Vedolizumab (anti-α4β7-integrin)

Catalog No.A2045 Batch: A204502

Technical Data

CAS No.	943609-66-3
Formulation	PBS Buffer, PH 7.4
Isotype	Human IgG1
Application	Vedolizumab (anti-α4β7-integrin)
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles.
Purity	98%
Protein concentration	5.72mg/ml
Endotoxin Level	＜1EU/mg

Biological Activity

Description	Vedolizumab (anti-α4β7-integrin) is a humanized IgG1 monoclonal antibody that targets the α4β7 integrin for the treatment of ulcerative colitis and Crohn's disease.
In vitro	Vedolizumab (anti-α4β7-integrin) binds to a subset of human peripheral blood memory CD4⁺ T lymphocytes including gut-homing interleukin 17 T-helper lymphocytes at the highest level, also binds to eosinophils at high levels, and to naive T-helper lymphocytes, naive and memory cytotoxic T lymphocytes, B lymphocytes, natural killer cells, and basophils at lower levels; Vedolizumab binds to memory CD4⁺ T and B lymphocytes with subnanomolar potency with EC50 of 0.3-0.4 nM.^[1]

Description

Vedolizumab (anti-α4β7-integrin) is a humanized IgG1 monoclonal antibody that targets the α4β7 integrin for the treatment of ulcerative colitis and Crohn's disease.

In vitro

Vedolizumab (anti-α4β7-integrin) binds to a subset of human peripheral blood memory CD4⁺ T lymphocytes including gut-homing interleukin 17 T-helper lymphocytes at the highest level, also binds to eosinophils at high levels, and to naive T-helper lymphocytes, naive and memory cytotoxic T lymphocytes, B lymphocytes, natural killer cells, and basophils at lower levels; Vedolizumab binds to memory CD4⁺ T and B lymphocytes with subnanomolar potency with EC50 of 0.3-0.4 nM.^[1]

Protocol (Only for Reference)

Cell Assay:	PBMCs were resuspended in assay buffer for CD4 memory cell interleukin (IL) 17A intracellular staining. PBMCs were stimulated (or not stimulated, for the control samples) with 50 ng/ml phorbol 12-myristate 13-acetate and 1 µg/ml Ionomycin in the presence of 3 µM Monensin for 5 h at 37°C. After washing, cells were stained with fluorescent-labeled antibodies: vedolizumab, anti-α4 and anti-β7, anti-CD4, and anti-CD45RO, where applicable, at 4°C for 30 min. Cells were then washed with cold PBS and resuspended in fixation and permeabilization working solution staining buffer at 4°C for 30 min. After fixation and permeabilization, cells were washed with permeabilization buffer and blocked with 10% normal rat serum at 4°C for 10 min. After the blocking step, cells were stained with fluorochrome-conjugated anti_x0002_IL17A antibody at 4°C for 30 min. Finally, cells were washed with permeabilization buffer and analyzed.

Cell Assay:

PBMCs were resuspended in assay buffer for CD4 memory cell interleukin (IL) 17A intracellular staining. PBMCs were stimulated (or not stimulated, for the control samples) with 50 ng/ml phorbol 12-myristate 13-acetate and 1 µg/ml Ionomycin in the presence of 3 µM Monensin for 5 h at 37°C. After washing, cells were stained with fluorescent-labeled antibodies: vedolizumab, anti-α4 and anti-β7, anti-CD4, and anti-CD45RO, where applicable, at 4°C for 30 min. Cells were then washed with cold PBS and resuspended in fixation and permeabilization working solution staining buffer at 4°C for 30 min. After fixation and permeabilization, cells were washed with permeabilization buffer and blocked with 10% normal rat serum at 4°C for 10 min. After the blocking step, cells were stained with fluorochrome-conjugated anti_x0002_IL17A antibody at 4°C for 30 min. Finally, cells were washed with permeabilization buffer and analyzed.

References	[1] Soler D, et al. J Pharmacol Exp Ther. 2009 Sep;330(3):864-75.

References

[1] Soler D, et al. J Pharmacol Exp Ther. 2009 Sep;330(3):864-75.

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

Specific storage and handling information for each product is indicated on the product datasheet. Most Selleck products are stable under the recommended conditions. Products are sometimes shipped at a temperature that differs from the recommended storage temperature. Short-term storage of many products are stable in the short-term at temperatures that differ from that required for long-term storage.
We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt of the product, follow the storage recommendations on the product data sheet.