PD184352 (CI-1040)

Catalog No.S1020

For research use only.

PD184352 (CI-1040) is an ATP non-competitive MEK1/2 inhibitor with IC50 of 17 nM in cell-based assays, 100-fold more selective for MEK1/2 than MEK5. PD184352 (CI-1040) selectively induces apoptosis. Phase 2.

PD184352 (CI-1040) Chemical Structure

CAS No. 212631-79-3

Selleck's PD184352 (CI-1040) has been cited by 128 publications

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Biological Activity

Description PD184352 (CI-1040) is an ATP non-competitive MEK1/2 inhibitor with IC50 of 17 nM in cell-based assays, 100-fold more selective for MEK1/2 than MEK5. PD184352 (CI-1040) selectively induces apoptosis. Phase 2.
Features First MEK inhibitor to begin clinical development.
Targets
MEK1 [1]
(Cell-free assay)
MEK2 [1]
(Cell-free assay)
17 nM 17 nM
In vitro

CI-1040 treatment produces a reduction of pMAPK levels in multiple tumor cells including Colon 26, BX-PC3 pancreatic, A431 cervical, HT-29 colon, ZR-25-1 breast and SKOV-3 ovarian carcinomas. CI-1040 treatment doesn't inhibit the phosphorylation of Jun kinase, p38 kinase or Akt, indicating CI-1040 specifically targets MEK. Inhibition of MAPK activation by CI-1040 prevents cell cycle progression and induces a G1 block. [1] The IC50 for inhibition of MEK1 by CI-1040 is 0.3 μM, 15-fold higher than the concentration required to inhibit the EGF-induced activation of ERK2 in Swiss 3T3 cells. These results indicate CI-1040 exerts its effects on cells by suppressing the activation of MKK1, and not by blocking its activity. 2 nM PD184352 inhibits the activation of MKK1 in Swiss 3T3 cells by 50%, while over 100-fold concentration of CI-1040 inhibits MEK1 in vitro. PD184352 also inhibits the Raf-catalysed phosphorylation of MEK1 without any effect on the Raf-catalysed phosphorylation of myelin basic protein. [2] CI-1040 inhibits 86% of papillary thyroid carcinoma (PTC) cell growth with the RET/PTC1 rearrangement at 10μM compared with cells treated with DMSO only. CI-1040 shows potent inhibition to PTC cells (BRAF mutation) with GI50 of 52 nM, but low activity to RET/PTC1 rearrangement type with GI50 of 1.1 μM. [3] A recent research indicates CI-1040 increases the apoptotic effect of BMS-214662 in a CML blast crisis cell line, K562, and in primary chronic phase CD34+ CML cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC-3 NX2zfFRoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTJ2LkC1NlYh|ryP MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
VM-CUB-1 NXntWHo2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGjYcldKSzVyPUKzMlc{PDFizszN NXjKfIlwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
LC-2-ad NYTGfXd6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTJ|Lk[xOFch|ryP MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
A704 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjwTWM2OD1{Mz62NVA{KM7:TR?= MoThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
DMS-114 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjXTWM2OD1{Mz61O|Y2KM7:TR?= M2niWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
EKVX M{HINWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrvTWM2OD1{Mz6zPVA6KM7:TR?= M1vPfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
SNU-449 MnK3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIPqNIVKSzVyPUKzMlMzQSEQvF2= NGrWVlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
HCC1806 M3iyO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2KyOWlEPTB;MkOuNFU5OyEQvF2= NWTHUWZoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
CaR-1 NYLhe2k4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTJ|LkCyNVMh|ryP MmjrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
NCI-H441 M1\OfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIjCVWRKSzVyPUKyMlU3QDlizszN M2DQR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
TI-73 NESyUY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTJ{LkOyPEDPxE1? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
NCI-H1755 NFHRZmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrsTWM2OD1{MT62O|QyKM7:TR?= M3HsRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
A498 NIq1U4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTJzLkWzNVIh|ryP M3fQS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
CFPAC-1 NVS5UGFJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHlTWM2OD1{MT6zPFU5KM7:TR?= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
ChaGo-K-1 Ml;JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzDTWM2OD1{MT6zOlI4KM7:TR?= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
KP-4 MnvmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rsT2lEPTB;MkGuNVE6PiEQvF2= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
Becker M3PvSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTJzLkGxNVgh|ryP NF3kXFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
UMC-11 NEjLdmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPRTWM2OD1{MD65NVY1KM7:TR?= NVrpb2RRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
TE-6 NWPnVXZyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojhTWM2OD1{MD64NFY4KM7:TR?= NIT6RpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
SF539 M2Hpe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NED4bJVKSzVyPUKwMlUyPzJizszN MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
FTC-133 M{H4emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;GTWM2OD1{MD60O|A{KM7:TR?= NXjvdmg2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
NCI-H226 M4myS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTJyLkSzPVkh|ryP M1fD[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
NCI-H1048 M2PPb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;ITWM2OD1{MD6zNlY3KM7:TR?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
Calu-3 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHn0OlNKSzVyPUG5MlQzOzdizszN Mn\hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
SF126 NFjSZ4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPySFhKSzVyPUG5MlM2ODJizszN M4G0TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
NTERA-S-cl-D1 NIDpSFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zyXmlEPTB;MUmuNlAxPyEQvF2= NFvIOWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
SW1573 MnjjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTF7LkG5Olkh|ryP NYfLVGZDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
SNU-387 NFfsWJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1q3T2lEPTB;MUmuNVg5QSEQvF2= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
ETK-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnVcIZCUUN3ME2xPU4yQDJ5IN88US=> NUW5[m1kRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
HuO9 Mor6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTF7LkGzO|kh|ryP MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
RO82-W-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;F[WlEPTB;MUiuPFI5QCEQvF2= MljPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
D-502MG MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnkTWM2OD1zOD64NVM6KM7:TR?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
LS-123 NUnCU5JET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTF6Lke5NFEh|ryP MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
OC-314 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrJR|UxRTF6LkezPFQh|ryP NVzVfXhERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
Ramos-2G6-4C10 NIThRVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jue2lEPTB;MUiuOVkzPyEQvF2= MnTGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
SW962 M4LKOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGrybGJKSzVyPUG4MlU4ODJizszN M1PDe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
SW982 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\lW2lEPTB;MUiuOVY1QSEQvF2= NYK0R4tuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
KYSE-410 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmHVTWM2OD1zOD60OVc4KM7:TR?= MoTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
HT-3 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTF6LkO5O|ch|ryP M1POblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
CAL-85-1 NFvkdZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHzkT4VKSzVyPUG4MlE5ODlizszN M2rWWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
BB30-HNC MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrtPWZIUUN3ME2xPE4xOzF6IN88US=> NEHReJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
D-247MG NX;IeJVyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\kZ|RKSzVyPUG3MlkzPDdizszN M2LzelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
SCC-15 MmS2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTlcIhKSzVyPUG3Mlg1PzNizszN NIX5dJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
BB49-HNC M4npfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HlZmlEPTB;MUeuOVY5PiEQvF2= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
KYSE-70 MnX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTF5LkK4PUDPxE1? NEHzNlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
NKM-1 NWrpVHo2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTF5LkCxPFkh|ryP MkfiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
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NCI-H1563 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mly0TWM2OD1zNT6zOFkh|ryP NUnVRm5QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
AM-38 M364dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3W1emlEPTB;MUWuNlcyPyEQvF2= NFXYUmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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OVCAR-4 MnvqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rodmlEPTB;MUWuNVQzPCEQvF2= NFq3flA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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NCI-H661 M2fBOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTF2Lkm1PUDPxE1? MoHYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
LB831-BLC NUnKSpJYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2LYSWlEPTB;MUSuPFk{PCEQvF2= M3TvN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
BT-549 MlvuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rBfmlEPTB;MUSuPFI5PCEQvF2= NHK3b|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
NCI-H2347 M33NW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFnqPGdKSzVyPUG0MlgzOyEQvF2= NFfnW3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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BALL-1 M{DTXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3uWVRKSzVyPUG0MlQ3PjVizszN M2jmO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
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NB17 NGrnZY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkX2TWM2OD1zND6wPFUh|ryP MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
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SF268 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmDqTWM2OD1zMz62N|I3KM7:TR?= M4HuOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
NCI-H2009 NYL4R5d[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTF|LkW5NVkh|ryP M2nqd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
CAPAN-1 MlnQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmOzTWM2OD1zMz61OVE1KM7:TR?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
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OAW-42 Mn\yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTF|LkG5N|ch|ryP MmDvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
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CAL-39 NHrJeotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfXTWM2OD1{Lki5PVk6KM7:TR?= MlPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
LB2241-RCC Mn7JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnrBTWM2OD1{Lki1PFk{KM7:TR?= NFr1VoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
COLO-792 Mn7HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPlbGVUUUN3ME2yMlg1ODJ5IN88US=> NI\sU5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
HCE-T NEj0VW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M163SWlEPTB;Mj64N|I3PCEQvF2= NWnPfnNKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
BFTC-905 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3q5TmlEPTB;Mj64NlU{KM7:TR?= NFzp[YE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
CHL-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\odFNKSzVyPUKuPFA4QDVizszN NF\BbXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
L-363 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmWzTWM2OD1{Lke0N|ch|ryP MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
HCC2218 M2nOVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\FdJZRUUN3ME2yMlY1Pzd6IN88US=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
DB MkTQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzyTWM2OD1{LkW4O|g5KM7:TR?= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
EW-13 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXGTWM2OD1{LkW2N|M6KM7:TR?= NIKzfFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
KNS-62 MmqxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTJwNUGxOlQh|ryP NVS0Rm14RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
RD M4G2Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{fzZ2lEPTB;Mj60OFczOyEQvF2= MnXjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
BB65-RCC NUnOWZN4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV73dFRYUUN3ME2yMlQxPjd2IN88US=> M4\vOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
CAL-27 Mn\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTJwM{myN|Yh|ryP MnPXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
DOK M{npZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3j6bWlEPTB;Mj6zOVc4OyEQvF2= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
NB7 MlXsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4XNW2lEPTB;Mj6zOFA5PiEQvF2= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
A2058 NVLJV4hLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\GTWM2OD1{LkG5Nlc6KM7:TR?= NVXLTXU3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
HN MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzPTWM2OD1{LkG2Nlc5KM7:TR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
MZ2-MEL MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI[1dW5KSzVyPUKuNVEyOjdizszN NGHQPFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
EM-2 M1Oz[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlLsTWM2OD1{LkC3NVk5KM7:TR?= M{[2dFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
HCC70 NFz4[olIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1v6fGlEPTB;Mj6wNlQzPiEQvF2= NYHqOo5NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
RXF393 NXvVRWVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnfzTWM2OD1zLkm3PFg4KM7:TR?= NIrQTmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
LCLC-97TM1 MmHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWX0cZE6UUN3ME2xMlkzOjV4IN88US=> NUnwPXlmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
A375 M1PwXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnL1TWM2OD1zLki5O|E1KM7:TR?= NGHDUJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
BPH-1 NYfk[nJPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDwTWM2OD1zLki5OVkyKM7:TR?= M2L2fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
LOXIMVI Mm[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXjc2lxUUN3ME2xMlg6PDJ5IN88US=> M3jtNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
NCI-H292 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfVfHNKUUN3ME2xMlg3Pjl7IN88US=> NWqzPWJJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
NB69 M4HvOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYL6fnFKUUN3ME2xMlc5PDZ7IN88US=> NIXJ[lQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
OVCAR-5 MmXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7Ge4NKSzVyPUGuO|c3QDdizszN MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
COLO-829 MmnhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3TC[2lEPTB;MT62NlI{OiEQvF2= MnLVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
RT-112 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HRWWlEPTB;MT61PFk2OiEQvF2= M3z3TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
SK-MEL-2 MmTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITKSFVKSzVyPUGuOVc1PTlizszN MkPVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
HD-MY-Z NUjSVXJtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTFwNUezO|Yh|ryP NILNV2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
A4-Fuk MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV61fXNLUUN3ME2xMlU4ODR3IN88US=> M3\ZVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
SW1116 Mlz2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkDSTWM2OD1zLkW2N|k5KM7:TR?= NXvqdZVORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
NCI-H1437 MnvuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mke0TWM2OD1zLkW1Nlc2KM7:TR?= M4e0RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
ML-2 NX3UVmZ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn\uTWM2OD1zLkWyPFY5KM7:TR?= NYrxU4JGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
HO-1-N-1 NU\RWWJqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXS[XNyUUN3ME2xMlUyPjN2IN88US=> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
LoVo M{n3SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;HN2lEPTB;MT60OlM6QSEQvF2= NVfTN485RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
LS-513 NFHzeppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTFwNEWxPVIh|ryP M2XnVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
BV-173 NHXkdoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTFwNESwNVUh|ryP M3jFNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
LS-411N M{nSfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2DtWmlEPTB;MT60NFUyPyEQvF2= NVe1O4lCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
SW1463 MoPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX\tSVNDUUN3ME2xMlM{PzJzIN88US=> NILYSW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
NCI-H2291 M37qbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIXq[mVKSzVyPUGuN|I2OzZizszN NG[4SHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
H-EMC-SS MnjSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;vT2lEPTB;MT6yPVYxQCEQvF2= M3nlVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
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Assay
Methods Test Index PMID
Western blot p-ERK1/2 / ERK1/2 ; p-ERK5 / ERK5 21762482
Growth inhibition assay Cell viability 29989268
In vivo Oral dosing of CI-1040 impairs the growth of colon tumor xenografts of mouse and human with a wide dose range of 48-200 mg/kg per dose, but not of P388 leukemia. [1] CI-1040 inhibits the tumor xenografts from PTC cells carrying a BRAF mutation with 31.3% reduction, carrying the RET/PTC1 rearrangement with 47.5% reduction than in untreated (vehicle) mice after 3 weeks of oral administration (300 mg/kg/d). No toxic effects are observed in any mice when they are treated with CI-1040. [2] Transient exposure of mammary tumors to CI-1040 and UCN-01 causes tumor cell death in vivo and prolonged suppression of tumor regrowth. Combined treatment with CI-1040 (25 mg/kg) and UCN-01 (0.1-0.2 mg/kg) significantly reduces MDA-MB-231, and largely abolishs MCF7 tumor growth in implanted athymic mice, while either single treatment has no significant activity. The drug combination leads to profound tumor cell death which correlates with a reduction in the phosphorylation of ERK1/2 and the immuno-reactivity of Ki67 and of CD31. [5]

Protocol (from reference)

Kinase Assay:

[2]

  • MEK1 Assay:

    MAP kinase is activated after phosphorylation by MEK; the activated MAP kinase subsequently phosphorylates myelin basic protein (MBP).Incorporation of 32P into myelin basic protein (MBP) is assayed in the presence of glutathione S-transferase (GST) fusion proteins containing the 44-kDa MAPK (GST-MAPK) or the 45-kDa MEK (GST-MEK1). Assays are conducted in 50μL of 50 mM Tris, pH 7.4/10 mM MgCl2 /2 mM EGTA/10 μM [γ-32P]ATP containing 10 μg of GST-MEK1, 0.5 μg of GST-MAPK, and 40 μg of MBP. After incubation at 30°C for 15 minutes, reactions are stopped by addition of Laemmli SDS sample buffer. Phosphorylated MBP is resolved by SDS/10% PAGE. This screening effort leads to the discovery of several small-molecule inhibitors of MEK, i.e. CI-1040. Experiments assessing the order of addition shows that CI-1040 directly inhibits MEK1 with a 50% inhibitory concentration (IC50) of 17 nM, without affecting the activity of MAPK.

Cell Research:

[1]

  • Cell lines: Colon 26 carcinoma cells
  • Concentrations: 0.1-10 μM
  • Incubation Time: 24 hours
  • Method:

    Cells are planted seeded in T-75 cm 2 flasks and treated the next day for 24 hous with either DMSO or CI-1040. Single-cell suspensions are collected, and pellets are fixed in ice-cold ethanol (70%) for 30 minutes. After centrifugation of the samples, propidium iodide (50 μg/mL) and RNase (30 units/mL) are added to the pellets for 20 minutes at 37 °C. After filtration, samples are analyzed by flow cytometry.

Animal Research:

[3]

  • Animal Models: PTC cells in athymic mice
  • Dosages: 150 mg/kg
  • Administration: Orally twice daily

Solubility (25°C)

In vitro

DMSO 96 mg/mL
(200.55 mM)
Ethanol 14 mg/mL
(29.24 mM)
Water Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol, pH 9
For best results, use promptly after mixing.

10 mg/mL

Chemical Information

Molecular Weight 478.67
Formula

C17H14ClF2IN2O2

CAS No. 212631-79-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CC1CONC(=O)C2=C(C(=C(C=C2)F)F)NC3=C(C=C(C=C3)I)Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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