MYF-01-37

Catalog No.S8950

For research use only.

MYF-01-37 is a novel covalent inhibitor of TEAD targeting Cys380 when incubated with the TEAD2 protein.

MYF-01-37 Chemical Structure

CAS No. 2416417-65-5

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Biological Activity

Description MYF-01-37 is a novel covalent inhibitor of TEAD targeting Cys380 when incubated with the TEAD2 protein.
Targets
TEAD [1]
()
In vitro

MYF-01-37 is developed as a covalent binder to TEAD, targeting Cys380 when incubated with the TEAD2 protein (C359 in TEAD1). Pretreating cells with MYF-01-37 leads to loss of direct TEAD pull-down by biotin-MYF-01-037 from whole-cell lysate, confirming MYF-01-037 binding to TEAD in cells.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: PC9 cells
  • Concentrations: 10 μM, 1 μM
  • Incubation Time: 6 h
  • Method:

    Cells are treated with test compounds for 6 h at the indicated doses. Total cell lysates are prepared using pulldown buffer. To pull down TEADs, 1 mg of total protein is combined with biotinylated MYF-01-37 at 1 μM and rotated at 4 ℃ for 6 hrs followed by RT for 1 h. Subsequently, streptavidin agarose resin (30 ml 50% slurry) is added and samples rotated for another 2 h at 4 ℃. The resin is subsequently washed 3× with pulldown buffer, and TEADs released from the resin by boiling for 10 min in 2× gel loading buffer and resolved by western blotting. As a loading control, 25 mg of total protein is used.

Solubility (25°C)

In vitro

DMSO 60 mg/mL
(201.13 mM)
Water Insoluble
Ethanol ''60 mg/mL

Chemical Information

Molecular Weight 298.3
Formula

C15H17F3N2O

CAS No. 2416417-65-5
Storage 3 years -20°C powder
2 years -80°C in solvent

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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