Home Proteases Glutaminase antagonist JHU-083 For research use only.

JHU-083

Synonyms: Ethyl 2-(2-Amino-4-methylpentanamido)-DON

JHU-083 (Ethyl 2-(2-Amino-4-methylpentanamido)-DON) is a novel prodrug of DON.JHU-083 selectively blocks glutaminase activity in brain CD11b+ cells and prevents depression-associated behaviors induced by chronic social defeat stress.Solutions are unstable and should be fresh-prepared.

JHU-083 Chemical Structure

JHU-083 Chemical Structure

CAS: 1998725-11-3

Selleck's JHU-083 has been cited by 1 publication

Purity & Quality Control

Batch: S889101 DMSO] 62 mg/mL] false] Ethanol] 62 mg/mL] false] Water] 3 mg/mL] true Purity: 99.80%
99.80

JHU-083 Related Products

Choose Selective Glutaminase Inhibitors

Biological Activity

Description JHU-083 (Ethyl 2-(2-Amino-4-methylpentanamido)-DON) is a novel prodrug of DON.JHU-083 selectively blocks glutaminase activity in brain CD11b+ cells and prevents depression-associated behaviors induced by chronic social defeat stress.Solutions are unstable and should be fresh-prepared.
Targets
Glutaminase [1]
In vitro
In vitro

JHU-083 selectively inhibits T-cell proliferation and decreases T-cell activation, with no effect on DCs.[2]
Cell Research Cell lines CD4+ T cells
Concentrations 1 μM, 3 μM, 10 μM, 30 μM, or 100 μM
Incubation Time 72h
Method

150,000 CD4+ T cells are plated with 20 μM eFlur450 cell proliferation dye, 2 μg/mL α-CD28, and ±JHU-083 in 100 μM complete RPMI. Every 24 hours, cells are treated with 20 μM media ±JHU-083.
In Vivo
In vivo

In vivo, orally administered JHU-083 significantly decreases the experimental autoimmune encephalomyelitis(EAE) severity in both prevention and treatment paradigms and reverses EAE-induced cognitive impairment.[2]
Animal Research Animal Models C57BL/6J mice
Dosages 1.83mg/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 312.36 Formula

C14H24N4O4
CAS No. 1998725-11-3 SDF --
Smiles CCOC(=O)C(CCC(=O)C=[N+]=[N-])NC(=O)C(CC(C)C)N
Storage (From the date of receipt) 3 years -20°C(in the dark) powder Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.

In vitro
Batch:

DMSO : 62 mg/mL ( (198.48 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 62 mg/mL

Water : 3 mg/mL


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In vivo
Batch:

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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