Caerulein (FI-6934)

Catalog No.S9690 Synonyms: Ceruletide, Cerulein

For research use only.

Caerulein (Ceruletide, Cerulein, FI-6934), a cholecystokinin (CCK) receptor agonist, is a safe and effective cholecystokinetic agent and small bowel and exocrine pancreatic stimulant.

Caerulein (FI-6934) Chemical Structure

CAS No. 17650-98-5

Purity & Quality Control

Choose Selective CCK receptor Inhibitors

Biological Activity

Description Caerulein (Ceruletide, Cerulein, FI-6934), a cholecystokinin (CCK) receptor agonist, is a safe and effective cholecystokinetic agent and small bowel and exocrine pancreatic stimulant.
Targets
CCK receptor [2]
In vitro

Incubation of isolated acinar cells with caerulein inhibits growth hormone secretagogue receptor (GHS-R) and Ghrelin (GHRL) expression at the level of mRNA and protein in those cells.[3]

In vivo

To study the effects of cholecystokinin receptor agonists or antagonists, 10 injection of morphine for dependence and a dose of 5 mg/kg of naloxone for withdrawal induction are employed. Low doses of the cholecystokinin agonists caerulein (0.00001 and 0.0001 mg/kg) elicits reduction of the nalaxone-induced jumping. The inhibition of jumping induced by caerulein is reduced with the selective cholecystokinin antagonists MK-329 and L-365,260. It is concluded that cholecystokinin mechanism(s) may be involved in morphine dependence, that the agonists may act on a presynaptic receptors.[2]

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: Pancreatic acinar cells
  • Concentrations: 0.01 μM
  • Incubation Time: 5 h
  • Method:

    Pancreatic acinar cells are isolated by collagenase digestion of pancreases obtained from the animals with intact SN or CDSN. 48 h earlier, during in vivo study, rats receives i.p. saline or GHRL. After isolation, pancreatic acinar cells are incubated in caerulein-free or caerulein containing solution. Caerulein concentration is 10-8 M, incubation time is 5 h.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 1352.40
Formula

C58H73N13O21S2

CAS No. 17650-98-5
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Caerulein (FI-6934) | Caerulein (FI-6934) supplier | purchase Caerulein (FI-6934) | Caerulein (FI-6934) cost | Caerulein (FI-6934) manufacturer | order Caerulein (FI-6934) | Caerulein (FI-6934) distributor