WM-3835

Catalog No.S9805

For research use only.

WM-3835 is a novel and high-specific small molecule Lysine Acetyltransferase 7 (KAT7, MYST2, HBO1) inhibitor, able to potently suppressed OS cell proliferation and migration, and leads to apoptosis activation.

WM-3835 Chemical Structure

CAS No. 2229025-70-9

Purity & Quality Control

Choose Selective Histone Acetyltransferase Inhibitors

Biological Activity

Description WM-3835 is a novel and high-specific small molecule Lysine Acetyltransferase 7 (KAT7, MYST2, HBO1) inhibitor, able to potently suppressed OS cell proliferation and migration, and leads to apoptosis activation.
Targets
KAT7 [1]
In vitro

WM-3835 activates apoptosis while inhibites OS cell proliferation, migration and invasion. The activity of WM-3835 is nullified in HBO1-KO OS cells. Also, the HBO1 inhibitor is non-cytotoxic in HBO1-low human osteoblasts, which indicates that the anti-OS cell efficacy of WM-3835 is primarily through HBO1 inhibition.[1]

In vivo

In vivo, intraperitoneal injection of a single dose of WM-3835 potently inhibites OS xenograft growth in SCID mice.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Primary human OS cells (pOS-1 cells, pOS-2 cells)
  • Concentrations: 1 μM, 5 μM, 10 μM, 25 μM
  • Incubation Time: 24 h, 48 h, 72 h, 96 h
  • Method:

    The pOS-1 cells are treated with WM-3835 or vehicle control (“Veh”, 0.5% DMSO), cells with treatments are seeded into 96-well plates at 3×103 cells per well. After 96 h incubation, in each well 10 μL CCK-8 reagent is added for 3 h. Later, CCK-8's OD value is recorded at 450 nm. Cell death is tested by Trypan blue staining using an automatic cell counter.

Animal Research:

[1]

  • Animal Models: SCID mice
  • Dosages: 10 mg/kg
  • Administration: IP

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 400.42
Formula

C20H17FN2O4S

CAS No. 2229025-70-9
Storage 3 years -20°C powder
2 years -80°C in solvent

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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