CAS No. 9005-65-6
| CAS Number | 9005-65-6 | |
|---|---|---|
| Molecular Formula | ||
| SMILES | CCCCCCCCC=CCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO |
Tween 80 (Polysorbate 80) is a non-ionic surfactant and detergent that facilitates selective protein extraction and cell membrane solubilization. It disrupts lipid bilayer interactions without disrupting non-covalent protein complexes, allowing the isolation of intact nuclei and organelle fractions from mammalian cells. Additionally, Tween 80 serves as a substrate for evaluating bacterial esterase activity and acts as a stabilizing excipient that prevents protein aggregation.
| Solvent | Solubility & Note |
|---|
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer significantly longer shelf life compared to stock solutions, ensuring maximum stability for every use.
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