USP25/28 inhibitor AZ1

Catalog No.S8904

For research use only.

AZ1 is a potent and noncompetitive dual Inhibitors of the ubiquitin-specific protease (USP) 25/28 with IC50s of 0.62 μM and 0.7 μM in Ub-RH110 assay,respectively.

USP25/28 inhibitor AZ1 Chemical Structure

CAS No. 2165322-94-9

Purity & Quality Control

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Biological Activity

Description AZ1 is a potent and noncompetitive dual Inhibitors of the ubiquitin-specific protease (USP) 25/28 with IC50s of 0.62 μM and 0.7 μM in Ub-RH110 assay,respectively.
Targets
USP25 [1]
(Cell-free assay)
USP28 [1]
(Cell-free assay)
0.62 μM 0.7 μM
In vitro

AZ1 is able to reduce cell viability across a range of cancer cell lines with EC50 values typically clustered around 20 μM.[1]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 84 mg/mL
(198.95 mM)
Ethanol 84 mg/mL
(198.95 mM)
Water Insoluble

Chemical Information

Molecular Weight 422.21
Formula

C17H16BrF4NO2

CAS No. 2165322-94-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=C(C=C1COC2=C(C=C(C=C2)Br)CNCCO)C(F)(F)F)F

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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