CA3(CIL56)

Catalog No.S8661

CA3(CIL56) Chemical Structure

Molecular Weight(MW): 489.61

CA3 has potent inhibitory effects on YAP1/Tead transcriptional activity and primarily targets YAP1 high and therapy-resistant esophageal adenocarcinoma cells endowed with CSC properties.

Size Price Stock Quantity  
USD 147 In stock
USD 447 In stock
Bulk Discount
Bulk Inquiry

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 1 publication

Purity & Quality Control

Choose Selective Hippo pathway Inhibitors

Biological Activity

Description CA3 has potent inhibitory effects on YAP1/Tead transcriptional activity and primarily targets YAP1 high and therapy-resistant esophageal adenocarcinoma cells endowed with CSC properties.
Targets
YAP/TEAD interaction [1]
()
In vitro

CA3 strongly inhibits esophageal adenocarcinoma cell growth in vitro. CA3 can effectively suppress tumor cell proliferation, induce apoptosis, reduce tumor sphere formation, and the population of ALDH1+ cells. CA3 specially inhibits Tead/YAP1 transcriptional activity but shows no inhibitory activity on other transcriptional factors-Super-TOP/Wnt, CBF1/Notch, and AP-1 after cotransfection of their respective individual promoter luciferases in 293T cells. CA3 preferentially inhibits CSC properties enriched in radiation-resistant esophageal adenocarcinoma cells[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT-29 cells NYW3SWllTnWwY4Tpc44h[XO|YYm= NWX3[Wx6UW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCrbjDoeY1idiCKVD2yPUBk\WyuczDhd5Nme3OnZDDhd{Bl\XCuZYTpc44hd2ZiYnX0ZU1k[XSnbnnuJIxmfmWuIHL5JHRETiCudXPp[oVz[XOnIIDyc41wfGW{IILldI9zfGW{IHHzd4F697zOIFnDOVA:OC57OTFOwG0> MWOyOlE5OjJ|OB?=

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
YAP1 / SOX9 ; 

PubMed: 29167315     


Cell lysate from SKGT-4 and JHESO EAC cells treated with CA3 at indicated dosage were selected for immunoblotting analysis for YAP1 and SOX9.

29167315
Immunofluorescence
YAP1 / SOX9 ; 

PubMed: 29167315     


Immunofluorescent staining of YAP1 and SOX9 in JHESO cells were observed by confocal microscopy (right). 

29167315
Growth inhibition assay
Cell viability; 

PubMed: 29167315     


Four EAC cell lines A.SKGT-4, Flo-1,JHESO and OACP EAC cell lines were treated with 0.1% DMSO (as control), CA3 (left) at dosage of 0.1, 0.5 and 1µM, the cells proliferation and viability were measured using MTS assay and calculated as percent of control. B. SKGT-4 (PINYAP120) cells with (DOX+) or without (DOX−) YAP1 induction were treated with 0.1% DMSO (as control), CA3 (left) at dosage of 0.1, 0.5 and 1µM, the cells proliferation and viability were measured using MTS assay and calculated as percent of control. For all experiments, values shown represent the mean and SD of at least triplicate assays.**P<0.01.

29167315
In vivo CA3 exerts strong antitumor activity in xenograft model with no apparent toxicity[1].

Protocol

Cell Research:

[1]
- Collapse
  • Cell lines: SKGT-4 and JHESO cells
  • Concentrations: 0.5 and 1 μmol/L
  • Incubation Time: 48 hours
  • Method:

    SKGT-4 and JHESO cells are seeded onto 6-well plates (1 × 105/well) in DMEM and cultured for 24 hours to allow for cell attachment. The cells are then treated with 0.1% DMSO (control) or CA3 at different doses as indicated for 48 hours. Next, the cells are harvested, fixed with methanol, washed, treated with RNase A, and stained for DNA with propidium iodide, and their DNA histograms and cell-cycle phase distributions are analyzed using flow cytometry.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 97 mg/mL (198.11 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 489.61
Formula

C23H27N3O5S2
CAS No. 300802-28-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
selleck catalog
Tags: buy CA3(CIL56) | CA3(CIL56) supplier | purchase CA3(CIL56) | CA3(CIL56) cost | CA3(CIL56) manufacturer | order CA3(CIL56) | CA3(CIL56) distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID