6-Diazo-5-oxo-L-norleucine

Catalog No.S8620 Synonyms: Diazooxonorleucine, 6-Diazo-5-oxo-L-nor-Leucine, L-6-Diazo-5-oxonorleucine, DON

For research use only.

6-Diazo-5-oxo-L-norleucine (Diazooxonorleucine, L-6-Diazo-5-oxonorleucine, DON), an antibiotic isolated from Streptomyces, is a glutaminase antagonist with IC50 of ~1  mM for cKGA (kidney-type glutaminase). 6-Diazo-5-oxo-L-norleucine exhibits analgesic, antibacterial, antiviral and anticancer properties.

6-Diazo-5-oxo-L-norleucine Chemical Structure

CAS No. 157-03-9

Selleck's 6-Diazo-5-oxo-L-norleucine has been cited by 3 Publications

Purity & Quality Control

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Biological Activity

Description 6-Diazo-5-oxo-L-norleucine (Diazooxonorleucine, L-6-Diazo-5-oxonorleucine, DON), an antibiotic isolated from Streptomyces, is a glutaminase antagonist with IC50 of ~1  mM for cKGA (kidney-type glutaminase). 6-Diazo-5-oxo-L-norleucine exhibits analgesic, antibacterial, antiviral and anticancer properties.
Targets
cKGA [1]
(Cell-free assay)
1 mM
In vitro

6-Diazo-5-oxo-L-norleucine appears to be positive in the in vitro chromosome aberration assay that it demonstrates dose dependent statistically positive increase in structural aberrations at 4 and 20 hour exposure without S9 and also at 4 hour exposure with S9.[2]

In vivo

6-Diazo-5-oxo-L-norleucine is evaluated for its in vivo clastogenic activity by detecting micronuclei in polychromatic erythrocyte (PCE) cells in bone marrow collected from the male mice dosed intravenously with 500, 100, 10, 1 and 0.1 mg/kg at 24 and 48 hour post dose. The in vivo micronucleus assay reveals a statistically positive response for micronucleus formation at 500, 100 and 10 mg/kg at 24 and 48 hour post dose.[2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: Chinese hamster ovary (CHO) cells
  • Concentrations: 0.1712 μg/mL to 1712 μg/mL
  • Incubation Time: 4 h, 20 h
  • Method:

    CHO cells are exposed to vehicle alone and to nine dose levels of test article, ranging from 0.1712 to 1712 μg/mL in the absence or presence of an S9 reaction mixture.

  • (Only for Reference)
Animal Research:

[2]

  • Animal Models: 6-week-old male ICR mice
  • Dosages: 500 mg/kg, 100 mg/kg, 10 mg/kg, 1 mg/kg, 0.1 mg/kg
  • Administration: IV
  • (Only for Reference)

Solubility (25°C)

In vitro

Water 34 mg/mL
(198.65 mM)
DMSO 7 mg/mL
(40.89 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 171.15
Formula

C6H9N3O3

CAS No. 157-03-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C(CC(=O)C=[N+]=[N-])C(C(=O)O)N

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05012462 Not yet recruiting Device: CM-1500 Fluid Loss Zynex Monitoring Solutions September 8 2021 Not Applicable
NCT04570969 Recruiting Procedure: Erector spinae plane block Liver Transplant Disorder Vinmec Healthcare System November 1 2020 Not Applicable
NCT04876794 Recruiting Device: ABLE Exoskeleton Spinal Cord Injuries ABLE Human Motion S.L.|Institut Guttmann|Heidelberg University Hospital Spinal Cord Injury Center November 3 2020 Not Applicable
NCT04516343 Recruiting Device: Robotic Ankle Assist Device (RAAD) Cerebral Palsy BiOMOTUM Inc.|Northern Arizona University|Gillette Children''s Specialty Healthcare|Shirley Ryan AbilityLab|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) November 1 2020 Phase 1
NCT04565535 Not yet recruiting Behavioral: Novel donor lifestyle optimisation protocol Liver Regeneration Institute of Liver and Biliary Sciences India October 1 2020 Not Applicable
NCT04448431 Recruiting Drug: Vortioxetine|Drug: Desvenlafaxine Major Depressive Disorder H. Lundbeck A/S June 18 2020 Phase 4

(data from https://clinicaltrials.gov, updated on 2021-09-06)

Tech Support

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