SB 4 (Eticovo)

For research use only.

Catalog No.S0523

SB 4 (Eticovo) Chemical Structure

CAS No. 100874-08-6

SB 4 (Eticovo) is a potent and selective agonist of bone morphogenetic protein 4 (BMP4) signaling with EC50 of 74 nM. SB 4 (Eticovo) enhances canonical BMP signaling and activates SMAD-1/5/9 phosphorylation.

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Description SB 4 (Eticovo) is a potent and selective agonist of bone morphogenetic protein 4 (BMP4) signaling with EC50 of 74 nM. SB 4 (Eticovo) enhances canonical BMP signaling and activates SMAD-1/5/9 phosphorylation.
Targets
BMP4 [1]
(Cell-free assay)
74 nM(EC50)
Assay
Methods Test Index PMID
Western blot
p-SMAD-1/5/9 / t-SMAD-1 / β-Actin; 

PubMed: 30602563     


A, immunoblotting of whole cell protein lysates from BRE-Luc cells treated with either 2 ng/ml rhBMP4, 0.04% DMSO, or the top 12 BMP candidate agonists at 10 μm for 1 h in BRE-Luc cells. Membranes were probed with anti-p-SMAD-1/5/9 and anti-total-SMAD-1. β-Actin served as an additional loading control. B, quantitation of p-SMAD-1/5/9 protein levels as determined by densitometry of three independently derived Western blots. The p-SMAD-1/5/9 levels were normalized to total-SMAD-1. Final data are expressed as -fold change relative to the mean negative control signal, with error bars representing 1 S.D. (*, p < 0.05; **, p < 0.01; ****, p < 0.0001 relative to control). ANOVA, Dunnett's post hoc multiple comparisons test was used to determine significance.

p-SMAD-1/5/9 / t-SMAD-1 / β-Actin; 

PubMed: 30602563     


C, immunoblotting of lysates from serum-starved PRECs treated for 24 h with increasing concentrations of sb4 in serum-free medium. Total SMAD-1 and β-actin serve as loading controls. D, quantitation of p-SMAD-1/5/9 levels are shown. Statistical analyses of replicates were done as in B.

30602563

Protocol

Solubility (25°C)

In vitro DMSO 64 mg/mL (199.87 mM)
Water ˂1 mg/mL
Ethanol '''21 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 320.20
Formula

C14H10BrNOS

CAS No. 100874-08-6
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C2C(=C1)N=C(O2)SCC3=CC=C(C=C3)Br

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID