Home Hippo LATS inhibitor - YAP inhibitor TRULI For research use only.

TRULI

TRULI inhibits both Lats1 and Lats2 with a IC50 of 0.2 nM, suppresses Yap phosphorylation, induces cell proliferation in several cell lines and tissues, and promotes the initial stages of proliferative regeneration of the sensory receptors in the inner ear.

TRULI Chemical Structure

TRULI Chemical Structure

CAS: 1424635-83-5

Purity & Quality Control

Batch: E106101 DMSO] 67 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.98%
99.98

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Biological Activity

Description TRULI inhibits both Lats1 and Lats2 with a IC50 of 0.2 nM, suppresses Yap phosphorylation, induces cell proliferation in several cell lines and tissues, and promotes the initial stages of proliferative regeneration of the sensory receptors in the inner ear.
Targets
Lats2 [1]
(in the Vitro kinase assay)		 Lats1 [1]
(in vitro kinase assay)		 Lats2 [1]
(in vitro kinase assay)
0.2 nM 0.2 nM 0.2 nM
In vitro
In vitro

TRULI acts as an inhibitor of Lats kinases in vitro, suppresses Yap phosphorylation, induces cell proliferation in several cell lines and tissues, and promotes the initial stages of proliferative regeneration of the sensory receptors in the inner ear.[1]
Cell Research Cell lines MCF 10A cells, HEK293A cells
Concentrations 10 μM
Incubation Time 24 h, 72 h, 5 days
Method

For the small-molecule screen, chemical-library plates are thawed at room temperature and 0.1 μL of TRULI is placed in a well of a 384-well assay plate containing 10 μL of MCF 10A culture medium. MCF 10A cells are then plated in 40 μL of MCF 10A culture medium to achieve a final concentration of 10 μM for TRULI and 0.25% DMSO. After 24 h incubation, the fraction of the cells with nuclear Yap are determined and compared that value to the median negative-control value. For quantifying the proliferation of supporting cells, utricles are imaged at ×60, and then assembled into a composite tiling.

Chemical Information & Solubility

Molecular Weight 334.40 Formula

C18H14N4OS
CAS No. 1424635-83-5 SDF --
Smiles O=C(N=C1SC=CN1CC2=CC=CC=C2)C3=C[NH]C4=NC=CC=C34
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 67 mg/mL ( (200.35 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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