CAS No. 675576-98-4

Batch: Purity:99.79
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Nutlin-3a Chemical Structure
Chemical Structure
Check the Nutlin-3a product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 4-[(4S,5R)-4,5-bis(4-chlorophenyl)-2-(4-methoxy-2-propan-2-yloxyphenyl)-4,5-dihydroimidazole-1-carbonyl]piperazin-2-one
CAS Number 675576-98-4
Molecular Formula

C30H30Cl2N4O4
Molecular Weight (MW) 581.49
SMILES CC(C)OC1=C(C=CC(=C1)OC)C2=NC(C(N2C(=O)N3CCNC(=O)C3)C4=CC=C(C=C4)Cl)C5=CC=C(C=C5)Cl
InChIKey BDUHCSBCVGXTJM-WUFINQPMSA-N

Ordering Information

Biological Activity

Nutlin-3a is a small-molecule MDM2 antagonist that inhibits the interaction between p53 and MDM2 with an IC50 of 90 nM in cell-free assays. By disrupting p53/MDM2 binding, Nutlin-3a stabilizes intracellular p53 and activates downstream p53-dependent signaling pathways. Consequently, this p53 activation leads to cell cycle arrest, autophagy, and apoptosis in p53-wild-type cancer cells. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (171.97 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 100 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.