GC376

Catalog No.S0475

For research use only.

GC376 is a 3CLpro (3C-like protease) inhibitor with IC50 of ~ 1.11 μM for the PEDV 3CLpro. GC376 is active against the 3CLpro of multiple coronaviruses, including SARS-CoV.

GC376 Chemical Structure

CAS No. 1416992-39-6

Selleck's GC376 has been cited by 10 Publications

Purity & Quality Control

Choose Selective 3C-Like Protease Inhibitors

Biological Activity

Description GC376 is a 3CLpro (3C-like protease) inhibitor with IC50 of ~ 1.11 μM for the PEDV 3CLpro. GC376 is active against the 3CLpro of multiple coronaviruses, including SARS-CoV.
Targets
PEDV 3CLpro [1]
(Cell-free assay)
1.11 μM
In vitro

Molnupiravir and GC376 show a synergistic activity on SARS-CoV-2 at 48 h, and an additive activity on SARS-CoV-2 at 72 h.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HG23 Ml:wRY51cX[rcnHsJIF{e2G7 NUTRZ2R6SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhVm:{b4\pdpV{KGmwZnXjeIVlKGmwIHj1cYFvKEiJMkOgZ4VtdHNuIFXEOVAhRSByLkOg{txONg>? M3jjWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkG4O|E{Lz5{M{KxPFcyOzxxYU6=

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: Vero E6 cells
  • Concentrations: 0.21-16.7 μM
  • Incubation Time: 48 and 72 h
  • Method:

    Vero E6 cells (3000 cells/well) were seeded in 96-well clear flat-bottom plates and incubated at 37°C with 5% CO2 for 24 h. After incubation, cells were infected using a multiplicity of infection (MOI) of 0.1. SARS-CoV-2 was allowed to adsorb for one hour at 37°C. Subsequently, virus inoculum was removed, and cells were overlaid with media containing 3-fold serial dilutions of molnupiravir (0.62-50 μM), nirmatrelvir (0.62-50 μM) and GC376 (0.21-16.7 μM). Negative controls (compounds alone), infected positive controls (SARS-CoV-2 alone) and mock-infected cells were included in each plate. Plates were incubated at 37°C with 5% CO2 for 48 and 72 h and then, cell viability was measured using an MTT reduction assay.

    (Data sourced from selleck products)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 507.53
Formula

C21H30N3NaO8S

CAS No. 1416992-39-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)CC(C(=O)NC(CC1CCNC1=O)C(O)S(=O)(=O)[O-])NC(=O)OCC2=CC=CC=C2.[Na+]

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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