Home Cell Cycle RUNX activator Muramyl dipeptide (MDP) For research use only.

Muramyl dipeptide (MDP)

Muramyl dipeptide (MDP) is a shared structural unit of peptidoglycans. Muramyl dipeptide is an inducer of bone formation through the induction of Runx2.

Muramyl dipeptide (MDP) Chemical Structure

Muramyl dipeptide (MDP) Chemical Structure

CAS: 53678-77-6

Selleck's Muramyl dipeptide (MDP) has been cited by 2 publications

Purity & Quality Control

Batch: S970901 DMSO] 98 mg/mL] false] Water] 98 mg/mL] false] Ethanol] Insoluble] false Purity: >97%
97

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Biological Activity

Description Muramyl dipeptide (MDP) is a shared structural unit of peptidoglycans. Muramyl dipeptide is an inducer of bone formation through the induction of Runx2.
Targets
Runx2 [1]
In vitro
In vitro

MDP directly augments osteoblast differentiation and bone-forming gene expression by Runx2 activation. Despite no direct effect, MDP indirectly attenuates osteoclast differentiation through down-regulation of the RANKL/OPG ratio. MDP increases the expression of the MDP receptor, Nod2, and MDP-induced bone formation and osteoblast activation does not occur during Nod2-deficiency.[1]
Cell Research Cell lines MC3T3-E1 cells, BMSCs, or primary osteoblast precursors from mouse calvaria
Concentrations 0.1 μg/ml, 1 μg/ml, 10 μg/ml
Incubation Time 2 days
Method

MC3T3-E1 cells, BMSCs, or primary osteoblast precursors from mouse calvaria are plated onto 48-well plates at 2×104 cells/400 μl/well and incubated with osteoblast induction medium in the absence or presence of MDP. Half of the medium is replaced with fresh osteoblast induction medium every 2 days. At day 6 and 12, the cells are stained with ALP for osteoblast differentiation and with alizarin red S for bone mineralization, respectively.
In Vivo
In vivo

Increased bone and mineral density by enhanced bone formation are observed in mice administered with Muramyl dipeptide (MDP). Remarkably, pre-treatment or post-treatment with MDP alleviates bone loss in RANKL-induced osteoporosis mouse models.[1]
Animal Research Animal Models Five-week-old C57BL/6 mice, one-day-old neonatal mice, B6.129S1-Nod2tm1Flv/J mice
Dosages 1.25 mg/kg
Administration IP
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06079567 Recruiting
Facioscapulohumeral Muscular Dystrophy Type 2
Centre Hospitalier Universitaire de Nice
 October 3 2023 Not Applicable
NCT06045338 Not yet recruiting
Long COVID|Post-Acute Sequelae of COVID-19|COVID Long-Haul
Beth Israel Deaconess Medical Center
 October 2023 Not Applicable
NCT06110403 Recruiting
Copd
University Hospital Lille|AstraZeneca
 September 29 2023 Phase 1|Phase 2
NCT06012006 Not yet recruiting
COPD
Assistance Publique - Hôpitaux de Paris
 August 2023 Not Applicable

Chemical Information & Solubility

Molecular Weight 492.48 Formula

C19H32N4O11
CAS No. 53678-77-6 SDF --
Smiles CC(NC(=O)C(C)OC1C(O)C(CO)OC(O)C1NC(C)=O)C(=O)NC(CCC(O)=O)C(N)=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 98 mg/mL ( (198.99 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 98 mg/mL

Ethanol : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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