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DIM-C-pPhOH Nur77 antagonist

Name: DIM-C-pPhOH
Brand: Selleck Chemicals
SKU: S6799
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S6799

DIM-C-pPhOH (C-DIM8, CDIM8) is a nuclear receptor 4A1 (NR4A1)(Nur77) antagonist. This compound inhibits tumor growth and mTOR signaling, induces apoptosis and cellular stress. It reduces cell proliferation with IC50 of 13.6 μM and 13.0 μM for ACHN cells and 786-O cells, respectively.

Chemical Structure

Molecular Weight: 338.40

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Quality Control

Batch: Purity: 99.86%

99.86

Related Targets	CDK HSP PD-1/PD-L1 ROCK Wee1 DNA/RNA Synthesis Microtubule Associated Ras KRas Aurora Kinase
Other Nur77 Inhibitors	Cytosporone B (Csn-B) C-DIM5 DIM-C-pPhCO2Me

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
MCF7	Anticancer assay	2 uM	24 hrs	Anticancer activity against human MCF7 cells assessed as cell death at 2 uM after 24 hrs by MTT assay	22104151
MCF7	Function assay	2 uM	24 hrs	Inhibition of tubulin polymerization in human MCF7 cells at 2 uM after 24 hrs by FITC-conjugated immunostaining assay	22104151
MCF7	Function assay	2 uM	24 hrs	Inhibition of HDAC1 protein expression in human MCF7 cells at 2 uM after 24 hrs by Western blotting	22104151
MCF7	Function assay	2 uM	24 hrs	Inhibition of HDAC2 protein expression in human MCF7 cells at 2 uM after 24 hrs by Western blotting	22104151
MCF7	Function assay	2 uM	24 hrs	Inhibition of HDAC3 protein expression in human MCF7 cells at 2 uM after 24 hrs by Western blotting	22104151
MCF7	Function assay	2 uM	24 hrs	Inhibition of HDAC8 protein expression in human MCF7 cells at 2 uM after 24 hrs by Western blotting	22104151
MCF7	Function assay	8 uM	24 hrs	Induction of apoptosis in human MCF7 cells assessed as PARP-cleavage at 8 uM after 24 hrs by Western blot analysis	22104151
MCF7	Function assay	8 uM	24 hrs	Induction of apoptosis in human MCF7 cells assessed as caspase-7 activation at 8 uM after 24 hrs by Western blot analysis	22104151
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 200 mg/mL (591.01 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 68 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.500mg/ml (10.34mM)	Taking the 1 mL working solution as an example, add 50 μL of 70 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (14.78mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	338.40	Formula	C₂₃H₁₈N₂O	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	151358-47-3	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	C-DIM8, CDIM8			Smiles	C1=CC=C2C(=C1)C(=CN2)C(C3=CC=C(C=C3)O)C4=CNC5=CC=CC=C54

Mechanism of Action

Targets/IC₅₀/Ki	Nur77 (786-O cells based assay) 13.0 μM Nur77 (ACHN cells based assay) 13.6 μM
References	[1] https://pubmed.ncbi.nlm.nih.gov/26035713/

Tech Support

Handling Instructions

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