Home Autophagy Autophagy inhibitor Nortriptyline hydrochloride

research use only

Nortriptyline hydrochloride Autophagy inhibitor

Cat.No.S3698

Nortriptyline hydrochloride (Desitriptyline, ELF-101, EN-7048, Desmethylamitriptyline) is the hydrochloride salt form of nortriptyline, a tricyclic antidepressant agent used for short-term treatment of various forms of depression.
Nortriptyline hydrochloride Autophagy inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 299.84

Jump to

Quality Control

Batch: S369801 DMSO]59 mg/mL]false]Water]59 mg/mL]false]Ethanol]59 mg/mL]false Purity: 99.97%
99.97

Solubility

In vitro
Batch:

DMSO : 59 mg/mL (196.77 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 59 mg/mL

Ethanol : 59 mg/mL

Molarity Calculator

Mass Concentration Volume Molecular Weight
Dilution Calculator Molecular Weight Calculator

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg
g
μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO
%
% Tween 80
% ddH2O
% DMSO
+
%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight 299.84 Formula

C19H21N.HCl

 Storage (From the date of receipt)
CAS No. 894-71-3 Download SDF Storage of Stock Solutions

Synonyms Desitriptyline HCl, ELF-101 hydrochloride, EN-7048 hydrochloride, Desmethylamitriptyline hydrochloride Smiles CNCCC=C1C2=CC=CC=C2CCC3=CC=CC=C31.Cl

Mechanism of Action

In vitro
Nortriptyline inhibits the vascular Kv channels in a concentration-dependent and state-independent manner independently of serotonin reuptake inhibition. It is a tricyclic antidepressant. At the cellular level, nortriptyline is inhibitory to many proteins and responses such as astroglial inwardly rectifying Kir4.1 channels, human neutrophil phagocytosis and oxidative burst, human cytochrome P-450 enzymes, opioid receptors, Ca2+-activated K+ channels, and priming of human neutrophils. Nortriptyline induces a significant [Ca2+]i rise and decreases viability in PC3 cells, MG63 human osteosarcoma, and renal tubular cells. Nortriptyline also exhibits anticancer activity in several different types of cells. For example,in human cutaneous melanoma cells,NTP has a half maximal inhibitory concentration(IC50) of 9 μM compared with 27 μM and 33 μM for clomipramine and amitriptyline, respectively. Nortriptyline induces cell cycle arrest and apoptosis in TCCSUP and MBT-2 cells.
In vivo
Nortriptyline inhibits tumor growth in mice inoculated with MBT-2 cells. Low concentrations of NTP (0.1-5 μM) confer neuroprotective effects, downregulate cytosolic phospholipase A2, and prevent mitochondrial depolarization with minimal toxicity in both astrocytes and mice. In contrast, higher concentrations of NTP (10-50 μM) have anti-tumor effects on human osteosarcoma and cutaneous melanoma cells.
References

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Signaling Pathway Map