Home Cell Cycle Mad2 inhibitor M2I-1 For research use only.

M2I-1

M2I-1 is an inhibitor of Mad2 (mitotic arrest deficient 2) targeting the binding of Mad2 to Cdc20, an essential protein-protein interaction (PPI) within the SAC (spindle assembly checkpoint).

M2I-1 Chemical Structure

M2I-1 Chemical Structure

CAS: 312271-03-7

Purity & Quality Control

Batch: S018701 DMSO] 80 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.62%
99.62

M2I-1 Related Products

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Biological Activity

Description M2I-1 is an inhibitor of Mad2 (mitotic arrest deficient 2) targeting the binding of Mad2 to Cdc20, an essential protein-protein interaction (PPI) within the SAC (spindle assembly checkpoint).
Targets
Mad2 [1]
In vitro
In vitro

M2I-1, the first small molecule inhibitor targeting the binding of Mad2 to Cdc20, is an essential protein−protein interaction (PPI) within the SAC.[1]
Cell Research Cell lines HeLa cells
Concentrations 25, 50 and 75 μM
Incubation Time --
Method

HeLa cells were treated with 0.5 μM taxol and different concentrations of M2I-1 or DMSO as a solvent control.

Chemical Information & Solubility

Molecular Weight 404.48 Formula

C19H24N4O4S
CAS No. 312271-03-7 SDF --
Smiles CC(C)CN(CC(C)C)C1=C(C=C(C=C1)C=C2C(=O)NC(=S)NC2=O)[N+](=O)[O-]
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 80 mg/mL ( (197.78 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

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Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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