Home MAPK TAK1 inhibitor - MAP4K inhibitor NG25 For research use only.

NG25

NG25 is a novel inhibitor of TAK1 with enzymatic IC50s of 149 and 21.7 nM for TAK1 and MAP4K2 respectively.

NG25 Chemical Structure

NG25 Chemical Structure

CAS: 1315355-93-1

Selleck's NG25 has been cited by 3 publications

Purity & Quality Control

Batch: Purity: 99.82%
99.82

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Biological Activity

Description NG25 is a novel inhibitor of TAK1 with enzymatic IC50s of 149 and 21.7 nM for TAK1 and MAP4K2 respectively.
Targets
TAK1 [1] MAP4K2 [1]
In vitro
In vitro

NG25 can inhibit phosphorylation of proteins predicted to be downstream of TAK1 and MAP4K2 such as IKK, p38 and JNK at concentrations of less than 100 nM[1].
Cell Research Cell lines AM-1 cells
Concentrations 100 nM
Incubation Time 1 h
Method

For the inhibition of TAK1 expression, cells were preincubated in DMEM with 100 nM NG25 at 37 °C, 5% CO2 for 1 h.
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-p38 / p38 / IκBα 27599572
Growth inhibition assay Cell viability 27599572

Chemical Information & Solubility

Molecular Weight 537.58 Formula

C29H30F3N5O2
CAS No. 1315355-93-1 SDF --
Smiles CCN1CCN(CC1)CC2=C(C=C(C=C2)NC(=O)C3=CC(=C(C=C3)C)OC4=C5C=CNC5=NC=C4)C(F)(F)F
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 25 mg/mL ( (46.5 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble


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In vivo
Batch:

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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