Cell-free Assays (IC50/Ki) for BIBF 1120 (Nintedanib)

Key TargetIC50 (Cell-free)DescriptionReference
Human recombinant VEGFR2 7 nM TKI activity assay Download PDF
VEGFR2 7 nM Radiometric detection method Download PDF
VEGFR2 7 nM enzymatic radiometric assay (using [γ-33P]-ATP) PMC9161413
VEGFR, FGFR, PDGFR 13–34 nM (VEGF), 37–108 nM (FGF), 59–65 nM (PDGF) Biochemical assay (implied) Download PDF
PDGF receptor (R)-α 59 nmol/L Kinase inhibition assay PMC3693725

Cell-based Assay (IC50) for BIBF 1120 (Nintedanib)

Cell TypeIC50 (Cell-based)DescriptionReference
RPE cells 18.8 mM (NTB-PBS), 29.5 mM (NTB-LIP) MTT method PMC12085219
IPF FBs 4.16 µM MTT assay Download PDF
HPFs 2.38 µM MTT assay Download PDF
AGS cells 5.3 ± 0.9 mM CCK-8 assay Download PDF
MKN28 cells 6.1 ± 1.2 mM CCK-8 assay Download PDF
Check the BIBF 1120 (Nintedanib) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

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