Home Proteases 3C-Like Protease inhibitor Merbromin

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Merbromin 3C-Like Protease inhibitor

Cat.No.S6872

Merbromin (Mercurochrome), a xanthene dye and an antibacterial agent, is the first of a series of antiseptics that contained mercury, also is a mixed-type inhibitor of 3CLpro due to its ability of increasing the KM and decreasing the Kcat of 3CLpro.
Merbromin 3C-Like Protease inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 752.68

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Quality Control

Batch: S687201 Water]100 mg/mL]false]DMSO]Insoluble]false]Ethanol]Insoluble]false Purity: 99%
99

Solubility

In vitro
Batch:

Water : 100 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 752.68 Formula

C20H10Br2HgO6.2Na

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 129-16-8 -- Storage of Stock Solutions

Synonyms Mercurochrome, Merbromine, Mercurocol, Sodium mercurescein, Asceptichrome, Supercrome, Brocasept, Cinfacromin Smiles C1=CC=C(C(=C1)C2=C3C=C(C(=O)C=C3OC4=C(C(=C(C=C24)Br)[O-])[Hg])Br)C(=O)[O-].O.[Na+].[Na+]

Mechanism of Action

Targets/IC50/Ki
3C-Like Protease
In vitro

Merbromin strongly inhibites the proteolytic activity of 3CLpro which is a promising target for developing anti-SARS-CoV-2 drugs. Michaelis-Menten kinetic analysis shows that this compound is a mixed-type inhibitor of 3CLpro, indicating by its ability of increasing the KM and decreasing the Kcat of 3CLpro. And 3CLpro possessed two binding sites for this chemical.
References

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