Target Selectivity of Quercetin (Sophoretin)
| Key Target | IC50 | Description | Reference |
|---|---|---|---|
| DPP4 | 0.01 μM | GA-QSAR modeling | PMC13084071 |
| CD39 (commercial enzyme) | 0.57 μM | Colorimetric phosphate quantification method (Biomol Green Reagent) | PMC8614122 |
| HER2 | 570.07 nM | HTRF kinEASE TK kit, fluorescent signals correlated with ATP, nonlinear regression with normalized dose-response fit. | PMC8088928 |
| Cell-free system | 0.6 µM | Isoluminol-dependent chemiluminescence (ICL) assay. | PMC10606308 |
| Pim-1 | 1.31 µM | Luminescence-based kinase assay (Pim-1 assay) for IC50, correlated with Steered Molecular Dynamics (SMD) pulling force. | PMC11491346 |
| CD73 (commercial enzyme) | 1.7 μM | Colorimetric phosphate quantification method (Biomol Green Reagent) | PMC8614122 |
| Xanthine Oxidase (commercial enzyme) | 3.0 μM | Continuous spectrophotometric rate determination assay | PMC8614122 |
| ACE2 | 4.48 µM | recombinant human ACE2 activity assay | PMC11944354 |
| sBACE1 | 4.89 μM | β-secretase (BACE1) activity detection kit (FRET method) | PMC12771191 |
| Xanthine Oxidase | 6.45 µM | XO inhibitory activity was calculated by using the linear regression equation in graphpad prism. | PMC9531272 |
| recombinant catalytic subunit of PI3K (human recombinant PI3K-γ (His tagged)) | 14 µM | In vitro kinase assay using a commercially available kit ([Vendor]) by measuring the amount of radioactively labeled [γ-32P] ATP incorporated into a lipid substrate, following separation using thin layer chromatography (TLC). | PMC5522089 |
| Recombinant CYP3A4.1 | 17.59 µM | LC-MS/MS analysis of ziprasidone sulfoxide formation, Michaelis-Menten kinetics, Lineweaver-Burk plot | PMC12069442 |
| Mpro SARS-CoV-1 | 19180 nM | DeepScreeing server using Recurrent Neural Networks (RNN) computing system to develop an inhibitory predictive model using SARS coronavirus Mpro dataset, with PubChem fingerprint for molecular fingerprints. | PMC10008098 |
| HUVEC extracts | 20.0 μM | Continuous spectrophotometric rate determination assay | PMC8614122 |
| SARS-CoV-2 Mpro | 21 µM | Förster resonance energy transfer (FRET) assay, nonlinear regression analysis | PMC8268238 |
| Purine Nucleoside Phosphorylase (commercial enzyme) | 36.9 μM | Continuous spectrophotometric rate determination procedure | PMC8614122 |
| soybean 15-lipoxygenase | 12.35 µg/mL | soybean 15-lipoxygenase (15-LOX) inhibitory assay in the presence of linoleic acid based on the formation of the complex Fe3+/xylenol orange with absorption at 560 nm. | PMC8352634 |
| Recombinant CYP3A4.33 | 54.51 µM | LC-MS/MS analysis of ziprasidone sulfoxide formation | PMC12069442 |
| Adenosine Deaminase (commercial enzyme) | 55.6 μM | Continuous spectrophotometric rate determination assay | PMC8614122 |
| HIV | 58.8 µM | Protease inhibition assay | PMC7318306 |
| CaMKIV | 61.3 μM | BIOMOL® Green reagent-based microtitre plate assay, measuring inorganic phosphate released from ATP. | PMC7382742 |
| hPTP1B 1-400 | 87.1 μM | spectrophotometric and colorimetric method to detect inhibitory activity, followed by non-linear regression using Origin 8.0 software. | PMC10644722 |
| ACE2 | 104.79 µM | ACE2 inhibitory assay | PMC9392260 |
| Na+/K+-ATPase | 1000 µM | Baginsky assay, ouabain-sensitive ATPase activity measurement, data fitted to logistic function | PMC4812144 |
| AChE | 1.22 mM | microtiter assay | PMC12537693 |
| BChE | 2.51 mM | microtiter assay | PMC12537693 |
| Soybean 15-LOX | 3.34 mM | [Vendor]’s lipoxygenase inhibitor screening assay kit | PMC7170299 |
| SARS-CoV 3CLpro | 52.7 mM | FRET | PMC7833040 |
| PD-1/PD-L1 interaction | 29.93 mg/ml | ELISA assay | PMC11328830 |
| Chemical radical scavenging system | 172.13 mM | DPPH assay | PMC10064984 |
| PTP1B | 0.38 μg/ml | GA-QSAR modeling | PMC13084071 |
| Candida parapsilosis | 0.5 μg/ml | MIC determination, CLSM analysis | PMC11594109 |
| Soybean 15-LOX | 18.72 μg/ml | Soybean 15-LOX inhibitory assay (FOX complex formation, absorbance at 560 nm) | PMC10320002 |
| 15-LOX | 61.82 μg/ml | 15-LOX inhibition assay, measuring absorbance at 234 nm. | PMC7591100 |
Check the
Quercetin (Sophoretin)
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Cell-free Assays (IC50/Ki) for Quercetin (Sophoretin)
| Key Target | IC50 (Cell-free) | Description | Reference |
|---|---|---|---|
| HIV protease | 58.8 µM | Protease inhibition assay | Download PDF |
| Mpro of SARS-CoV-1 | 19180 nM | DeepScreeing server using Recurrent Neural Networks (RNN) computing system to develop an inhibitory predictive model using SARS coronavirus Mpro dataset, with PubChem fingerprint for molecular fingerprints. | PMC10008098 |
| 15-LOX enzyme | 61.82±5.5 µg/mL | 15-LOX inhibition assay, measuring absorbance at 234 nm. | PMC7591100 |
| CaMKIV | 61.3 μM | BIOMOL® Green reagent-based microtitre plate assay, measuring inorganic phosphate released from ATP. | Download PDF |
| HER2 kinase | 570.07 nmol/L | HTRF kinEASE TK kit, fluorescent signals correlated with ATP, nonlinear regression with normalized dose-response fit. | PMC8088928 |
| Adenosine Deaminase (commercial enzyme) | 55.6 µM | Continuous spectrophotometric rate determination assay | Download PDF |
| HUVEC protein extracts | 20.0 µM | Continuous spectrophotometric rate determination assay | Download PDF |
| Xanthine Oxidase (commercial enzyme) | 3.0 µM | Continuous spectrophotometric rate determination assay | Download PDF |
| Purine Nucleoside Phosphorylase (commercial enzyme) | 36.9 µM | Continuous spectrophotometric rate determination procedure | Download PDF |
| CD39 (commercial enzyme) | 0.57 μM | Colorimetric phosphate quantification method (Biomol Green Reagent) | Download PDF |
| CD73 (commercial enzyme) | 1.7 μM | Colorimetric phosphate quantification method (Biomol Green Reagent) | Download PDF |
| SARS-CoV-2 Mpro | 21 µM | Förster resonance energy transfer (FRET) assay, nonlinear regression analysis | Download PDF |
| recombinant human ACE2 | 4.48 µM | recombinant human ACE2 activity assay | Download PDF |
| Soybean 15-LOX enzyme | 3.34mM | [Vendor]’s lipoxygenase inhibitor screening assay kit | Download PDF |
| recombinant catalytic subunit of PI3K (human recombinant PI3K-γ (His tagged)) | 14 µM | In vitro kinase assay using a commercially available kit ([Vendor]) by measuring the amount of radioactively labeled [γ-32P] ATP incorporated into a lipid substrate, following separation using thin layer chromatography (TLC). | Download PDF |
| Xanthine Oxidase | 6.45 ±0.26 µM | XO inhibitory activity was calculated by using the linear regression equation in graphpad prism. | Download PDF |
| AChE | 1.22 ± 0.79 mM | microtiter assay | Download PDF |
| BChE | 2.51 ± 0.04 mM | microtiter assay | Download PDF |
| Candida parapsilosis complex | 0.5 - 16 µg/mL | MIC determination, CLSM analysis | Download PDF |
| Recombinant human CYP3A4.1 | 17.59 ± 1.01 μM | LC-MS/MS analysis of ziprasidone sulfoxide formation, Michaelis-Menten kinetics, Lineweaver-Burk plot | Download PDF |
| Recombinant human CYP3A4.33 | 54.51 ± 1.35 μM | LC-MS/MS analysis of ziprasidone sulfoxide formation | Download PDF |
| Pim-1 kinase | 1.31 µM | Luminescence-based kinase assay (Pim-1 assay) for IC50, correlated with Steered Molecular Dynamics (SMD) pulling force. | Download PDF |
| soybean 15-lipoxygenase | 12.35 µg/mL | soybean 15-lipoxygenase (15-LOX) inhibitory assay in the presence of linoleic acid based on the formation of the complex Fe3+/xylenol orange with absorption at 560 nm. | PMC8352634 |
| ACE2 | 104.79 ± 8.26 μM | ACE2 inhibitory assay | PMC9392260 |
| hPTP1B 1-400 | 87.1 μM | spectrophotometric and colorimetric method to detect inhibitory activity, followed by non-linear regression using Origin 8.0 software. | Download PDF |
| Soybean 15-LOX | 18.72 µg/mL (61.94 µM) | Soybean 15-LOX inhibitory assay (FOX complex formation, absorbance at 560 nm) | Download PDF |
| sBACE1 | 4.89 μM | β-secretase (BACE1) activity detection kit (FRET method) | PMC12771191 |
| SARS-CoV 3CLpro | 52.7 mM | FRET | PMC7833040 |
| Na+/K+-ATPase | >1000 µM | Baginsky assay, ouabain-sensitive ATPase activity measurement, data fitted to logistic function | Download PDF |
| PTP1B | 0.38 μg/ml | GA-QSAR modeling | Download PDF |
| DPP4 | 0.01 μM | GA-QSAR modeling | Download PDF |
Cell-based Assay (IC50) for Quercetin (Sophoretin)
| Cell Type | IC50 (Cell-based) | Description | Reference |
|---|---|---|---|
| Vero cells | 35.7 µg/mL | Inhibition of DENV-2 replication, DENV-2 RNA reduction | Download PDF |
| GES-1, AGS, HGC27, NCI-N87 | GES-1: 327.31 µmol/L, AGS: 41.61 µmol/L, HGC27: 26.76 µmol/L | CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) Kit | PMC11479523 |
| AGS (control), HGC27 (control), NCI-N87 (control), AGS (NUF2 depleted), HGC27 (NUF2 depleted), NCI-N87 (NUF2 depleted) | Control AGS: 42.82 µmol/L, Control HGC27: 29.51 µmol/L, Cont | CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) Kit | PMC11479523 |
| MKN-45 cells | 19.27 μmol/L | CCK-8 assay, absorbance measured at 450 nm, cell viability expressed as percentage of control values. | PMC8088928 |
| MCF-10AT | 52.39 µM | MTT method, absorbance at 490 nm ([Vendor]) | Download PDF |
| MCF-7 | 53.76 µM | MTT method, absorbance at 490 nm ([Vendor]) | Download PDF |
| MDA-MB-231 | 64.23 μM | MTT method, absorbance at 490 nm ([Vendor]) | Download PDF |
| Vero cells (SARS-CoV-2 replication) | 192 µM | RT-qPCR analysis of SARS-CoV-2 RNA copies | Download PDF |
| MDCK cells | 16.96 μM | CCK-8 assay | Download PDF |
| HepG2, MCF-7 | 37 mM | Cell viability assay | Download PDF |
| Micrococcus flavus | 0.125 µg/mL | Microdilution assay | Download PDF |
| TRPC5-expressing (Tet+) HEK 293 cells | 6.5 μM | Intracellular Ca2+ measurement using XRhod-1, concentration-response data fitted with Hill equation | Download PDF |
| A431 human skin cancer cell lines | 579.05 µM | MTT assay | Download PDF |
| J82 | 29.69 μM | CCK8 (Cell Counting Kit-8) assay | Download PDF |
| EBY.S7 cells expressing GLUT2 ΔloopS_Q455R | 3.22 ± 0.45 µM | Transport activity assay using C14-glucose, followed by filtration, scintillation counting, and nonlinear fit analysis with GraphPad Prism. | Download PDF |
| EBY.S7 cells expressing GLUT3 S66Y | 11.6 ± 0.6 µM | Transport activity assay using C14-glucose, followed by filtration, scintillation counting, and nonlinear fit analysis with GraphPad Prism. | Download PDF |
| SCC47 | 27.29 µM (24h), 37.43 µM (48h) | Crystal violet assay | Download PDF |
| FaDu | 33.15 µM (24h), 34.26 µM (48h) | Crystal violet assay | Download PDF |
| human platelet-rich plasma | 520.88 µM | Light Transmittance Aggregometry | Download PDF |
| A2058 | >100 µM | resazurin (Alamar Blue) assay; fitting a sigmoid curve on the data points using Microcal ™Origin2021 software | Download PDF |
| HT-29 | >100 µM | resazurin (Alamar Blue) assay; fitting a sigmoid curve on the data points using Microcal ™Origin2021 software | Download PDF |
| Vero E6 | 15.9 ± 0.7 µM | resazurin (Alamar Blue) assay; fitting a sigmoid curve on the data points using Microcal ™Origin2021 software | Download PDF |
| Neutrophils | 0.39 ± 0.03 μM | Luminol-dependent chemiluminescence (LCL) assay. | Download PDF |
| Fibroblasts | 20 μM | Cell Counting Kit-8 (CCK-8) | Download PDF |
| B16-F10 | 13.7 µM | Scratch assay | Download PDF |
| HK-2 cells | 50.75 μM | CCK-8 assay, CompuSyn software | Download PDF |
| PMN human leukocytes | 0.18 ± 0.01 µM (against CIP-induced ROS); 0.13 ± 0.01 µM (ag | H2-DCFDA assay | Download PDF |
| MOLM14 cells | 24.49 μM | Four-parameter logistic curve fitting based on eFluor™506 Viability Dye staining and flow cytometry | Download PDF |
| HEK293 | 175.96 ± 1.55 µM | MTT assay | Download PDF |
| HCT 116 | 3.7 µM (48 h), 2.1 µM (72 h) | MTT assay | Download PDF |
| hepatic stellate cells (HSCs) | 27.2 μM | proliferation inhibition | Download PDF |
| OA chondrocytes | 11.58 μM | CCK-8 assay | PMC8291879 |
| A549 cells | 22 μM | Cell viability determined using IN Cell Analyzer 2200 (Hoechst for total nuclei count and ethidium homodimer I for dead cells). IC50 values interpolated from fitted curves using Prism 6.0. | Download PDF |
| BxPC3 cells | 37 μM | Cell viability determined using IN Cell Analyzer 2200 (Hoechst for total nuclei count and ethidium homodimer I for dead cells). IC50 values interpolated from fitted curves using Prism 6.0. | Download PDF |
| S. mutans | 2 mg/mL | MIC and MBC assay | PMC9618393 |
| S. sobrinus | 1 mg/mL | MIC and MBC assay | PMC9618393 |
| L. acidophilus | 2 mg/mL | MIC and MBC assay | PMC9618393 |
| S. sanguinis | 2 mg/mL | MIC and MBC assay | PMC9618393 |
| A. actinomycetemocomitans | 1 mg/mL | MIC and MBC assay | PMC9618393 |
| P. intermedia | 4 mg/mL | MIC and MBC assay | PMC9618393 |
| SKOV-3 | 23.4 µM | MTT assay | PMC5961470 |
| NCI/ADR | 320 µM | MTT assay | PMC5961470 |
| RAW 264.7 cells | 12.85 µg/mL (42.52 µM) | Nitric oxide production inhibitory assay (Griess reagent) | Download PDF |
| AGS | 12.16 µM | CCK-8 assay | Download PDF |
| MGC803 | 8.548 µM | CCK-8 assay | Download PDF |
| RD cells | 291.2 μM | CCK-8 assay | Download PDF |
| Primary human hepatocytes | 74.18 mg/mL | 3D collagen culture, 48 h exposure + 7 d recovery, trypan blue exclusion using a hemocytometer. | Download PDF |
| M. smegmatis mc2155 cells | 312.5 μM (48 hours); 225 μM (72 hours) | MTT assay | Download PDF |
| CT26 cells | 15.67 μg/mL (Qu-M); 24.87 μg/mL (free-Qu) | MTT assay | PMC4368034 |
| CEM cells | 117.4 μM (24h), 25 μM (48h), 15 μM (72h) | CCK-8 assay | PMC12903035 |
| NALM-6 cells | 20 μM | CCK-8 assay | PMC12903035 |
| Vero E6 cells | 83.4 mM | Luciferase assay | PMC7833040 |
| hESCs | 45.35 μM | CCK-8 assay | Download PDF |
| HEC-1-A cells | 104.2 μM (24h), 59.69 μM (48h) | CCK-8 detection of cell activity | Download PDF |
| A2780 | 192.1 µM | CCK-8 analysis, nonlinear regression fitting | PMC12924438 |
| SKOV3 | 83.55 µM | CCK-8 analysis, nonlinear regression fitting | PMC12924438 |
| PC-3 cells | 50.9 µM | MTT assay | PMC6970612 |
| HeLa cells | 4.0 µM | MTT assay | Download PDF |
| HEK cells | 0.12 mM | MTT assay | Download PDF |
Jump to
- Quercetin (Sophoretin) Mechanism of Action: PI3K Inhibition in Cancer Cell Research
- Quercetin (Sophoretin) Clinical Trials
- Quercetin (Sophoretin) Working Concentration | Cell Culture | Treatment
- Quercetin (Sophoretin) Solubility in DMSO
- Quercetin (Sophoretin) Stock Solution
- Quercetin (Sophoretin) Storage
- Quercetin (Sophoretin) Stability
- Quercetin (Sophoretin) Combination Database
- Quercetin (Sophoretin) Molecular Weight
- Quercetin (Sophoretin) CAS Number
- Quercetin (Sophoretin) Chemical Structure (2D and 3D)
- Quercetin (Sophoretin) IC50 for Cell-based and Cell-free Assays
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