NG25

For research use only.

Catalog No.S8868

1 publication

NG25 Chemical Structure

CAS No. 1315355-93-1

NG25 is a novel inhibitor of TAK1 with enzymatic IC50s of 149 and 21.7 nM for TAK1 and MAP4K2 respectively.

Selleck's NG25 has been cited by 1 publication

Purity & Quality Control

Choose Selective TAK1 Inhibitors

Biological Activity

Description NG25 is a novel inhibitor of TAK1 with enzymatic IC50s of 149 and 21.7 nM for TAK1 and MAP4K2 respectively.
Targets
TAK1 [1]
()
MAP4K2 [1]
()
In vitro

NG25 can inhibit phosphorylation of proteins predicted to be downstream of TAK1 and MAP4K2 such as IKK, p38 and JNK at concentrations of less than 100 nM[1].

Assay
Methods Test Index PMID
Western blot
p-p38 / p38 / IκBα ; 

PubMed: 27599572     


Breast cancer cell lines T-47D, MCF7, HCC1954, MDA-MB-231, and BT-549 were treated with Dox (20 μM) alone or combined with NG25 (2 μM) for 0, 2 h, 4 h or 6 h. The protein extracts were subjected to SDS-PAGE and immunoblotted with the antibodies against p-p38, p38, and IκBα. β-actin were detected as loading controls for whole cell extracts.

27599572
Growth inhibition assay
Cell viability ; 

PubMed: 27599572     


(a) Cytotoxic effect of NG25 on breast cancer cells in CCK-8 assay. Five human breast cancer cell lines T-47D, MCF7, HCC1954, MDA-MB-231, and BT-549 were treated with NG25 at 0, 1 μM, 2 μM, 5 μM, 10 μM, and 20 μM for 72 h, then subjected to a CCK-8 assay. The absorbance of each well was measured at 450 nm and plotted for the cell viability curve. The data are represented as mean ± SD. P values < 0.01 (**) or < 0.001 (***) were indicated. (b) IC50 values of NG25 in breast cancer cell lines were listed. 

27599572

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL (186.01 mM)
Ethanol 2 mg/mL (3.72 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 537.58
Formula

C29H30F3N5O2

CAS No. 1315355-93-1
Storage powder
in solvent
Synonyms N/A
Smiles CCN1CCN(CC1)CC2=C(C=C(C=C2)NC(=O)C3=CC(=C(C=C3)C)OC4=C5C=CNC5=NC=C4)C(F)(F)F

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID