Home Biosimilar Antibodies Cytoskeletal Signaling Integrin Vedolizumab (anti-α4β7-integrin)
For research use only. Not for use in humans.

Vedolizumab (anti-α4β7-integrin)

Synonyms: Entyvio

Vedolizumab (anti-α4β7-integrin) is a humanized IgG1 monoclonal antibody that targets the α4β7 integrin for the treatment of ulcerative colitis and Crohn's disease.

Vedolizumab (anti-α4β7-integrin)

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Quality Control

Batch: A204502 Purity: 98% Protein concentration: 5.72mg/ml Endotoxin Level: ＜1EU/mg
98

Specificity

Name Citation Integrin ILK Others
Cilengitide trifluoroacetate 70
A-205804 6
GLPG0187 1
GSK-3008348 HCl 0
Bexotegrast (PLN-74809) 0
Cilengitide (EMD 121974) 36
OSU-T315 1
TR-14035 0
Cyclo(RGDyK) TFA 14
SB273005 27
RGD peptide (Arg-Gly-Asp) 26
ILK-IN-3 0
RGD peptide (GRGDNP) 1 caspase-3
ATN-161 22
Cyclo(-RGDfK) TFA 13
Expand to Check More
1. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation. 2. "✔" indicates inhibitory effect, but without specific value.

Mechanism of Action

Description
Vedolizumab (anti-α4β7-integrin) is a humanized IgG1 monoclonal antibody that targets the α4β7 integrin for the treatment of ulcerative colitis and Crohn's disease.
In vitro

Vedolizumab (anti-α4β7-integrin) binds to a subset of human peripheral blood memory CD4+ T lymphocytes including gut-homing interleukin 17 T-helper lymphocytes at the highest level, also binds to eosinophils at high levels, and to naive T-helper lymphocytes, naive and memory cytotoxic T lymphocytes, B lymphocytes, natural killer cells, and basophils at lower levels; Vedolizumab binds to memory CD4+ T and B lymphocytes with subnanomolar potency with EC50 of 0.3-0.4 nM.
Cell Research

PBMCs were resuspended in assay buffer for CD4 memory cell interleukin (IL) 17A intracellular staining. PBMCs were stimulated (or not stimulated, for the control samples) with 50 ng/ml phorbol 12-myristate 13-acetate and 1 µg/ml Ionomycin in the presence of 3 µM Monensin for 5 h at 37°C. After washing, cells were stained with fluorescent-labeled antibodies: vedolizumab, anti-α4 and anti-β7, anti-CD4, and anti-CD45RO, where applicable, at 4°C for 30 min. Cells were then washed with cold PBS and resuspended in fixation and permeabilization working solution staining buffer at 4°C for 30 min. After fixation and permeabilization, cells were washed with permeabilization buffer and blocked with 10% normal rat serum at 4°C for 10 min. After the blocking step, cells were stained with fluorochrome-conjugated anti_x0002_IL17A antibody at 4°C for 30 min. Finally, cells were washed with permeabilization buffer and analyzed.
References

Product Details

CAS No. 943609-66-3
Molecular Weight 145.7 KD
Isotype Human IgG1
Application Vedolizumab (anti-α4β7-integrin)
Sterility 0.2 μM filtered
Formulation PBS Buffer, PH 7.4
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles.

Comparison of Clones for

*Literature analysis of various clone (for this target) products available on the market shows that Selleck's selected clones are more frequently applied. (data until September 2024)

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