TED-347

For research use only.

Catalog No.S8951

TED-347 Chemical Structure

CAS No. 2378626-29-8

TED-347 is a potent, irreversible, covalent and allosteric inhibitor of the TEAD⋅Yap protein-protein interaction. TED-347 inhibits TEAD4⋅Yap1 protein-protein interaction with an apparent EC50 of 5.9 μM.

Purity & Quality Control

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Biological Activity

Description TED-347 is a potent, irreversible, covalent and allosteric inhibitor of the TEAD⋅Yap protein-protein interaction. TED-347 inhibits TEAD4⋅Yap1 protein-protein interaction with an apparent EC50 of 5.9 μM.
Targets
TEAD4⋅Yap1 [1]
(Cell-free assay)
5.9 μM(EC50)
In vitro

TED-347 is found to inhibit the protein-protein interaction in a time-dependent manner. TED-347 is shown to functionally disrupt the TEAD·Yap1 interaction in cells and to reduce the viability of patient-derived glioblastoma cell lines.[1]

Protocol

Cell Research:

[1]

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  • Cell lines: HEK-293 cells, GBM43 cells
  • Concentrations: 0.5 µM, 1.0 µM, 5.0 µM, 10 µM, 25 µM
  • Incubation Time: 48 h
  • Method:

    HEK-293 or GBM43 cells are plated at 2.43104 cells/well in a 96-well microplate and are transfected after 24 hr with the a pGL3.1 reporter containing the CTGF promoter and a plasmid encoding TK-Renilla luciferase in combination with control vectors or vectors that express Yap1 and TEAD4. After 48 hr, cells are treated with 0.5, 1.0, 5.0 or 10 µM of TED-347 for another 48 hr.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 69 mg/mL (219.95 mM)
Water ˂1 mg/mL
Ethanol '25 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 313.70
Formula

C15H11ClF3NO

CAS No. 2378626-29-8
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C(C(=C1)C(=O)CCl)NC2=CC=CC(=C2)C(F)(F)F

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID