CAS No. 61135-33-9

Batch: Purity:99.82
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5-Ethynyl-2'-deoxyuridine (EdU) Chemical Structure
Chemical Structure
Check the 5-Ethynyl-2'-deoxyuridine (EdU) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 5-ethynyl-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione
CAS Number 61135-33-9
Molecular Formula

C11H12N2O5
Molecular Weight (MW) 252.22
SMILES C#CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)O
InChIKey CDEURGJCGCHYFH-DJLDLDEBSA-N

Ordering Information

Biological Activity

5-Ethynyl-2'-deoxyuridine (EdU) is a thymidine analogue that acts as a probe for measuring cellular DNA synthesis and cell proliferation. Upon incorporation into newly synthesized DNA during S-phase, EdU reacts with fluorescent azides via copper-catalyzed click chemistry ([3+2] cycloaddition), enabling rapid and sensitive detection of replicating cells without requiring DNA denaturation. Additionally, EdU serves as an alkyl chain-based linker in the synthesis of proteolysis targeting chimeras (PROTACs). [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 50 mg/mL (198.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 2 mg/mL
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.