Sal003 Chemical Structure

Sal003 Chemical Structure
Molecular Weight: 463.21

Validation & Quality Control

Quality Control & MSDS

Product Information

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Product Description

Biological Activity

Description Sal003 is a potent and cell-permeable eIF-2α phosphatase inhibitor.
Targets eIF-2α phosphatase [1]
In vitro Sal003 increases eIF2α phosphorylation status by blocking eIF2a phosphatases in cells. [1] In mouse embryonic fibroblasts (MEFs), Sal003 causes dissociation of polysomes by increasing eIF2a phosphorylation. Sal003 impairs late-long-term potentiation (L-LTP) in an ATF4-dependent mode in hippocampal slices from WT mice. [2] In addition, eIF2α phosphorylation by Sal003 also enhances apoptotic signaling induced by subtilase cytotoxin (SubAB). [3]
In vivo Sal003 impairs spatial long-term memory formation in rats. [2]
Features

Protocol(Only for Reference)

Animal Study: [2]

Animal Models Rats (300–325g body weight).
Formulation Dissolved in DMSO and further diluted in 0.9% NaCl to a final DMSO concentration of 0.1%.
Dosages 2 μL of 20 μM
Administration Intrahippocampal Injection

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)0.020.151.80.40.0810
Body Surface Area (m2)0.0070.0250.150.050.020.5
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

References

[1] Robert F, et al. Mol Biol Cell. 2006, 17(11), 4632-4644.

[2] Costa-Mattioli M, et al. Cell. 2007, 129(1), 195-206.

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Chemical Information

Molecular Weight (MW) 463.21
Formula

C18H15Cl4N3OS

CAS No. 1164470-53-4
Storage 3 years -20℃powder
2 years -80℃in solvent
Synonyms N/A
Solubility (25°C) * In vitro DMSO 93 mg/mL (200.77 mM)
Water <1 mg/mL
Ethanol <1 mg/mL
In vivo
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name 3--Phenyl-N-(2,2,2-trichloro-1-((((4-chlorophenyl)amino)carbonothioyl)amino)ethyl)acrylamide

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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