AZ505

Catalog No.S7042

For research use only.

AZ505 is a potent and selective inhibitor of SMYD2 with an IC50 of 0.12 μM.

AZ505 Chemical Structure

CAS No. 1035227-43-0

Purity & Quality Control

Choose Selective SMYD Inhibitors

Biological Activity

Description AZ505 is a potent and selective inhibitor of SMYD2 with an IC50 of 0.12 μM.
Targets
SMYD2 [1]
0.12 μM
In vitro

AZ505 inhibits the binding of SMYD2 to the miR-125b promoter region (based on chromatin immunoprecipitation assays) and suppresses clear cell renal cell carcinoma (ccRCC) cells migration and invasion by inhibiting the SMYD2/miR-125b/DKK3 pathway. <sup><a class="sref" href="#s_ref">[2]</a></sup>

In vivo

AZ505 treatment resulted in the decrease of the tumor sizes and weights in the renal cell carcinoma xenograft model mice. <sup><a class="sref" href="#s_ref">[2]</a></sup>

Protocol (from reference)

Cell Research:

<sup><a class="sref" href="#s_ref">[2]</a></sup>

  • Cell lines: OSRC-2cells, 786-O cells
  • Concentrations: 0-1.5 mM
  • Incubation Time: 12 h
  • Method:

    To evaluate the differentially expressed miRNAs in AZ505-treated and siSMYD2-transfected ccRCC cells relative to control cells, siSMYD-, AZ505-, and negative control-treated 786-O cells are subjected to miRNA microarray, using the miRCURY LNA Array system. And then ChIP is performed using the EZ-Chromatin Immunoprecipitation Kit.

Animal Research:

<sup><a class="sref" href="#s_ref">[2]</a></sup>

  • Animal Models: BALB/c nude mice bearing with 786-O cells
  • Dosages: 40 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 577.54
Formula

C29H38Cl2N4O4

CAS No. 1035227-43-0
Storage 3 years -20°C powder
2 years -80°C in solvent

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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