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Methyclothiazide

Cat.No.S4057

Methyclothiazide is a substituted benzothiadiazide, used to treat high blood pressure and fluid retention caused by various conditions including heart disease.
Methyclothiazide Chemical Structure

Chemical Structure

Molecular Weight: 360.24

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Quality Control

Batch: S405701 DMSO]72 mg/mL]false]Ethanol]4 mg/mL]false]Water]Insoluble]false Purity: 99.84%
99.84

Solubility

In vitro
Batch:

DMSO : 72 mg/mL (199.86 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 4 mg/mL

Water : Insoluble

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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 360.24 Formula

C9H11Cl2N3O4S2

 Storage (From the date of receipt)
CAS No. 135-07-9 -- Storage of Stock Solutions

Synonyms N/A Smiles CN1C(NC2=CC(=C(C=C2S1(=O)=O)S(=O)(=O)N)Cl)CCl

Mechanism of Action

Features
A diuretic-antihypertensive agent, and is a member of the benzothiadiazine (thiazide) class of drugs.
In vitro
Methyclothiazide (0.1 mM) reduces Ca2+ contractures with maximal inhibition of 90.4% in SHR aortic rings with endothelium. The inhibitory effect of this compound on Ca2+ contracture is significantly but not totally abolished by the NO synthase inhibitor in SHR aortic rings with functional endothelium. This chemical induces endothelium-dependent inhibition of the vasoconstrictor responses to NE and AVP only in aortas from spontaneously hypertensive rats (SHR) rather than normotensive Wistar Kyoto rats (WKY), and the maximal vasoconstrictive effect of NE and AVP is decreased by 59% and 32.3%, respectively. It inhibits renin release in rat kidney slices.
In vivo
Methyclothiazide (0.1 g/L for the first 5 weeks and 0.2 g/L for the second 2 weeks, drinking water) attenuates development of hypertension in salt-sensitive (DS) rats, but does not affect blood pressure in salt-resistant (DR) ones. The compound-treated DOCA-salt rats weigh less than controls or the nonsupplemented DOCA-salt rats at weeks 3 and 4. This chemical added to the drinking water of DOCA-salt rats attenuates the elevation of blood pressure.
References
  • [4] https://pubmed.ncbi.nlm.nih.gov/6191135/
  • [5] https://pubmed.ncbi.nlm.nih.gov/6502916/

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