Catalog No.S1908 Synonyms: SCH-13521
Molecular Weight(MW): 276.21
Flutamide is an antiandrogen drug, with its active metablolite binding at androgen receptor with Ki values of 55 nM, and primarily used to treat prostate cancer.
2 Customer Reviews
Active Stat5a/b increases nuclear levels of antiandrogen-liganded AR in PC-3 cells. PC-3 cells were infected with AdAR, AdPrlR and AdWTStat5a, serum-starved for 12 h, and pretreated with 1 nM DHT, 10 μM Bicalutamide (Bic), 10 μM Flutamide (Flu) or 10 μM MDV3100 (MDV) for 1 h before stimulation with 10 nM Prl for 30 min, where indicated. Immunostaining depicts DAPI, Stat5a/b, AR and merged imaging of Stat5a/b and AR.
Mol Cancer Ther, 2015, 14(3):713-26. . Flutamide purchased from Selleck.
Effects of flutamide on Cd-induced Cx43 protein level and cell proliferation. (A) AR and (B) Cx43 were analyzed throughWestern blot. Results are reported as mean ± SD fromthree replicates. (C) Effect of flutamide on Cd-induced cell proliferation at 48 h, determined through an Edu test. *P<0.05 and ** P <0.01 vs. DMSO group; #P< 0.05 and ##P <0.01 vs. DMSO + Cd. AR, androgen receptor; DMSO, dimethyl sulfoxide; NS, no statistical significance.
J Appl Toxicol, 2017, 37(8):933-942. Flutamide purchased from Selleck.
Purity & Quality Control
Choose Selective Androgen Receptor Inhibitors
|Description||Flutamide is an antiandrogen drug, with its active metablolite binding at androgen receptor with Ki values of 55 nM, and primarily used to treat prostate cancer.|
Flutamide (Eulexin) is an antiandrogen drug. Flutamide-OH, the active metabolite of flutamide, directly binds at rat anterior pituitary androgen receptor with Ki values of 55 nM.  Flutamide does not affect the proliferation of an androgen-sensitive clone of the mouse mammary carcinoma Shionogi SC-l 15 cells in culture, shows only antiandrogenic effect, but not androgenic effect.  Flutamide provides treatment for prostate cancer when used along with leuprolide. 
|In vivo||Flutamide causes a markedly reduction in rat ventral prostate weight from 319 mg to 245 mg. A combination of flutamide and LHRH agonist induces an additive effect with a decrease in prostate weight to 101 mg, and an marked drop in prostatic ODC activity. |
Androgen Receptor Assay:Aliquots of 100 μl cytosol are incubated at 0-4°C for 18 h with 100 μl of the indicated saturating concentration of [3H]T in the presence or absence of increasing concentrations of nonlabeled T, DHT, flutamide (FLU) or flutamide-OH (FLU-OH). At the end of the incubation, free and bound T are separated by the addition of 200 μl dextran-coated charcoal (1 % charcoal, 0.1% dextran T-70, 0.1% gelatin, 1.5 mM EDTA and 50 mM Tris (pH 7.4)) for 15 min before centrifugation at 2300 × g for another 15 min at 0-4°C. Aliquots (350 μl) of the supernatant are transferred to scintillation vials with 10 ml of an aqueous counting solution before counting in a Beckman LS 330 counter.
|In vitro||DMSO||55 mg/mL (199.12 mM)|
|Ethanol||55 mg/mL (199.12 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Clinical Trial Information
|NCT Number||Recruitment||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT02918968||Recruiting||Prostate Cancer||Astellas Pharma Inc|Medivation, Inc.||November 2016||Phase 4|
|NCT02742675||Recruiting||Prostate Cancer||Fudan University||March 2016||Phase 2|
|NCT02346578||Recruiting||Castration-resistant Prostate Cancer||Taro Iguchi, MD, PHD|Astellas Pharma Inc|Osaka City University||January 2015||--|
|NCT01889199||Recruiting||Polycystic Ovary Syndrome (PCOS)||University of California, Los Angeles|University of Wisconsin, Madison|Oregon Health and Science University||April 2013||Phase 2|
|NCT02341404||Completed||Prostate Cancer||Lidds AB|Uppsala University||May 2012||Phase 2|
|NCT01546987||Active, not recruiting||Prostate Cancer||Radiation Therapy Oncology Group|National Cancer Institute (NCI)|NRG Oncology||May 2012||Phase 3|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.