Home Epigenetics ATF/CREB & c-Fos/c-Jun inhibitor compound 3i (666-15)

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compound 3i (666-15) ATF/CREB & c-Fos/c-Jun inhibitor

Cat.No.S8846

Compound 3i (666-15) is a potent and selective inhibitor of CREB-mediated gene transcription (IC50 = 0.081 ± 0.04 μM) and also potently inhibits cancer cell growth without harming normal cells.
compound 3i (666-15) ATF/CREB & c-Fos/c-Jun inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 620.52

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Quality Control

Batch: S884601 DMSO]100 mg/mL]false]Ethanol]3 mg/mL]false]Water]Insoluble]false Purity: 99.85%
99.85

Cell Culture, Treatment & Working Concentration

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDA-MB-468 Antiproliferative assay 72 hrs Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell growth after 72 hrs by MTT assay, GI50=0.046μM 26023867
MDA-MB-231 Antiproliferative assay 72 hrs Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth after 72 hrs by MTT assay, GI50=0.073μM 26023867
HEK293T Function assay Inhibition of CREB-mediated gene transcription in human HEK293T cells transfected with pCRE-RLuc by renilla luciferase reporter assay, IC50=0.081μM 26023867
HEK293T Function assay 30 mins Inhibition of CREB (unknown origin) expressed in HEK293T cells assessed as inhibition of forskolin-induced CREB transcription activity incubated for 30 mins by luciferase reporter gene assay, IC50=0.081μM 31765143
MCF7 Antiproliferative assay 72 hrs Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth after 72 hrs by MTT assay, GI50=0.31μM 26023867
A549 Antiproliferative assay 72 hrs Antiproliferative activity against human A549 cells assessed as inhibition of cell growth after 72 hrs by MTT assay, GI50=0.47μM 26023867
HEK293T Function assay 30 mins Inhibition of NFkappaB (unknown origin)-mediated gene transcription in human HEK293T cells transfected with an NFkappaB firefly luciferase reporter gene assessed as inhibition of TNFalpha-stimulated gene transcription pretreated for 30 mins measured after, IC50=5.29μM 26023867
HEK293T Function assay 50 nM 1 hr Inhibition of CREB-mediated gene transcription in human HEK293T cells assessed as inhibition of forskolin-stimulated Nurr1/NR4A2 transcription at 50 nM after 1 hr by qRT-PCT analysis 26023867
HEK293T Function assay > 1 uM 30 mins Inhibition of VP16/CREB (unknown origin) interaction in human HEK293T cells assessed as inhibition of forskolin-stimulated gene transcription at > 1 uM after 30 mins by CRE-RLuc complementation assay 26023867
HEK293T Function assay > 1 uM 30 mins Inhibition of P53 (unknown origin) transfected in human HEK293T cells assessed as inhibition of forskolin-stimulated gene transcription at > 1 uM after 30 mins by CRE-RLuc complementation assay 26023867
MDA-MB-468 Antitumor assay 10 mg/kg 5 weeks Antitumor activity against human MDA-MB-468 cells xenografted in BALB/c mouse assessed as reduction in tumor volume at 10 mg/kg, ip once a day, 5 days per week for 5 weeks relative to control 26023867
HEK293T Function assay 100 nM 1 hr Inhibition of CREB in HEK293T cells assessed as inhibition of Fsk-induced NR4A2 gene expression at 100 nM pre-incubated for 1 hr before Fsk stimulation for 45 mins by qRT-PCR analysis 31765143
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Solubility

In vitro
Batch:

DMSO : 100 mg/mL (161.15 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble

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In vivo
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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 620.52 Formula

C33H31Cl2N3O5

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 1433286-70-4 -- Storage of Stock Solutions

Synonyms N/A Smiles C1=CC=C2C=C(C(=CC2=C1)C(=O)NCCOC3=CC4=CC=CC=C4C=C3C(=O)NC5=C(C=C(C=C5)Cl)O)OCCCN.Cl

Mechanism of Action

Targets/IC50/Ki
CREB
81 nM
In vitro

Compound 3i (666-15) could potently inhibit cancer cell growth. In MDA-MB-231 and MDA-MB-468 cells, the GI50 for this compound is 73 and 46 nM, respectively. In A549 and MCF-7 cells, it exhibits robust activity as well with GI50 of 0.47 and 0.31 μM. It inhibits CREB’s transcription activity in living cells independent of direct CREB or CBP binding interaction.
In vivo

In an in vivo MDA-MB-468 xenograft model, compound 3i (666-15) completely suppresses tumor growth without overt toxicity. Preliminary toxicity studies show that intraperitoneal (ip) injection of 10 mg/kg of it is well tolerated in mice.
References

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