JHU-083

Catalog No.S8891 Synonyms: Ethyl 2-(2-Amino-4-methylpentanamido)-DON

For research use only.

JHU-083 (Ethyl 2-(2-Amino-4-methylpentanamido)-DON) is a novel prodrug of DON.JHU-083 selectively blocks glutaminase activity in brain CD11b+ cells and prevents depression-associated behaviors induced by chronic social defeat stress.Solutions are best fresh-prepared.

JHU-083 Chemical Structure

CAS No. 1998725-11-3

Purity & Quality Control

Choose Selective Glutaminase Inhibitors

Biological Activity

Description JHU-083 (Ethyl 2-(2-Amino-4-methylpentanamido)-DON) is a novel prodrug of DON.JHU-083 selectively blocks glutaminase activity in brain CD11b+ cells and prevents depression-associated behaviors induced by chronic social defeat stress.Solutions are best fresh-prepared.
Targets
Glutaminase [1]
()
In vitro

JHU-083 selectively inhibits T-cell proliferation and decreases T-cell activation, with no effect on DCs.[2]

In vivo

In vivo, orally administered JHU-083 significantly decreases the experimental autoimmune encephalomyelitis(EAE) severity in both prevention and treatment paradigms and reverses EAE-induced cognitive impairment.[2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: CD4+ T cells
  • Concentrations: 1 μM, 3 μM, 10 μM, 30 μM, or 100 μM
  • Incubation Time: 72h
  • Method:

    150,000 CD4+ T cells are plated with 20 μM eFlur450 cell proliferation dye, 2 μg/mL α-CD28, and ±JHU-083 in 100 μM complete RPMI. Every 24 hours, cells are treated with 20 μM media ±JHU-083.

Animal Research:

[2]

  • Animal Models: C57BL/6J mice
  • Dosages: 1.83mg/kg
  • Administration: oral

Solubility (25°C)

In vitro

DMSO 62 mg/mL
(198.48 mM)
Water 62 mg/mL
(198.48 mM)
Ethanol 62 mg/mL
(198.48 mM)

Chemical Information

Molecular Weight 312.36
Formula

C14H24N4O4

CAS No. 1998725-11-3
Storage 3 years -20°C(in the dark) powder
Smiles CCOC(=O)C(CCC(=O)C=[N+]=[N-])NC(=O)C(CC(C)C)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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