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EdU (5-Ethynyl-2'-deoxyuridine)

Name: EdU (5-Ethynyl-2'-deoxyuridine)
Brand: Selleck Chemicals
SKU: S1661
Rating: 5 (1 reviews)

Synonyms: 5-EdU, 2'-Deoxy-5-ethynyluridine

Catalog No.S1661 Purity: 99.82%

EdU (5-Ethynyl-2'-deoxyuridine), a thymidine analogue, which can be incorporated into cellular DNA and the subsequent reaction of EdU with a fluorescent azide in a copper-catalyzed [3+2] cycloaddition ("Click" reaction) are developed as a method for detection of DNA synthesis. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that is applicable to the synthesis of PROTACs.

EdU (5-Ethynyl-2'-deoxyuridine) Chemical Structure

CAS: 61135-33-9

Selleck's EdU (5-Ethynyl-2'-deoxyuridine) has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.82%

99.82

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Biological Activity

Description	EdU (5-Ethynyl-2'-deoxyuridine), a thymidine analogue, which can be incorporated into cellular DNA and the subsequent reaction of EdU with a fluorescent azide in a copper-catalyzed [3+2] cycloaddition ("Click" reaction) are developed as a method for detection of DNA synthesis. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that is applicable to the synthesis of PROTACs.

In Vivo
In vivo	5-Ethynyl-2'-deoxyuridine (EdU, 5-EdU, 2'-Deoxy-5-ethynyluridine), a thymidine analogue developed for detection of DNA synthesis, the number of EdU positive cells in the dentate gyrus of the hippocampus (DG) were slightly increased in a dose dependent manner in voluntary exercise (running) mouse groups, while decreased in restraint stress mouse model significantly.^[2]
Animal Research	Animal Models	Voluntary exercise mouse model, Restraint stress mouse model
	Dosages	10, 20, 50, 100 or 200 mg/kg
	Administration	i.p.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05923944	Not yet recruiting	Anxiety\|Anxiety Disorders and Symptoms	School of Health Sciences Geneva\|University of Geneva Switzerland\|University of Haifa\|Swiss National Science Foundation	January 2024	Not Applicable
NCT05599191	Recruiting	Refractory Chronic Cough	Bellus Health Inc. - a GSK company	October 25 2022	Phase 3
NCT05459974	Recruiting	Atrial Fibrillation	Stony Brook University	June 20 2022	Phase 3
NCT05160935	Completed	Coronary Angiography\|Anxiety\|Vital Signs\|Education\|Nursing\|Nursing Care	Eskisehir Osmangazi University	January 1 2022	Not Applicable

References

Chemical Information & Solubility

Molecular Weight	252.22	Formula	C₁₁H₁₂N₂O₅
CAS No.	61135-33-9	SDF	--
Smiles	C#CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)O
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 50 mg/mL ( (198.23 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 10 mg/mL

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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