IN88US=>NWLBOmJXW0GQR1XS
CA46NGjqeYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MVjJR|UxRTJwN{W5Olch|ryPM3TGVnNCVkeHUh?=
NCI-H23NVrwV|VIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NEWwdoRKSzVyPUKuPFA4QTFizszNNHXiNJVUSU6JRWK=
MMAC-SFM1nmXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MXXJR|UxRTJwOE[2Nlgh|ryPMnm5V2FPT0WU
NCI-H2171M1\GVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHXJNmdKSzVyPUOuNFM1PDhizszNMoOyV2FPT0WU
A704NWL2cYhIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NGj6dINKSzVyPUOuNVQyQTRizszNMUnTRW5ITVJ?
DBMV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M4HpRWlEPTB;Mz6yOVU4PiEQvF2=M2\2O3NCVkeHUh?=
NCI-H719MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M2T2bmlEPTB;Mz6zNFM5PCEQvF2=NHjNe41USU6JRWK=
MC-CARNITVb5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NE\t[5hKSzVyPUOuOVQ5OTdizszNNW\TSGVUW0GQR1XS
SIMAMkLiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M1XPW2lEPTB;Mz61PFQyPiEQvF2=Mn3HV2FPT0WU
SW872NEDxSlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NYO5eoE6UUN3ME2zMlc5OTBzIN88US=>MYjTRW5ITVJ?
HCC1187NGHEVotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NXj4N2VFUUN3ME2zMlc5OzF3IN88US=>MnTTV2FPT0WU
EW-13M4n6OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MkfhTWM2OD1|LkizOFE4KM7:TR?=MW\TRW5ITVJ?
NCI-SNU-1M4LkUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NE\5WYNKSzVyPUSuNlE6QTlizszNNVvYRZVCW0GQR1XS
NCI-H226NEfadXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NXvjOJR5UUN3ME20MlM{PjB4IN88US=>NVLzTo0xW0GQR1XS
RPMI-8866M2LvZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHy0WXlKSzVyPUSuN|YxOjlizszNM37ve3NCVkeHUh?=
OCI-AML2NUTQdIdST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MnLjTWM2OD12LkW1NlMh|ryPNITqe3VUSU6JRWK=
LU-65M3r5W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MmTmTWM2OD12LkmzNFM{KM7:TR?=NEjmd4ZUSU6JRWK=
SK-N-DZMXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MmPOTWM2OD13Lk[zOFY2KM7:TR?=Mnn2V2FPT0WU
NB14M2nNSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NVeyW4RsUUN3ME21Mlk4OTN3IN88US=>M163bHNCVkeHUh?=
COLO-829M2HsOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MWTJR|UxRTZwM{OzOVkh|ryPMl35V2FPT0WU
MHH-CALL-2NE\tTnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NYnmUpZIUUN3ME22MlUyPTB6IN88US=>NETvWoVUSU6JRWK=
NCI-H1770MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NH;4cVZKSzVyPU[uOlY6ODRizszNMWrTRW5ITVJ?
ECC4NHjmWJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NUnCTVhZUUN3ME22MlgzOTd7IN88US=>M{j4WHNCVkeHUh?=
A498MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NVr0U2RkUUN3ME22Mlg4OTl{IN88US=>M{DidHNCVkeHUh?=
DMS-79MoXuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NIfpXllKSzVyPUeuNFQ2OTJizszNNF;i[oZUSU6JRWK=
P30-OHKNF\RfZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M1rWUWlEPTB;Nz6yNVkxPiEQvF2=NInMT3JUSU6JRWK=
D-502MGMmDiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MYjJR|UxRTdwOUizOkDPxE1?M2GyO3NCVkeHUh?=
RCC10RGBMl3TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NVzVOG5YUUN3ME24MlA3OjhizszNNEPtVlVUSU6JRWK=
U-698-MNH\Ve5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MX\JR|UxRThwMkmyNlYh|ryPMYLTRW5ITVJ?
BB49-HNCM3TMeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NVXieoRTUUN3ME25MlQzOjZ3IN88US=>NX30VFlOW0GQR1XS
ES1M2m0N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MmS2TWM2OD17Lk[4PVU3KM7:TR?=MojFV2FPT0WU
MFM-223MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NIGwUGVKSzVyPUGwMlk6OjdizszNMkLVV2FPT0WU
C2BBe1M2fLVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NGHwc|VKSzVyPUGxMlM2OjNizszNNIXQZ|dUSU6JRWK=
EB2MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M2XzeWlEPTB;MUKuOlg5OSEQvF2=M{TCUXNCVkeHUh?=
C8166M{Tydmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7Mn\1TWM2OD1zMz64N|A{KM7:TR?=MnTmV2FPT0WU
ES3NXLS[nl7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MXzJR|UxRTF|Lkm5O|ch|ryPNWPWNYg3W0GQR1XS
NCI-H1650NHn4UHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MVHJR|UxRTF4LkO3OlUh|ryPMXTTRW5ITVJ?
Mo-TMoPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M37wcmlEPTB;MUeuOFAzQSEQvF2=NVfQT3M{W0GQR1XS
NB7MmfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M37QfWlEPTB;MUeuO|Q3PyEQvF2=MXPTRW5ITVJ?
ES7MkXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M4jVPGlEPTB;MUmuOVUxPyEQvF2=NWnNRYh5W0GQR1XS
ES5MnjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M3nFVWlEPTB;MkGuO|Y4OSEQvF2=NEnrXXpUSU6JRWK=
MSTO-211HNI\ROopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MYnJR|UxRTJzLki4OVgh|ryPMojuV2FPT0WU
U-87-MGM13ROWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M3\mVGlEPTB;MkWuNVk3PiEQvF2=M3K2bnNCVkeHUh?=
THP-1MnjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M{HWZmlEPTB;MkmuO|IxOSEQvF2=M1ra[3NCVkeHUh?=
NB10MlHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NV6xbGRtUUN3ME2zNE4zPjl2IN88US=>MV\TRW5ITVJ?
NH-12NV;0c2d{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NWDjWlc3UUN3ME2zN{4zODF5IN88US=>MnTZV2FPT0WU
COLO-824Mo\sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MlL3TWM2OD1|ND6xNFA3KM7:TR?=M2PkNnNCVkeHUh?=
SKM-1NETvRXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NHPFOmFKSzVyPUO3Mlg1OjJizszNMlX5V2FPT0WU
NCI-H1838NXXYXmRCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MUPJR|UxRTN6LkO3PVch|ryPNHznd|RUSU6JRWK=
RLMo[3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MnXYTWM2OD1|OT6yOFc4KM7:TR?=NELiUJJUSU6JRWK=
COLO-800MnLNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MkTjTWM2OD12MD6wO|c{KM7:TR?=M4HjUXNCVkeHUh?=
KG-1NEO4ZnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NHrJSXdKSzVyPUSwMlg6PTVizszNMnrqV2FPT0WU
TURM32zOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M1[4fWlEPTB;NEGuOlM6QSEQvF2=NFPMOZFUSU6JRWK=
LNCaP-Clone-FGCNET3d3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NGXyVGNKSzVyPUSxMlkxOTdizszNNX\sNWNwW0GQR1XS
NCI-H322MMnnVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MXjJR|UxRTR{LkW5PFQh|ryPNI\lfW1USU6JRWK=
LNCaP M{SweWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M33qSGlEPTB;OUGuN{BvVQ>?MmPMNlU2ODVzN{S=
C4-2MlTCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NU\NTYF[UUN3ME24JI5ONX;TW2pFOjV3MEWxO|Q>
JURKATMlvMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M{D3TVI1KGh?MUjJR|UxRTRzNj62OkBvVQ>?NVWxZnhPOjR3MUm5PVU>
MOLT-4NHPxSolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NUi2SXg3OjRiaB?=NWjrOpRpUUN3ME2yPFU{NjVibl2=NIrJeWEzPDVzOUm5OS=>
REHNYTZc|l2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MkHaNlQhcA>?MnLhTWM2OD1{OEeuOlghdk1?NHewTpEzPDVzOUm5OS=>
NALM-6NH3tbmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NGTKVoozPCCqMUXJR|UxRTN2M{OwJI5OMYCyOFUyQTl7NR?=
RS 4;11M{TOWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MYKyOEBpMljDTWM2OD13MUmuNFUhdk1?M2HJNlI1PTF7OUm1
697NXS4UGZuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M1TkZlI1KGh?Mn3JTWM2OD1{MUC3MlEhdk1?NIXacHgzPDVzOUm5OS=>
SIL-ALLNXjWOVZ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MWWyOEBpNITHWlRKSzVyPUG5PU44PyCwTR?=M4XwZlI1PTF7OUm1
CEMMWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MWqyOEBpMl30TWM2OD13Mj6xPEBvVQ>?MnuxNlQ2OTl7OUW=
P12Mn\xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M1OyOVI1KGh?MYXJR|UxRTN7LkK3JI5OM{PFe|I1PTF7OUm1
NALM-16MlfPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NFfseFYzPCCqMWHJR|UxRTl4LkK4JI5ONYe1cm1ROjR3MUm5PVU>
JURKATMXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M4P1XVQ5KGh?M3LoXWlEPTB;MUSuPFQhdk1?NX3mPXFPOjR3MUm5PVU>
MOLT-4NHzlOmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NEi1U|c1QCCqNULqTHJ{UUN3ME2yOk43KG6PMln0NlQ2OTl7OUW=
REHMnPvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MYG0PEBpMUPJR|UxRTR2LkexJI5ONYjLXG9kOjR3MUm5PVU>
NALM-6MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MV60PEBpM3\xNWlEPTB;MkOuNlYhdk1?MUeyOFUyQTl7NR?=
RS 4;11NXm0Z5RkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NYP1d3lzPDhiaB?=NX\Fb5RDUUN3ME2xOlQvOjhibl2=Ml7ENlQ2OTl7OUW=
697NFr1bFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MkTBOFghcA>?M3TJ[WlEPTB;M{C1MlMhdk1?MWSyOFUyQTl7NR?=
SIL-ALLM3LNbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NVvWXVBWPDhiaB?=NYO1NWtFUUN3ME23OE43OiCwTR?=NVvM[5F7OjR3MUm5PVU>
CEMM1jYdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7Mln1OFghcA>?MkeyTWM2OD1{ND61OUBvVQ>?NIrkR|QzPDVzOUm5OS=>
P12Mk\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M{nEXFQ5KGh?M4DITGlEPTB;MUiuOFYhdk1?NGLp[HozPDVzOUm5OS=>
NALM-16MofBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M4iwUFQ5KGh?NH\HfWtKSzVyPU[zMlI1KG6PMoDLNlQ2OTl7OUW=
JURKATMmXJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NInMS5I4OiCqNVPuOWhuUUN3ME2zMlY2KG6PM2\XRVI1PTF7OUm1
MOLT-4M3vqb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MXS3NkBpMX;JR|UxRTdwM{mgcm0>M4HjRlI1PTF7OUm1
REHMUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?Mn71O|IhcA>?MlznTWM2OD1zNj6wNUBvVQ>?MViyOFUyQTl7NR?=
NALM-6M3THSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M3rBVFczKGh?NYHvd4l5UUN3ME2xMlc5KG6PM2G1blI1PTF7OUm1
RS 4;11M4fPVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NV3jdnpHPzJiaB?=NYTJe|htUUN3ME20MlM5KG6PM4LoN|I1PTF7OUm1
697M4nlb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MYe3NkBpNH31RWpKSzVyPUe0MlI5KG6PMnrDNlQ2OTl7OUW=
SIL-ALLNX:x[ldFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MYm3NkBpMV;JR|UxRTdwMjDuUS=>MVSyOFUyQTl7NR?=
CEMM1vJVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MlLwO|IhcA>?MVXJR|UxRTVwNEKgcm0>NXnyNJdYOjR3MUm5PVU>
P12M33YN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7NULWWmhrPzJiaB?=MYnJR|UxRTZwM{egcm0>MYqyOFUyQTl7NR?=
NALM-16MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M1nsUlczKGh?MWPJR|UxRTd5LkezJI5OM2rN[lI1PTF7OUm1
Y79MlXaRZBweHSxc3nzJGF{e2G7NVfMdlNxOS13MDDuUS=>MVuxNk8zPCCqMkfBSG1UVw>?M1[wVIlv\HWlZXSgZZBweHSxc3nzJJdpcWOqIHPhckBj\SCnbnjhcoNm\CCkeTDIWC=>M1rsd|I1OzZ4Nk[1
WER-Rb-1NGnpWVlCeG:ydH;zbZMhSXO|YYm=MmDBNU02OCCwTR?=NWLHPXd4OTJxMkSgbC=>MoTXSG1UVw>?NGXoSmNqdmS3Y3XkJIFxd3C2b4Ppd{B4cGmlaDDjZY4h[mViZX7oZY5k\WRiYomgTHQ>M3z3d|I1OzZ4Nk[1
G7NXjsd3ZqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MmS3TWM2OD1|LkOgcm0>NWTKc5NoOjR{MES3N|M>
G166M1f2PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NWjZelB4UUN3ME23MlAhdk1?MWSyOFIxPDd|Mx?=
CB660NITrVI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NFLOepRKSzVyPU[uOEBvVQ>?MoPhNlQzODR5M{O=
CB1117Mn22S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MkPQTWM2OD1zNT62JI5OM3vCelI1OjB2N{Oz
KB-3-1NV7jT4ZQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MVHJR|UxRTFwNEigxtEhOC53MzDuUS=>MXeyN|k3OjR2NR?=
KB-8-5-11NXLGNpdlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NV3PcVhUUUN3ME21MlAyKMLzIEKuNFEhdk1?NEDLSIkzOzl4MkS0OS=>
KB-C-1NIHuTINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MWjJR|UxRTF4Lke2JOKyKDRwNUGgcm0>NXz3dJRWOjN7NkK0OFU>
KB-V-1MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MoTITWM2OD1{OEGuNlQhyrFiM{GuOVchdk1?NGPZ[JgzOzl4MkS0OS=>
OVCAR-8MkLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M4njSmlEPTB;Nz6xPUDDuSB|Lk[wJI5OMX2yN|k3OjR2NR?=
NCI-ADR-RESMV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M2XGcWlEPTB;MUK3N{4{OCEEsTCxPVEvOjBibl2=MlzpNlM6PjJ2NEW=
MCF7NXTsbGVDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M4fQ[WlEPTB;NT6xNUDDuSBzLkS3JI5OM3nqOlI{QTZ{NES1
MCF7-FLV1000NHe1ZZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NH73OGdKSzVyPUO5N{4yQCEEsTC3OE45OyCwTR?=M{PmZVI{QTZ{NES1
S1NGDxRlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MlPiTWM2OD1{ND6zNEDDuSB2Lke1JI5ONFK1flgzOzl4MkS0OS=>
S1-M1-80NG\iZnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NVXGe2t5UUN3ME2yOlEvODZiwsGgOFgvOjVibl2=NFm3U|YzOzl4MkS0OS=>
CORL-23/PMnPZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NF7EVmtKSzVyPUSuN|ghyrFiMT6yOUBvVQ>?MV:yN|k3OjR2NR?=
CORL-23/RM{PCdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MlLlTWM2OD13LkG2JOKyKDFwOEOgcm0>NV3SSXJyOjN7NkK0OFU>
NIH3T3MmLBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M4f6UGlEPTB;NECuOlAhyrFiND65O{BvVQ>?NF3ldoUzOzl4MkS0OS=>
NIH3T3-G185NF7zTItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MYjJR|UxRTJ2MUOuOFAhyrFiNEKzMlY3KG6PNUXRSGNkOjN7NkK0OFU>
pcDNA-HEK293MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MVrJR|UxRTJwN{WgxtEhOC54NzDuUS=>MViyN|k3OjR2NR?=
MDR19-HEK293M2LoO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7NXPnb3NFUUN3ME24OU42OCEEsTCxN{43PyCwTR?=MnTrNlM6PjJ2NEW=
R482-HEK293NXnmNVI1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M2XBR2lEPTB;MkWuPVchyrFiND65OUBvVQ>?NHnhU4EzOzl4MkS0OS=>
MRP1-HEK293NHPsS2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NF7aUmtKSzVyPUKuPVkhyrFiMT60PUBvVQ>?Mmq0NlM6PjJ2NEW=
SF188NFPtNoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MWixNE0yODBibl2=MXGyOE06PiCqM2LtfGROW09?M{nSNolvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZT2gZY5lKGOxbnPlcpRz[XSrb36tJIRmeGWmZX70JI1idm6nch?=M3vFWFI{QDh5NkS1
T98GM3jHTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MorrNVAuOTByIH7NMYOyOE06PiCqNYHTcYRoTE2VTx?=NWD4ZY5UcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZ49v[2WwdILheIlwdi1iZHXw[YRmdnRibXHucoVzMkfaNlM5QDd4NEW=
DU145MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NXLjVm1wTE2VTx?=NIPoV2hKSzVy4pk8OlAhdk1?M1rWbFI{QDh2NEK4
LNCaP MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MXTEUXNQNVnEOJB5UUN3MPMIwFc2KG6PNGPwTnEzOzh6NESyPC=>
PC3MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NVnHWmNRTE2VTx?=MVXJR|Ux6oj:MUe1JI5ONVHY[5pUOjN6OES0Nlg>
RT4MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NEXuU45KSzVyPUK3MlIyKG6PNVfkSFQ4OjN5OUK2N|k>
5637NWOyN3doT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MXLJR|UxRTR3LkS3JI5ONXnRPGJ5OjN5OUK2N|k>
T24NF;XWVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MXHJR|UxRTd7LkGyJI5OMVWyN|c6OjZ|OR?=
MCF7 MmO5SpVv[3Srb36gRZN{[Xl?MVixNFAhdk1?M{LWfVEhcA>?MWfEUXNQMU\y[YR2[2W|IITo[UBqdmS3Y4Tpc44hd2cEoFfy[YIyNMLiU3frNUzDqFC|Mj|CpHNmenCrbnGzMEBidmUEoGfpd5AzNIHpR5ozOzd5MEK0OC=>
KB-V1 NUC3[llCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M4HPdGlEPTB;Mz65NkDPxE1?MYWyN|U6OzF7Nh?=
Hec50coNVf3fINZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NYO5NYVlUUN3ME21JI5ONWLi[GM1OjNzNE[2PFc>
MDA-MB-468MVPBdI9xfG:|aYOgRZN{[Xl?MnXwNU82NzFyIH7NM{PvclI1NzR6L{eyJIg>M4fXN2ROW09?NYXVdmJ1cW6mdXPld{BieG:ydH;zbZMhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI>M{XBN|I{OTR2Mkm0
MCF10AMojXRZBweHSxc3nzJGF{e2G7NVXwNIhvOS93L{GwJI5OMWCyOE81QC95MjDoMWDEUXNQMVnpcoR2[2W|IHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?=MUiyN|E1PDJ7NB?=
Huh-7MmXtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NHzpc5oyNzFyL{GwNE8yODByIH7NMn6yOFghcA>?NGOzdXBl\WO{ZXHz[ZMhfGinIHPlcIwhfmmjYnnsbZR6KGSxc3Wt[IVx\W6mZX70cJk>MWSyNlc1PTV6Nx?=
Daoy Mk\tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M13YdFEvQDd3LUK0NEBvVQ>?NIXpUnQ4OiCqMl\pSG1UVw>?MUDJR|UxRTVibl2=NYrGSlVTOjJ|OUCyO|k>
ONS-76MnSwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NXzhNYNHOS56N{WtNlQxKG6PNF7TTpM4OiCqNX\XV5RqTE2VTx?=NYDsWmo{UUN3ME23MlUhdk1?M{HpOFIzOzlyMke5
SUM 149M3;hb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MoXJNE0yODBibl2=NVXQbHNnPzJiaB?=NVvNendScW6qaXLpeJMhfGinIHPlcIwhfmmjYnnsbZR6KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoFzNWX4UGNHOjJ|MEm5N|k>
MDA-MB-231NIKxcmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M33STlAuOTByIH7NNVviXnJwPzJiaB?=MUDpcohq[mm2czD0bIUh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wYYK=MYqyNlMxQTl|OR?=
HR5NXT3d5d{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MVmwMVExOCCwTR?=M33CVVczKGh?MnfQbY5pcWKrdIOgeIhmKGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnHyMkPmNlI{ODl7M{m=
BT-474-M1M32zdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NFTJNVgxNTFyMDDuUS=>NIn1eGw4OiCqNUfRdllmcW6qaXLpeJMhfGinIHPlcIwhfmmjYnnsbZR6KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoFzMmriNlI{ODl7M{m=
AU565MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MnLkNE0yODBibl2=MU[3NkBpM4L4cYlvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0fUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7hdi=>NWL1fZVtOjJ|MEm5N|k>
T47DNVHYUoNvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NHPwNmcxNTFyMDDuUS=>Mn36O|IhcA>?M{DZbIlvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0fUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7hdi=>NY\R[2liOjJ|MEm5N|k>
MCF7NXjrS2hqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MUSwMVExOCCwTR?=NHfQd284OiCqNFS3N|VqdmirYnn0d{B1cGViY3XscEB3cWGkaXzpeJkhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7uZZI>NH3JPJQzOjNyOUmzPS=>
HCT116p53(+/+)M4WwZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MlrCNVAuOTByMDDuUS=>NV3aR3JjOjRxNEigbC=>MYPpcohq[mm2czDj[YxtKHC{b3zp[oVz[XSrb36gZ49ueGyndHXsfUBifCB{NTDuUS=>MmfjNlIzPjJzN{G=
HCT116p53(-/-)NFHGOW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MmXqNVAuOTByMDDuUS=>MWqyOE81QCCqMYHpcohq[mm2czDj[YxtKHC{b3zp[oVz[XSrb36gZ49ueGyndHXsfUBifCB{NTDuUS=>MYiyNlI3OjF5MR?=
HeLa Mnn0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M1GwdVExNTFyMDDuUS=>NFjk[|c1QCCqNWT5Z4c5TE2VTx?=NFzsVG5KSzVyPUWyMlkhdk1?MmDwNlIxQDB{M{W=
HeLa MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MUixNE0yODBibl2=MY[3NkBpMoLZSG1UVw>?MkKzTWM2OD13MD61JI5OM1uzPVIzODhyMkO1
GBCNYj0VoJ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NFLvTlM4OiCqMYfJR|UxRTF2Lke4JI5OMoPuNlE6PjV5M{m=
GB-CL-1NYfO[21lT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NYD3SXhyPzJiaB?=NWXKS5VZUUN3ME21MlIzKG6PM1qzOVIyQTZ3N{O5
Mz-ChA-2M2[0[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NFLrcmw4OiCqMUHJR|UxRTNwM{Ggcm0>NIT5ZnMzOTl4NUezPS=>
TGBC1-TKBM1X1Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MmLiNVQ1KGh?NFK0R5hKSzVyPU[uO|Ihdk1?MWOyNVk3PTd|OR?=
WittierNWrsdINyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NHXPZmg4OiCqM3vzSmlEPTB;Mz6yOkBvVQ>?MmDRNlE6PjV5M{m=
H-1M2LkVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M{XTNlczKGh?MUTJR|UxRTlwNUSgcm0>NWnkOnNLOjF7NkW3N|k>
HuH-28MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NIDpRmc4OiCqNUn1flYxUUN3ME2xNU4yQSCwTR?=MXeyNVk3PTd|OR?=
NECNFjyVmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NFPNO4k4OiCqMYLJR|UxRTlwOUigcm0>NYfW[FY5OjF7NkW3N|k>
REBM3qxWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHm0N4k4OiCqNWT0XJQ6UUN3ME2xN{42OSCwTR?=NGnR[ZUzOTl4NUezPS=>
CC-LP-1NHu2OmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NH:xUIs4OiCqM2fNTmlEPTB;NT64OEBvVQ>?NYDr[2ZDOjF7NkW3N|k>
CC-SW-1MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?Mk[3O|IhcA>?M4PJN2lEPTB;OT61OUBvVQ>?MYCyNVk3PTd|OR?=
EGI-1MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MWW3NkBpMVfJR|UxRTFyLkeyJI5OM3fUTFIyQTZ3N{O5
Sk-ChA-1MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M1rr[VczKGh?NV;ROpFnUUN3ME2xO{4zKG6PMWSyNVk3PTd|OR?=
TFK-1NF30O5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MkTkO|IhcA>?MVrJR|UxRTF|LkS1JI5OMVqyNVk3PTd|OR?=
786-OM{HvNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MmT6OU05OCCwTR?=NIXq[|E6PiCqNX63e48{UEOuM1v0WJN2eHC{ZYPz[ZMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZIhf2m2aDDJR|UxyqC4YXz1[ZMhd2ZiMjD0c{AzOCCwTR?=MWOyNVY1OjN5NB?=
A498MkD4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M3nQd|UuQDBibl2=Mmm4PVYhcA>?NFrmcnRJS2x?M2iybZN2eHC{ZYPz[ZMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZIhf2m2aDDJR|UxyqC4YXz1[ZMhd2ZiMjD0c{AzOCCwTR?=NEOzUGIzOTZ2MkO3OC=>
ACHNM1jmbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M2PsSlUuQDBibl2=M1zjZ|k3KGh?NHXJRmpJS2x?NIDSZol{fXCycnXzd4V{KHSqZTDwdo9tcW[ncnH0bY9vKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVzKHerdHigTWM2OMLidnHseYV{KG:oIEKgeI8hOjBibl2=NVnIWIp4OjF4NEKzO|Q>
Caki-1NF;FVpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MlL5OU05OCCwTR?=M{TRNlk3KGh?MlHJTGNtM4\1O5N2eHC{ZYPz[ZMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZIhf2m2aDDJR|UxyqC4YXz1[ZMhd2ZiMjD0c{AzOCCwTR?=NYrXb|lPOjF4NEKzO|Q>
SN12CNF3p[otIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MmjCOU05OCCwTR?=M1LlSFk3KGh?NUTNSWJRUEOuNITGVmN{fXCycnXzd4V{KHSqZTDwdo9tcW[ncnH0bY9vKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVzKHerdHigTWM2OMLidnHseYV{KG:oIEKgeI8hOjBibl2=M{\jbFIyPjR{M{e0
HMC-1.1M2Swbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHK5S|MyNTFyMECgcm0>MnPiO|IhcA>?MYXpcohq[mm2czD0bIUh[2WubDDwdo9tcW[ncnH0bY9vKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoU>MX6yNVI1OjF6OR?=
HMC-1.2NVXOW2lrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MYexMVExODBibl2=MWS3NkBpMmTYbY5pcWKrdIOgeIhmKGOnbHygdJJwdGmoZYLheIlwdiCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5mNWPHTFJQOjF{NEKxPFk>
C2NX3RPVhET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MmjFNU0yODByIH7NNUjrXXRGPzJiaB?=M3vve4lvcGmkaYTzJJRp\SClZXzsJJBzd2yrZnXyZZRqd25iaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\Q>?MXeyNVI1OjF6OR?=
CHP-212MkPKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M1q1d|EuOTJ3IH7NMUGyOE0yOjBiaB?=M3TzbIlvcGmkaYTzJJRp\SClZXzsJJBzd2yrZnXyZZRqd25iaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnW=MYGyNVE3QTJ2Mh?=
IMR-32M3\XPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NVrKTVl1OS1zMkWgcm0>NHq5dFUzPC1zMkCgbC=>NVSzWlNjcW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbjDpckBjd3SqIITpcYUuKGGwZDDkc5NmNWSncHXu[IVvfCCvYX7u[S=>MXKyNVE3QTJ2Mh?=
KellyM2jvWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MWKxMVEzPSCwTR?=M1HiVVI1NTF{MDDoNYLtSohlcW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbjDpckBjd3SqIITpcYUuKGGwZDDkc5NmNWSncHXu[IVvfCCvYX7u[S=>MV:yNVE3QTJ2Mh?=
SH-EPNF[xcXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NYfxPIRSOS1zMkWgcm0>NG\WfWQzPC1zMkCgbC=>NV7nNZY1cW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbjDpckBjd3SqIITpcYUuKGGwZDDkc5NmNWSncHXu[IVvfCCvYX7u[S=>NHrvZ3MzOTF4OUK0Ni=>
SH-SY5YNV\RZ2dnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M4fKRVEuOTJ3IH7NMlPINlQuOTJyIHi=M{jDT4lvcGmkaYTzJJRp\SClZXzsJJBzd2yrZnXyZZRqd25iaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnW=MYGyNVE3QTJ2Mh?=
SK-N-ASMknVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NGDyW4QyNTF{NTDuUS=>MVSyOE0yOjBiaB?=NY\lRmxrcW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbjDpckBjd3SqIITpcYUuKGGwZDDkc5NmNWSncHXu[IVvfCCvYX7u[S=>NV\0eIh7OjFzNkmyOFI>
SK-N-BEM1q5Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7NW\KV4h6OS1zMkWgcm0>MYmyOE0yOjBiaB?=M1fnOIlvcGmkaYTzJJRp\SClZXzsJJBzd2yrZnXyZZRqd25iaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnW=M3;aPVIyOTZ7MkSy
KG1NX;aUnNUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MXSxM|ExNzFyMDDuUS=>M3TiZlczKGh?Ml36SG1UVw>?MWHpcohq[mm2czD0bIUh[2WubDDwdo9tcW[ncnH0bY9vKGmwIHLveIghfGmvZT2gZY5lKGSxc3Wt[IVx\W6mZX70JI1idm6nMli1NVk1PTh|NUi=
U937M2jrdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MWSxM|ExNzFyMDDuUS=>MV:3NkBpNF34WoxFVVORNVnudplJcW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbjDpckBjd3SqIITpcYUuKGGwZDDkc5NmNWSncHXu[IVvfCCvYX7u[S=>MknINVk1PTh|NUi=
CAL62MnrzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NIr3[40xNjFvMUCwJI5OMUS3NkBpM{jISGROW09?M2PPbmVEPTB;MT60JI5OM{X4TFE6OjJ|NUWz
OCUT-1NHfvZXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M{nmfVAvOS1zMECgcm0>NGG4W5c4OiCqM1fHXWROW09?MU\FR|UxRTJwMzDuUS=>MUSxPVIzOzV3Mx?=
8505CMkDkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MWqwMlEuOTByIH7NMl;RO|IhcA>?NWPvO5pRTE2VTx?=NVSyPYg2TUN3ME2zMlIhdk1?NXTaOZlmOTl{MkO1OVM>
ACT-1M4D6O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MV6wMlEuOTByIH7NNGnaUFg4OiCqMlrLSG1UVw>?NU\Bd|RbTUN3ME21MlQhdk1?MWOxPVIzOzV3Mx?=
SW1736NGL1NFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NYD1UpRTOC5zLUGwNEBvVQ>?MVm3NkBpMULEUXNQM4\vT2VEPTB;NT62JI5OMn[5NVkzOjN3NUO=

... Click to View More Cell Line Experimental Data

In vivo BI 2536 given i.v. once or twice per week is highly efficacious in diverse xenograft models with acceptable tolerability by inhibiting cell proliferation through a mitotic arrest, and subsequently induction of tumor-cell death. Administration of BI 2536 at 50 mg/kg once or twice per week significantly inhibits growth of HCT 116 xenografts with T/C of 15% and 0.3%, respectively. BI 2536 treatment twice-weekly also leads to excellent tumor-growth in BxPC-3 and A549 models with T/C of 5% and 14%, respectively. [1]
Features The first potent and selective Plk1 inhibitor that induces all hallmarks of Plk1 inhibition.

Protocol(Only for Reference)

Kinase Assay: [1]

Plk1 in vitro kinase assay Recombinant human Plk1 (residues 1-603) is expressed as N-terminal, GST-tagged fusion protein with a baculoviral expression system and purified by affinity chromatography with Glutathione-agarose. Enzyme activity assays for Plk1 are performed in the presence of serially diluted BI 2536 with 20 ng of recombinant kinase and 10 μg casein from bovine milk as the substrate. Kinase reactions are performed in a final volume of 60 μL for 45 minutes at 30 °C (15 mM MgCl2, 25 mM MOPS [pH 7.0], 1 mM DTT, 1% DMSO, 7.5 mM ATP, 0.3 μCi γ-33P-ATP). Reactions are terminated by the addition of 125 μL of ice-cold 5% TCA. After transfer of the precipitates to Multi-Screen mixed ester cellulose filter plates, plates are washed with 1% TCA and quantified radiometrically. Dose-response curve is used for calculating IC50 value.

Cell Assay: [1]

Cell lines HeLa, A43, SKOV-3, HT-29, K562, A549, Saos-2, MCF7, HCT116, COLO 205, Hep G2, Raji and PC-3 cells, etc.
Concentrations Dissolved in DMSO, final concentrations ~1 μM
Incubation Time 24 and 72 hours
Method Cells are exposed to various concentrations of BI 2536 for 24, and 72 hours. Cell growth is assessed by the measurement of Alamar Blue dye conversion in a fluorescence spectrophotometer. For determining the DNA content of the cultures, cell suspensions are fixed in 80% ethanol, treated for 5 minutes with 0.25% Triton X-100 in PBS, and incubated with 0.1% RNase and 10 μg/mL propidium iodide (PI) in PBS for 20 minutes at RT. Cell-cycle profiles are determined by flow cytometric analysis.

Animal Study: [1]

Animal Models Female BomTac:NMRI-Foxn1nu mice injected subcutaneously with HCT 116, NCI-H460, or A549 cells
Formulation Formulated in hydrochloric acid (0.1 N), and diluted with 0.9% NaCl
Dosages ~50 mg/kg
Administration Injection i.v. once or twice per week

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)0.020.151.80.40.0810
Body Surface Area (m2)0.0070.0250.150.050.020.5
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

References

[1] Steegmaier M, et al. Curr Biol, 2007, 17(4), 316-322.

[2] Nappi TC, et al. Cancer Res, 2009, 69(5), 1916-1923.

Clinical Trial Information( data from http://clinicaltrials.gov, updated on 2016-07-30)

NCT Number Recruitment Conditions Sponsor
/Collaborators
Start Date Phases
NCT00526149 Completed Breast Cancer|Endometrial Cancer|Head and Neck Cancer|Melanoma (Skin)|Ovarian Cancer|Sarcoma European Organisation for Research and Treatment of Cance  ...more European Organisation for Research and Treatment of Cancer - EORTC July 2007 Phase 2
NCT02215044 Terminated Pancreatic Neoplasms Boehringer Ingelheim June 2007 Phase 1
NCT00412880 Completed Carcinoma, Small Cell Boehringer Ingelheim January 2007 Phase 2
NCT02211833 Completed Carcinoma, Non-Small-Cell Lung Boehringer Ingelheim October 2006 Phase 1
NCT00701766 Completed Leukemia, Myeloid, Acute Boehringer Ingelheim October 2006 Phase 2

view more

Chemical Information

Download BI 2536 SDF
Molecular Weight (MW) 521.66
Formula

C28H39N7O3

CAS No. 755038-02-9
Storage 3 years -20℃powder
2 years -80℃in solvent
Synonyms N/A
Solubility (25°C) * In vitro Ethanol 100 mg/mL (191.69 mM)
DMSO 21 mg/mL (40.25 mM)
Water <1 mg/mL
In vivo 30% PEG 400+0.5% Tween 80+5% Propylene glycol 15mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name (R)-4-(8-cyclopentyl-7-ethyl-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-2-ylamino)-3-methoxy-N-(1-methylpiperidin-4-yl)benzamide

Frequently Asked Questions

  • Question 1
    We need to use BI 2536 in preclinical studies using mice, how to formulate the inhibitor for this in vivo study?

    Answer: For in vivo study, DMSO should not be more than 5% because the toxicity. You can formulated BI2536 in hydrochloric acid (0.1 N), and diluted with 0.9% NaCl for animal study according to the reference :( Supplemental Data) http://linkinghub.elsevier.com/retrieve/pii/S0960-9822(06)02671-6

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

* Indicates a Required Field

Related PLK Products

  • SBE 13 HCl

    SBE 13 HCl is a potent and selective PLK1 inhibitor with IC50 of 200 pM, >4000-fold selectivity over Aurora A kinase, Plk2 and Plk3.

  • Ro-3306

    RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

  • CCG-1423

    CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.

  • Rigosertib (ON-01910)

    Rigosertib (ON-01910) is a non-ATP-competitive inhibitor of PLK1 with IC50 of 9 nM in a cell-free assay. It shows 30-fold greater selectivity against Plk2 and no activity to Plk3. Phase 3.

  • HMN-214

    HMN-214 is a prodrug of HMN-176, which alters the cellular spatial orientation of Plk1.

  • GSK461364

    GSK461364 inhibits purified Plk1 with Ki of 2.2 nM in a cell-free assay. It is more than 1000-fold selective against Plk2/3. Phase 1.

    Features:Demonstrates >390-fold selectivity for Plk1 relative to Plk2 and Plk3.

  • Volasertib (BI 6727)

    Volasertib (BI 6727) is a highly potent Plk1 inhibitor with IC50 of 0.87 nM in a cell-free assay. It shows 6- and 65-fold greater selectivity against Plk2 and Plk3. Phase 3.

    Features:A high volume of distribution, indicating good tissue penetration, and a long terminal half-life.

  • MLN0905

    MLN0905 is a potent inhibitor of PLK1 with IC50 of 2 nM.

  • Ro3280

    RO3280 is a potent, highly selective inhibitor of Polo-like kinase 1 (PLK1) with IC50 of 3 nM.

    Features:A selective PLK1 inhibitor. Demonstrates greater potency than BI2536. Potential use in nasopharyngeal carcinomas.

  • NMS-P937 (NMS1286937)

    NMS-P937 (NMS1286937) is an orally available, selective Polo-like Kinase 1 (PLK1) inhibitor with IC50 of 2 nM, 5000-fold selectivity over PLK2/PLK3. Phase 1.

Recently Viewed Items

Tags: buy BI 2536 | BI 2536 supplier | purchase BI 2536 | BI 2536 cost | BI 2536 manufacturer | order BI 2536 | BI 2536 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Contact Us