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Tween 80 Hydrotropic Agents chemical

Name: Tween 80
Brand: Selleck Chemicals
SKU: S6702
Availability: InStock
Rating: 5 (59 reviews)

Cat.No.S6702

Tween 80 is a polysorbate surfactant used for selective protein extraction and the isolation of nuclei from mammalian cell lines. This compound has been used in drug vehicle solutions for specific injections in mice and rats. It has been used for the determination of esterase activity in bacterial cells.

Chemical Structure

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Quality Control

Batch: Purity: 99%

Related Targets	PROTAC Others Dyes Antineoplastic and Immunosuppressive Antibiotics ADC Cytotoxin Selection Antibiotics for Transfected Cell Antibiotics for Mammalian Cell Culture ADC Linker Molecular Glues Antibiotics for Plant Cell Culture
Other Hydrotropic Agents Inhibitors	SBE-β-CD PEG300 CMC-Na PEG400 Corn Oil Methyl-β-cyclodextrin (MβCD) 1-Methoxy PMS Pluronic F-68 NOTA Castor oil

Solubility

In vitro
Batch:

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight		Formula	Storage (From the date of receipt)	2 years room temperature liquid
CAS No.	9005-65-6	--	Storage (From the date of receipt)	2 years room temperature liquid
Synonyms	Polysorbate 80		SMILES	CCCCCCCCC=CCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

References	[1] https://pubmed.ncbi.nlm.nih.gov/18804635/ [2] https://pubmed.ncbi.nlm.nih.gov/2231677/

Applications

Methods	Biomarkers	PMID
Western blot	p-TAK1 / TAK1 / p-JNK / JNK / p-p38 / p38 / n-p65 / c-p65 /p20 / β-actin p-TAK1 / TAK1 / p-JNK / JNK / p-p38 / p38 / n-p65 / c-p65 /p-Smad3 / Smad3 / β-actin	28698801
Growth inhibition assay	Pellet size	30263840
IHC	H&E / Col I / Col III	28698801
Microencapsulation	microcapsule morphology	30105055
Immunofluorescence	worms	33325720

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2013-03-14)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01807845	UNKNOWN	Vitamin D Deficiency	Rambam Health Care Campus	2013-01

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