Name: Silibinin (NSC 651520)
Brand: Selleck Chemicals
SKU: S2357
Availability: InStock
Rating: 5 (11 reviews)

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Quality Control

Batch: Purity: 99.93%

99.93

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Solubility

In vitro
Batch:

DMSO : 96 mg/mL (198.98 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.8mg/ml (9.95mM)	Taking the 1 mL working solution as an example, add 50 μL of 96 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.66mg/ml (1.37mM)	Taking the 1 mL working solution as an example, add 50 μL of 13.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	482.44	Formula	C₂₅H₂₂O₁₀	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	22888-70-6	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Silybin, Silibinin A, Silymarin I, Flavobin			SMILES	COC1=C(C=CC(=C1)C2C(OC3=C(O2)C=C(C=C3)C4C(C(=O)C5=C(C=C(C=C5O4)O)O)O)CO)O

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro

Silibinin (NSC 651520) is a mixture of two diastereomers, silibinin A and silybinin B, in approximately equimolar ratio. Both in vitro and animal research suggest that this compound has hepatoprotective (antihepatotoxic) properties that protect liver cells against toxins. It has also demonstrated in vitro anti-cancer effects against human prostate adenocarcinoma cells, estrogen-dependent and -independent human breast carcinoma cells, human ectocervical carcinoma cells, human colon cancer cells, and both small and nonsmall human lung carcinoma cells.

References

[1] https://pubmed.ncbi.nlm.nih.gov/19156713/
[2] https://pubmed.ncbi.nlm.nih.gov/10660092/
[3] https://pubmed.ncbi.nlm.nih.gov/17950073/

[4] https://pubmed.ncbi.nlm.nih.gov/12894553/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2025-12-02)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06964815	RECRUITING	Glioblastoma; IDH Wild-type and STAT3-positive Glioblastoma	Istituto Oncologico Veneto IRCCS	2025-11-12
NCT07561892	RECRUITING	Acute Myeloid Leukemia; Newly Diagnosed; Chemotherapy Effect	Fujian Medical University Union Hospital	2022-04-01	PHASE4
NCT05689619	RECRUITING	Brain Metastases, Adult; Non Small Cell Lung Cancer; Breast Cancer	A.O.U. Città della Salute e della Scienza	2023-09-01
NCT05793489	ACTIVE_NOT_RECRUITING	Brain Metastases, Adult	Istituto Clinico Humanitas	2023-03-06
NCT05497765	COMPLETED	Nonalcoholic Fatty Liver Disease	Guangzhou Medical University	2022-08-01
NCT05069298	UNKNOWN	Overweight and Obesity; Hypercholesterolemia; Hypertriglyceridemia	Universidad Católica San Antonio de Murcia	2023-01-12

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Silibinin (NSC 651520)

Chemical Structure

Molecular Weight: 482.44