Home Ubiquitin E3 Ligase inhibitor NSC232003

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NSC232003 E3 Ligase inhibitor

Cat.No.E0351

NSC232003 is a highly potent and cell-permeable UHRF1 inhibitor that binds to the 5mC binding pocket of the SRA domain of UHRF1. This compound modulates DNA methylation in a cellular context.
NSC232003 E3 Ligase inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 169.14

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: E035101 Water]5 mg/mL]false]DMSO]Insoluble]false]Ethanol]Insoluble]false Purity: 99.89%
99.89

Chemical Information, Storage & Stability

Molecular Weight 169.14 Formula

C6H7N3O3

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 1905453-18-0 -- Storage of Stock Solutions

Synonyms N/A Smiles CC(NO)=C1C=NC(=O)NC1=O

Solubility

In vitro
Batch:

Water : 5 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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%DMSO %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC50/Ki
UHRF1 [1]
In vitro

NSC232003 is an effective DNA methylation inhibitor, and this compound can significantly decrease the DNMT1/UHRF1 interaction in U251 glioma cells.[2]
References

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