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Apcin E3 Ligase inhibitor

Name: Apcin
Brand: Selleck Chemicals
SKU: S9605
Availability: InStock
Rating: 5 (7 reviews)

Cat.No.S9605

Apcin (APC inhibitor) is an inhibitor of the E3 ligase activity of the mitotic anaphase-promoting complex/cyclosome (APC/C) that binds to Cdc20 and competitively inhibits the ubiquitylation of D-box-containing substrates.

Chemical Structure

Molecular Weight: 438.65

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Quality Control

Batch: S960501 DMSO]88 mg/mL]false]Ethanol]10 mg/mL]false]Water]Insoluble]false Purity: 99.49%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS
Datasheet

99.49

Related Targets	Proteasome E1 Activating DUB p97 SUMO E2 conjugating
Other E3 Ligase Inhibitors	Skp2 inhibitor C1 (SKPin C1) Iberdomide (CC-220) PRT4165 Avadomide (CC-122) SZL P1-41 Mezigdomide (CC-92480) Homo-PROTAC cereblon degrader 1 MRT-2359 SMER3 SMIP004

Solubility

In vitro
Batch:

DMSO : 88 mg/mL (200.61 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 10 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.000mg/ml (9.12mM)	Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	6.600mg/ml (15.05mM)	Taking the 1 mL working solution as an example, add 50 μL of 132 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	438.65	Formula	C₁₃H₁₄Cl₃N₇O₄	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	300815-04-7	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC1=NC=C(N1CCOC(=O)NC(C(Cl)(Cl)Cl)NC2=NC=CC=N2)[N+](=O)[O-]

Mechanism of Action

Targets/IC₅₀/Ki	APC/C CDC20
In vitro	Apcin is a novel cell‑permeable molecule that blocks the interaction between APC/C and Cdc20. This compound is demonstrated to inhibit OS cell growth and induce significant apoptosis. The invasion and mobile abilities of OS cells are also markedly suppressed by this chemical treatment. Furthermore, Bim and p21 are upregulated in OS cells following this treatment. The inhibition of CDC20 with its inhibitor Apcin reduces the adhesion rate and proliferation ability to RL95-2 and HEC-1A cells, respectively.
In vivo	CDC20 inhibitor Apcin inhibits embryo implantation in vivo.
References	[1] https://pubmed.ncbi.nlm.nih.gov/25156254/ [2] https://pubmed.ncbi.nlm.nih.gov/29901174/ [3] https://pubmed.ncbi.nlm.nih.gov/32458533/

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