Name: SB 4
Brand: Selleck Chemicals
SKU: S0523
Rating: 5 (1 reviews)

SB 4 is a potent and selective agonist of bone morphogenetic protein 4 (BMP4) signaling with EC50 of 74 nM. SB 4 enhances canonical BMP signaling and activates SMAD-1/5/9 phosphorylation.

SB 4 Chemical Structure

CAS: 100874-08-6

Purity & Quality Control

Batch: S052301 DMSO] 64 mg/mL] false] Ethanol] 21 mg/mL] false] Water] ˂1 mg/mL] false Purity: 99.94%

99.94

SB 4 Related Products

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Signaling Pathway

TGF-beta/Smad Signaling Pathway

Choose Selective TGF-beta/Smad Inhibitors

Biological Activity

Description

SB 4 is a potent and selective agonist of bone morphogenetic protein 4 (BMP4) signaling with EC50 of 74 nM. SB 4 enhances canonical BMP signaling and activates SMAD-1/5/9 phosphorylation.

Targets

BMP4 ^[1]
(Cell-free assay)

74 nM(EC50)

In vitro
In vitro	BMP signaling agonist SB 4 induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions. SB 4 enhances the efficacy of signaling at each concentration of rhBMP4 tested. SB 4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4.^[1]
Cell Research	Cell lines	Primary mouse kidney epithelial cells (PRECs)
	Concentrations	0.05 μM-1 μM
	Incubation Time	0-1 h, 24 h
	Method	The BRE-Luc cells are stimulated with 2 ng/ml rhBMP4 for 1 h, after which the media are removed and replaced with fresh media containing either DMSO or 1 μm SB 4. p-SMAD-1/5/9 levels are then examined after 0, 5, 15, 30, 45, and 60 min of SB 4 treatment and compared with DMSO controls.
Experimental Result Images	Methods	Biomarkers	Images	PMID
Experimental Result Images	Western blot	p-SMAD-1/5/9 / t-SMAD-1 / β-Actin p-SMAD-1/5/9 / t-SMAD-1 / β-Actin		30602563

References

[1] Bradford STJ, Ranghini EJ, Grimley E, Lee PH, Dressler GR. High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds. J Biol Chem. 2019 Mar 1;294(9):3125-3136.

Chemical Information & Solubility

Molecular Weight	320.20	Formula	C₁₄H₁₀BrNOS
CAS No.	100874-08-6	SDF	--
Smiles	C1=CC=C2C(=C1)N=C(O2)SCC3=CC=C(C=C3)Br
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 64 mg/mL ( (199.87 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 21 mg/mL

Water : ˂1 mg/mL

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In vivo
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