Pomalidomide

Catalog No.S1567 Synonyms: CC-4047

Pomalidomide Chemical Structure

Molecular Weight(MW): 273.24

Pomalidomide inhibits LPS-induced TNF-α release with IC50 of 13 nM in PBMCs.

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In DMSO USD 91 In stock
USD 70 In stock
USD 120 In stock
USD 370 In stock
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Cited by 19 Publications

3 Customer Reviews

  • MM.1S cells were cultured with Len (lenalidomide) or Pom (pomalidomide) for 48 h.

    Blood Cancer Journal, 2015, 5: e312. Pomalidomide purchased from Selleck.

    OPM2 cells stably expressing either NT or CRBN shRNA were seeded and incubated with pomalidomide at the indicated concentration, followed by MTT assay at day 3 after adding drugs. Each experimental condition was performed in triplicate and repeated at least once.

     

     

    Blood 2011 118, 4771-4779. Pomalidomide purchased from Selleck.

  • A) Quantitative determination of monopolar spindles in MM.1S cells treated with the vehicle (control), PD, F or PDF for 24h. Representative micrographs showing cells immunostained with anti-tubulin antibody (shown in green) to visualize microtubules and DAPI (blue) to evidence nuclei using confocal microcopy. Bar = 15 μm in lower magnification micrograph; bar = 5 μm in higher magnification insert). Data are presented as the mean ± SD of two independent experiments. Statistical significance was evaluated with Student’s t-test.

    Haematologica, 2017, 102(12):2113-2124. Pomalidomide purchased from Selleck.

Purity & Quality Control

Choose Selective TNF-alpha Inhibitors

Biological Activity

Description Pomalidomide inhibits LPS-induced TNF-α release with IC50 of 13 nM in PBMCs.
Features A derivative of thalidomide and up to 10,000 times more potent than thalidomide.
Targets
TNF-α [1]
(PBMCs)
13 nM
In vitro

Pomalidomide inhibits lipopolysaccharide (LPS) stimulated TNF-alpha release in human PBMC and in human whole blood with IC50 values of 13 nM and 25 nM, respectively. [1] Pomalidomide inhibits the growth of T regulatory cells which is stimulated by IL-2 with an IC50 of ~1 μM. [2] Treatment with Pomalidomide (6.4 nM-10 μM) increases the production of IL-2 in human peripheral blood T cells, and is slightly more potent in the CD4+ subset than in the CD8+ subset. Pomalidomide is significantly more potent than CC-5013 at elevating IL-2, IL-5, and IL-10 levels, but only slightly more potent than CC-5013 at elevating IFN-γ levels. Pomalidomide enhances SEE and Raji cells induced AP-1 transcriptional activity in Jurkat cells in a dose-dependent manner, with a maximal enhancement of 4-fold at 1 μM. [3] Exposure of Raji cells to various concentrations of Pomalidomide (2.5-40 μg/mL) for 48 hours leads to a significant decrease in cell proliferation and DNA synthesis. There is a reduction of ~40% compared to vehicle-treated controls. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MOLP-8 NYL4XXVJS3m2b4TvfIlkcXS7IFHzd4F6 M{\hN|ExKM7:TR?= NGfjcG8zPCCq NG\mU45xd3SnboTsfUBifWevZX70d{BlcXKnY4SgZY5lKGmwZHny[YN1KE2PIHPlcIwhc2mubHnu[{BjgSCVQWK= NXm3S4FoOjZ|M{iyO|M>
J-CD38 M13aUmN6fG:2b4jpZ4l1gSCDc4PhfS=> NUj0R3VoOTBizszN NF\KN5QzPCCq MonGdI91\W62bImgZZVodWWwdIOg[Ilz\WO2IHHu[EBqdmSrcnXjeEBOVSClZXzsJItqdGyrbnegZpkhW0GU M3fKOFI3OzN6Mkez
R-CD38 MlHxR5l1d3SxeHnjbZR6KEG|c3H5 MoOwNVAh|ryP MVGyOEBp MmXldI91\W62bImgZZVodWWwdIOg[Ilz\WO2IHHu[EBqdmSrcnXjeEBOVSClZXzsJItqdGyrbnegZpkhW0GU Ml7WNlY{Ozh{N{O=
BC-3 NWr0fYF6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7YRmEzOzlvMUK1NEBvVQ>? MkDmOUBl NYn3bGFqTE2VT9Mg NF\zeZdKSzVyPUGwO{BvVSxiaX7obYJqfHNiY3XscEBKSzVyPUGwO{BvVSxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? MmLGNlYyOTl7M{m=
BCBL-1 M3XETGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHFdldyOzlvMUK1NEBvVQ>? Ml7hOUBl NELZVGRFVVORwrC= NXLudng3UUN3ME23OEBvVSxiaX7obYJqfHNiY3XscEB3cWGkaXzpeJkh\G:|ZTDk[ZBmdmSnboTsfS=> MmnvNlYyOTl7M{m=
JSC-1 NYC1fGoyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkSzN|kuOTJ3MDDuUS=> MYK1JIQ> M2O3VmROW00EoB?= MkG0TWM2OD1|NDDuUUwhcW6qaXLpeJMh[2WubDD2bYFjcWyrdImg[I9{\SCmZYDlcoRmdnSueR?= Mk\hNlYyOTl7M{m=
VG-1 MkG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1ziUlM6NTF{NUCgcm0> MmjOOUBl MkDqSG1UV8Li NIOwbo5KSzVyPUGwNUBvVSxiaX7obYJqfHNiY3XscEB3cWGkaXzpeJkh\G:|ZTDk[ZBmdmSnboTsfS=> NU\lRXdUOjZzMUm5N|k>
UMPEL-1 NIr0emRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3hRYU{QS1zMkWwJI5O NELpb2U2KGR? MX;EUXNQyqB? MVrJR|UxRTN{IH7NMEBqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 NH[4UoYzPjFzOUmzPS=>
UMPEL-3 NHTtbGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPUO|VuOzlvMUK1NEBvVQ>? NYLBWGY3PSCm NXPlUmhuTE2VT9Mg Mor1TWM2OD1zMUGgcm0tKGmwaHnibZR{KGOnbHygeoli[mmuaYT5JIRwe2ViZHXw[Y5l\W62bIm= M3i5[lI3OTF7OUO5
BC-1 M{P1V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnacnE{QS1zMkWwJI5O NGrCNVA2KGR? M4XiVGROW00EoB?= M13kTmlEPTB;N{S0JI5ONCCrbnjpZol1eyClZXzsJJZq[WKrbHn0fUBld3OnIHTldIVv\GWwdHz5 Mm\ENlYyOTl7M{m=
BCP-1 M{LzT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvncJFGOzlvMUK1NEBvVQ>? MmrNOUBl MojPSG1UV8Li MkTMTWM2OD1|OU[gcm0tKGmwaHnibZR{KGOnbHygeoli[mmuaYT5JIRwe2ViZHXw[Y5l\W62bIm= MkH6NlYyOTl7M{m=
APK-1 M1XvSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUjy[mk{OzlvMUK1NEBvVQ>? M3;sTFUh\A>? MV3EUXNQyqB? MWLJR|UxRTJ{NjDuUUwhcW6qaXLpeJMh[2WubDD2bYFjcWyrdImg[I9{\SCmZYDlcoRmdnSueR?= MljUNlYyOTl7M{m=
RPMI8226  M17YSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELwW5QxNjBzLUWwJO69VQ>? M4rkSFQ5KGh? M1jZcWROW00EoB?= NXu5dIhtUUN3ME24JO69VQ>? MmL0NlYxQTd6N{K=
OPM2  M{\JfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHPNE4xOS13MDFOwG0> NGH4Vok1QCCq NHe4bmFFVVORwrC= M4HvV2lEPTB;MUCg{txO MkTYNlYxQTd6N{K=
RPMI8226  NV\CVmt[TnWwY4Tpc44hSXO|YYm= M1vMWFExKM7:TR?= MkX0OFghcA>? Ml;0SG1UV8Li Ml;Ed5Rz\W6pdHjlcpMh[3m2b4DsZZNucWNvboXjcIVieiC|aIX0eIxqdmdib3[gcXRQWiCjbnSgdE1uXE:UIIDyc5RmcW5? M1\CUFI3ODl5OEey
OPM2  NYfLW4cxTnWwY4Tpc44hSXO|YYm= M1rJbVExKM7:TR?= NUjpfWlTPDhiaB?= Ml7RSG1UV8Li MUjzeJJmdme2aHXud{BkgXSxcHzhd41q[y2wdXPs[YFzKHOqdYT0cIlv\yCxZjDtWG9TKGGwZDDwMY1VV1JicILveIVqdg>? NYXTVmc1OjZyOUe4O|I>
RPMI8226 MX7GeY5kfGmxbjDBd5NigQ>? NWXR[VJ6OC5zLUGwJO69VQ>? MUm0JIg> M2nkNWROW00EoB?= M3noNIlv[3KnYYPld{BXTUeIIH3SUmEh\XiycnXzd4lwdg>? NFHTT5gzPTB3M{m5NC=>
SH-SY5Y  M2jrWGFxd3C2b4Ppd{BCe3OjeR?= NGDCN3kzPcLizsznM41N MkPBNeKhcA>? M2rTcYNifXOnczDzeIF1cXO2aXPhcIx6KHOrZ37p[olk[W62IILl[JVkfGmxbjDpckBjd3SqIFPQSk0h[W6mIFPQSktEVS2rbnT1Z4VlKGGyb4D0c5Nqe8Li M2izNVI1QTd3Mke2
JJN3 Mn\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{XHO|AvOS1zMECg{txO NVnh[lNuPzJiaB?= MUDEUXNQ Mmn3bY5pcWKrdIOgZ4VtdCCpcn;3eIghe2yrZ3j0cJk> NWjRT2dpOjNzN{izO|g>
XG-1 M{Hsbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\TNE4yNTFyMDFOwG0> MnvVO|IhcA>? MojZSG1UVw>? M2nrN4lvcGmkaYTzJINmdGxiZ4Lve5Rp MYqyN|E4QDN5OB?=
CD138+  NXThW29NT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHTGUJQxNjFvMUCwJO69VQ>? MkfNO|IhcA>? NHTUVnVFVVOR NGn5Om1qdmirYnn0d{Bk\WyuIHfyc5d1cA>? M1jHWFI{OTd6M{e4
XG-1 MnnwSpVv[3Srb36gRZN{[Xl? Mkj1Nk8yODBizszN M{DzRlI1KGh? Mnj2SG1UVw>? MV;pcohq[mm2czDDR2w{N02LUD2x{tEhdVKQQTDlfJBz\XO|aX;u MlzlNlMyPzh|N{i=
U266 NWHMXG55T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWiwMlAyNTFyIN88US=> NV\ycJdRPDkkgJno MVLEUXNQ NYLCNIQxcW6qaXLpeJMh[2WubDDndo94fGhiZH;z[UBl\XCnbnTlcpRtgQ>? MXKyNlU2OjByOB?=
CRBN60 NUHOdog5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU[wMlAyNTFyIN88US=> NUfyZ3JjPDkkgJno MlPNSG1UVw>? M4P1dolvcGmkaYTzJINmdGxiZ4Lve5RpKGSxc3Wg[IVx\W6mZX70cJk> NEnoTHUzOjV3MkCwPC=>
CRNB75 NHn1b4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPTdpRLOC5yMT2xNEDPxE1? MX60PQKBkWh? Mm\RSG1UVw>? MVjpcohq[mm2czDj[YxtKGe{b4f0bEBld3OnIHTldIVv\GWwdHz5 NUW3[WR{OjJ3NUKwNFg>
MM.1S MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXiwMlAyNTFyIN88US=> NHjafWU1QOLCiXi= M4nYTWROW09? NUnxe5pve2mpbnnmbYNidnSueTDpcohq[mm2czDwdo9tcW[ncnH0bY9vKGG2IHPvcoNmdnS{YYTpc45{KGG|IHzve{BieyByLkCx{txO NWLjfHRFOjF|OEmzNlc>
OPM2 MkXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUCwMlAyNTFyIN88US=> MUe0PQKBkWh? Ml7YSG1UVw>? MYrzbYdvcW[rY3HueIx6KGmwaHnibZR{KHC{b3zp[oVz[XSrb36gZZQh[2:wY3XueJJifGmxboOgZZMhdG:5IHHzJFAvODIQvF2= MV6yNVM5QTN{Nx?=
MM.1S NYjuPZhGTnWwY4Tpc44hSXO|YYm= NYrBXocyOTBizszN MnHKO|IhcA>? MULEUXNQ MWDzbYdvcW[rY3HueIx6KGSnY4LlZZNmeyC2aHWgdJJwfGWrbjDs[ZZmdCCxZjDDM2VDWM7{IHnzc4Zwem2|wrC= M3GzWlIyOzh7M{K3
H929 MmKzSpVv[3Srb36gRZN{[Xl? MmfCNVAh|ryP NHnVRnY4OiCq MVHEUXNQ NIL4T|B{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczD0bIUheHKxdHXpckBt\X[nbDDv[kBEN0WEUN8yJIl{d2[xcn3zxsA> NFjiTIYzOTN6OUOyOy=>
OPM2 MV3GeY5kfGmxbjDBd5NigQ>? MVKxNEDPxE1? MmXuO|IhcA>? NUDafmxMTE2VTx?= NXS2V|h5e2mpbnnmbYNidnSueTDk[YNz\WG|ZYOgeIhmKHC{b4TlbY4hdGW4ZXygc4YhSy:HQmFOtkBqe2:ob4Ltd:Kh M{T0e|IyOzh7M{K3
CT26 Mor6SpVv[3Srb36gRZN{[Xl? M2f0blEwOTBizszN Mn\ZNlQhcA>? MVTy[YR2[2W|IITo[UBvfW2kZYLzJI9nKGyrdnWgZ49td26rZYRCpC=> MUixPVY{QDl5Nx?=

... Click to View More Cell Line Experimental Data

In vivo Pomalidomide enhances the antitumor effect of rituximab against B-cell lymphomas in severe combined immunodeficient mice. Administration of Pomalidomide in combination with rituximab, gives the mice a median survival period of 74 days compared with 58 days of CC5013/rituximab treatment and 45 days of rituximab nonotherapy. The synergistic effect of Pomalidomide and rituximab can be completely abrogated by depletion of NK cells, supporting the proposal that NK cell expansion is one mechanism by which Pomalidomide may augment rituximab antitumor activity. [4]

Protocol

Kinase Assay:[1]
+ Expand

Inhibition of TNF-α synthesis:

TNF-α inhibitory activity is measured in lipopolysacharide (LPS) stimulated PBMC. Pomalidomide is added to human PBMCs 1 hour prior to the addition of LPS (1 μg/mL) and incubation continued for an additional 18-20 hours. Supernatants are then harvested, and the concentration of TNF-α in the supernatants is determined by ELISA. The concentration of Pomalidomide that inhibits TNF- production by 50% (IC50) is calculated by nonlinear regression analysis. The human whole blood TNF- inhibition assay is run in a similar fashion to the PBMC assay except that heparinized fresh human whole blood is plated directly into microtiter plates.
Cell Research:[4]
+ Expand
  • Cell lines: Raji, SU-DHL-4 and SU-DHL-10 cell lines
  • Concentrations: Dissolved in DMSO, final concentrations 2.5-40 μg/mL
  • Incubation Time: 24 or 48 hours
  • Method: For assessment of cell apoptosis, Lymphoma cell lines are exposed to Pomalidomide (5 μg/mL) for 24 hours or 48 hours. The cells are stained with FITC-labeled Annexin V and propidium iodine. Cell apoptosis is analyzed by multicolor flow cytometric analysis using a fluorescence-activated cell sorter/FACStar Plus flow cytometer. Cells are scored as apoptotic if they are Annexin V–positive and propidium iodine–negative/positive (early and late apoptosis, respectively). For determination of cell proliferation, the Lymphoma cell lines are exposed to Pomalidomide (2.5, 5, 10, 20, and 40 μg/mL) for 24 hours or 48 hours. 1 μCi per well (96-well plate) of [3H]-thymidine is added and cells are incubated for another 18 hours. Cells are then harvested using the Harvest system into the 96-well glass filters and [3H]-thymidine uptake is measured using an automated scintillation counter.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: Disseminated lymphoma-bearing SCID mice
  • Formulation: Dissolved in DMSO to make a 10 mg/mL stock solution and diluted to a final concentration of 1 mg/mL in sterile 0.9% normal saline.
  • Dosages: 0.5 mg/kg
  • Administration: Injection i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 54 mg/mL (197.62 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
3mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 273.24
Formula

C13H11N3O4

CAS No. 19171-19-8
Storage powder
in solvent
Synonyms CC-4047

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03732703 Not yet recruiting Relapsed Refractory Multiple Myeloma Multiple Myeloma Research Foundation|AbbVie|Celgene Corporation|Eli Lilly and Company|Genentech Inc.|Janssen LP|Takeda|Multiple Myeloma Research Consortium December 10 2018 Phase 1|Phase 2
NCT03715478 Not yet recruiting Relapsed and/or Refractory Multiple Myeloma Myeloma Canada Research Network|GlaxoSmithKline December 1 2018 Phase 1|Phase 2
NCT03227432 Withdrawn Multiple Myeloma Dana-Farber Cancer Institute|Bristol-Myers Squibb December 2018 Phase 2
NCT03651128 Not yet recruiting Multiple Myeloma Celgene November 30 2018 Phase 3
NCT03713294 Not yet recruiting Refractory Plasma Cell Myeloma Mayo Clinic|National Cancer Institute (NCI) November 8 2018 Phase 2
NCT03520985 Recruiting Refractory Multiple Myeloma Swiss Group for Clinical Cancer Research October 1 2018 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

  • Question 1:

    Is S1567 in the 1% DMSO+30% polyethylene glycol+1% Tween 80 suitable for oral administration?

  • Answer:

    S1567 in 1% DMSO+30% polyethylene glycol+1% Tween 80 is a suspension. This formulation is for oral gavege.

  • Question 2:

    I would like to know if the pomalidomide is racemic or optically active?

  • Answer:

    Our S1567 Pomalidomide is racemic.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID