Home Hippo YAP inhibitor - TEAD inhibitor TED-347 For research use only.

TED-347

TED-347 is a potent, irreversible, covalent and allosteric inhibitor of the TEAD⋅Yap protein-protein interaction. TED-347 inhibits TEAD4⋅Yap1 protein-protein interaction with an apparent EC50 of 5.9 μM.

TED-347 Chemical Structure

TED-347 Chemical Structure

CAS: 2378626-29-8

Selleck's TED-347 has been cited by 4 publications

Purity & Quality Control

Batch: S895101 DMSO] 69 mg/mL] false] Ethanol] 25 mg/mL] false] Water] ˂1 mg/mL] false Purity: 99.72%
99.72

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Biological Activity

Description TED-347 is a potent, irreversible, covalent and allosteric inhibitor of the TEAD⋅Yap protein-protein interaction. TED-347 inhibits TEAD4⋅Yap1 protein-protein interaction with an apparent EC50 of 5.9 μM.
Targets
TEAD4⋅Yap1 [1]
(Cell-free assay)
5.9 μM(EC50)
In vitro
In vitro

TED-347 is found to inhibit the protein-protein interaction in a time-dependent manner. TED-347 is shown to functionally disrupt the TEAD·Yap1 interaction in cells and to reduce the viability of patient-derived glioblastoma cell lines.[1]
Cell Research Cell lines HEK-293 cells, GBM43 cells
Concentrations 0.5 µM, 1.0 µM, 5.0 µM, 10 µM, 25 µM
Incubation Time 48 h
Method

HEK-293 or GBM43 cells are plated at 2.43104 cells/well in a 96-well microplate and are transfected after 24 hr with the a pGL3.1 reporter containing the CTGF promoter and a plasmid encoding TK-Renilla luciferase in combination with control vectors or vectors that express Yap1 and TEAD4. After 48 hr, cells are treated with 0.5, 1.0, 5.0 or 10 µM of TED-347 for another 48 hr.

Chemical Information & Solubility

Molecular Weight 313.70 Formula

C15H11ClF3NO
CAS No. 2378626-29-8 SDF --
Smiles C1=CC=C(C(=C1)C(=O)CCl)NC2=CC=CC(=C2)C(F)(F)F
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 69 mg/mL ( (219.95 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 25 mg/mL

Water : ˂1 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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