Cell-free Assays (IC50/Ki) for Quercetin (Sophoretin)

Key TargetIC50 (Cell-free)DescriptionReference
HIV protease 58.8 µM Protease inhibition assay Download PDF
Mpro of SARS-CoV-1 19180 nM DeepScreeing server using Recurrent Neural Networks (RNN) computing system to develop an inhibitory predictive model using SARS coronavirus Mpro dataset, with PubChem fingerprint for molecular fingerprints. PMC10008098
15-LOX enzyme 61.82±5.5 µg/mL 15-LOX inhibition assay, measuring absorbance at 234 nm. PMC7591100
CaMKIV 61.3 μM BIOMOL® Green reagent-based microtitre plate assay, measuring inorganic phosphate released from ATP. Download PDF
HER2 kinase 570.07 nmol/L HTRF kinEASE TK kit, fluorescent signals correlated with ATP, nonlinear regression with normalized dose-response fit. PMC8088928

Cell-based Assay (IC50) for Quercetin (Sophoretin)

Cell TypeIC50 (Cell-based)DescriptionReference
Vero cells 35.7 µg/mL Inhibition of DENV-2 replication, DENV-2 RNA reduction Download PDF
GES-1, AGS, HGC27, NCI-N87 GES-1: 327.31 µmol/L, AGS: 41.61 µmol/L, HGC27: 26.76 µmol/L CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) Kit PMC11479523
AGS (control), HGC27 (control), NCI-N87 (control), AGS (NUF2 depleted), HGC27 (NUF2 depleted), NCI-N87 (NUF2 depleted) Control AGS: 42.82 µmol/L, Control HGC27: 29.51 µmol/L, Cont CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) Kit PMC11479523
MKN-45 cells 19.27 μmol/L CCK-8 assay, absorbance measured at 450 nm, cell viability expressed as percentage of control values. PMC8088928
MCF-10AT 52.39 µM MTT method, absorbance at 490 nm ([Vendor]) Download PDF
Check the Quercetin (Sophoretin) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

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