Muramyl dipeptide

For research use only.

Catalog No.S9709 Synonyms: MDP

1 publication

Muramyl dipeptide Chemical Structure

CAS No. 53678-77-6

Muramyl dipeptide (MDP) is a shared structural unit of peptidoglycans. Muramyl dipeptide is an inducer of bone formation through the induction of Runx2.

Selleck's Muramyl dipeptide has been cited by 1 publication

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Biological Activity

Description Muramyl dipeptide (MDP) is a shared structural unit of peptidoglycans. Muramyl dipeptide is an inducer of bone formation through the induction of Runx2.
Targets
Runx2 [1]
()
In vitro

MDP directly augments osteoblast differentiation and bone-forming gene expression by Runx2 activation. Despite no direct effect, MDP indirectly attenuates osteoclast differentiation through down-regulation of the RANKL/OPG ratio. MDP increases the expression of the MDP receptor, Nod2, and MDP-induced bone formation and osteoblast activation does not occur during Nod2-deficiency.[1]

In vivo

Increased bone and mineral density by enhanced bone formation are observed in mice administered with Muramyl dipeptide (MDP). Remarkably, pre-treatment or post-treatment with MDP alleviates bone loss in RANKL-induced osteoporosis mouse models.[1]

Protocol

Cell Research:

[1]

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  • Cell lines: MC3T3-E1 cells, BMSCs, or primary osteoblast precursors from mouse calvaria
  • Concentrations: 0.1 μg/ml, 1 μg/ml, 10 μg/ml
  • Incubation Time: 2 days
  • Method:

    MC3T3-E1 cells, BMSCs, or primary osteoblast precursors from mouse calvaria are plated onto 48-well plates at 2×104 cells/400 μl/well and incubated with osteoblast induction medium in the absence or presence of MDP. Half of the medium is replaced with fresh osteoblast induction medium every 2 days. At day 6 and 12, the cells are stained with ALP for osteoblast differentiation and with alizarin red S for bone mineralization, respectively.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Five-week-old C57BL/6 mice, one-day-old neonatal mice, B6.129S1-Nod2tm1Flv/J mice
  • Dosages: 1.25 mg/kg
  • Administration: IP
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 98 mg/mL (198.99 mM)
Water 98 mg/mL (198.99 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 492.48
Formula

C19H32N4O11

CAS No. 53678-77-6
Storage powder
in solvent
Synonyms MDP

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04888767 Not yet recruiting Other: Interval Training Hight Intensity Program Cystic Fibrosis Fondation Ildys|Lille Catholic University September 1 2021 Not Applicable
NCT04341753 Recruiting Other: pulmonary rehabilitation Chronic Obstructive Pulmonary Disease University Hospital Brest September 14 2020 Not Applicable
NCT04087590 Recruiting Drug: Formoterol-glycopyrronium co suspension (PT003) Copd University Hospital Lille|AstraZeneca January 14 2020 Phase 2
NCT04370990 Recruiting Device: O2matic COPD|Hypoxia|Closed-Loop Communication|Dyspnea|Anxiety|Depression Copenhagen University Hospital Hvidovre September 1 2019 Not Applicable
NCT04375917 Recruiting Device: O2matic COPD|Dyspnea|Anxiety|Depressive Symptoms|Hypoxia|Closed-Loop Communication Copenhagen University Hospital Hvidovre September 1 2019 Not Applicable

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID